Alternaria burnsii Uppal, Patel & Kamat, Indian J. Agric. Sci.

Nwe, Zin Mar, Htut, Khin Nayyi, Aung, Sein Lai Lai, Gou, Ya-Nan, Huang, Cheng-Xin & Deng, Jian-Xin, 2024, Two novel species and a new host record of Alternaria (Pleosporales, Pleosporaceae) from sunflower (Compositae) in Myanmar, MycoKeys 105, pp. 337-354 : 337-354

Alternaria burnsii Uppal, Patel & Kamat, Indian J. Agric. Sci. 8: 61 (1938)

Fig. 4

Description.

Colonies on PDA are dark, surface buff to honey, cottony to vinaceous buff, with a united margin, measuring 62–64 mm in diameter (Fig. 4 a ). On PCA, conidiophores are single, arising laterally from hyphae, and are either straight or curved, 15–110 (– 115) × (3–5.5) μm. Conidia emerge from the apex and are ovoid or ellipsoid with a tapering beak, 16–42 (– 50) × 5–15 μm, with 2–6 transverse septa, 2–6 in a chain, and beaks are 2–30 (– 40) μm (Fig. 4 c, e, g ). On V 8 A, conidiophores 12–95 (– 103) × (2–4) μm, conidia 9–55 (– 65) × 7–12 μm, and 2–6 transverse septa, 2–9 in a chain, beaks 2–23 (– 35) μm (Fig. 4 b, d, f ).

Additional isolate examined.

In Myanmar, Mandalay Region, Yamethin Township, Segyi Village (30 ° 21 ' 28.188 " N, 112 ° 08 ' 32.136 " E), samples showing disease symptoms on Helianthus annuus were collected in August 2023 by Khin Nayyi Htut. The living culture is designated as YZU 231747 .

Notes.

A. burnsii has been found in many countries on different hosts and substrates. The host range of A. burnsii is reported to include Apiaceae : Cuminum cyminum ( Uppal et al. 1938) , Bunium persicum ( Mondal et al. 2002) , Apium graveolens ( Zhang 2003; Zhuang 2005), Cumin ( Shekhawat et al. 2013); Cucurbitaceae : Cucurbita maxima ( Paul et al. 2015) , Triticum aestivum and Phoenix dactylifera ( Al-Nadabi et al. 2018) , Coconut ( Sunpapao et al. 2022), Phoenix dactylifera ( Al-Nadabi et al. 2020) ; Liliaceae : Allium cepa ( Htun et al. 2022) , and Orchidaceae : Bletilla striata ( Yin et al. 2023) . In the present study, A. burnsii was firstly reported from Helianthus annuus in Myanmar. Phylogenetically, the present strains fall into a sub-branch with A. burnsii YZU 191003 from Allium cepa reported in Myanmar with consistent morphology and nucleotide sequences of ITS, GAPDH, RPB 2, TEF 1, and Alt a 1, gene regions ( Htun et al. 2022) (Fig. 1 ).

Figures

Figure 1. Phylogenetic tree generated from maximum likelihood analyses using aligned ITS, GAPDH, RPB 2, TEF 1, Alt a 1, EndoPG, and OPA 10-2 gene sequences of the present Alternaria strains and their related species. Bootstrap support (BS) values ≥ 60 % and Bayesian posterior probability (PP) scores ≥ 0.60 were shown at the nodes (ML / PP). Alternaria alternantherae CBS 124392 was used as an outgroup. Type strains are marked ‘ T’. Representative strains are marked ‘ R’. The strains from the present study are highlighted in bold.

Figure 4. Morphology of Alternaria burnsii from Helianthus annuus: Colony on PDA for 7 days at 25 ° C (a); Sporulation patterns on V 8 A (b) and on PCA (c); Conidiophores on V 8 A (d) and on PCA (e); Conidia on V 8 A (f) and on PCA (g) at 22 ° C. Scale bars: 50 μm (b, c); 25 μm (d – g).