Sardiniella guizhouensis Y.Y. Chen & Jian K. Liu. Phytotaxa 508 (2): 190. (2021).

Li, Wen-Li, Liang, Rui-Ru, Dissanayake, Asha J. & Liu, Jian-Kui, 2023, Botryosphaerialean fungi associated with woody oil plants cultivated in Sichuan Province, China, MycoKeys 97, pp. 71-116 : 71

Sardiniella guizhouensis Y.Y. Chen & Jian K. Liu. Phytotaxa 508 (2): 190. (2021).

Fig. 16

Description.

Saprobic on decaying branches of Pistacia chinensis . Sexual morph: Not observed. Asexual morph: Conidiomata 223-232 × 150-176 μm (x̄ = 227.5 × 163 μm, n = 10), dark brown to black, globose, submerged in the substrate, partially erumpent at maturity, ostiolate. Ostiole 28.5-45 μm diam., circular, central. Peridium 21-30 μm thick, composed of dark brown thick-walled cells of textura angularis, becoming thin-walled and hyaline towards the inner region. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 6-9.5 × 3.5-5 μm (x̄ = 7.5 × 4 μm, n = 20), hyaline, short obpyriform to subcylindrical, holoblastic, indeterminate. Conidia 20.5-24 × 11.5-14 μm (x̄ = 22 × 13 μm, n = 30), L/W ratio = 1.6, ellipsoid to ovoid with both ends rounded, hyaline, aseptate, externally smooth, internally finely verruculose.

Culture characteristics.

Conidia germinate on PDA within 12 h. Colonies growing on PDA, reaching a diameter of 7 cm after five days at 25 °C, effuse, velvety, with entire to slightly undulate edge. Surface initially white and later turning dark olivaceous from the surrounding of the colony and dark gray in reverse.

Material examined.

China, Sichuan Province, Chengdu City, Pidu District , 29°16'50.70"N, 102°37'47.53"E, elevation 442 m, on dead branches of Pistacia chinensis , 19th March 2021, W.L Li, 047 (HUEST 22.0101), living culture UESTCC 22.0100 ; ibid., 070 (HUEST 22.0102), living culture UESTCC 22.0101 ; ibid., 071 (HUEST 22.0100), living culture UESTCC 22.0099 ; ibid., 150 (HUEST 22.0098), living culture UESTCC 22.0097 ; ibid., 151 (HUEST 22.0099), living culture UESTCC 22.0098 ; ibid., A39 (HUEST 22.0103), living culture UESTCC 22.0102 ; ibid., A40 (HUEST 22.0104), living culture UESTCC 22.0103. Additional sequences: LSU: OQ164842 (UESTCC 22.0100), OQ164843 (UESTCC 22.0101), OQ164844 (UESTCC 22.0099), OQ164845 (UESTCC 22.0097), OQ164846 (UESTCC 22.0098), OQ164847 (UESTCC 22.0102) .

Notes.

Seven isolates of our collection are morphologically similar to the original description of Sardiniella guizhouensis ( Chen et al. 2021). The multi-gene phylogenetic analysis showed that the newly obtained isolates clustered together with ex-type of Sa. guizhouensis (CGMCC 3.19222) and this is the first report of Sa. guizhouensis from Pistacia chinensis .

Figures

Figure 16. Sardiniella guizhouensis (HUEST 22.0100, new host record) a-c appearance of conidiomata on natural substrate d vertical section of conidioma e section of peridium f ostiole g-j conidiogenous cells and developing conidia k-n conidia. Scale bars: 40 μm (d); 20 μm (e, f); 10 μm (g-n).