Mycetophila uninotata Zetterstedt, 1852

Juergenstein, Siiri, Kurina, Olavi & Poldmaa, Kadri, 2015, The Mycetophilaruficollis Meigen (Diptera, Mycetophilidae) group in Europe: elucidating species delimitation with COI and ITS 2 sequence data, ZooKeys 508, pp. 15-51 : 32-35

Taxon classification Animalia Diptera Mycetophilidae

Mycetophila uninotata Zetterstedt, 1852 Figs 14, 31, 32, 49, 50

Material.

NORWAY. 2♂♂, Troms, Svensby, 69°40'01,2"N, 019°49'58,8"E, 18.vii. 2008, sweeping, O. Kurina leg. (IZBE0200113, IZBE0200072, in alcohol with terminalia in glycerine, abdomen used for DNA sequence: SJ303, SJ304). FINLAND. 3♂♂, Sodankylä, Paistipuolet, 75°319'15"N, 34°66'98,8"E, 1. vi– 29.vi.2009, sweeping, J. Salmela leg. (IZBE0200114, IZBE0200115, IZBE0200116, in alcohol with terminalia in glycerine, abdomen used for DNA sequence: SJ436, SJ438, SJ439). ESTONIA. 1♂, Põlva county, Piusa cave, 57°54'N, 27°28'E, 1.ii.1996, sweeping, O. Kurina leg. (IZBE0200241, pinned with terminalia in glycerine); 1♂, Jõgeva county, Pataste, 58°34'52,2"N, 26°46'42,3"E, 10. ix– 20.ix.2008, Malaise trap, J. Elts leg. (IZBE0200151, in alcohol with terminalia in glycerine, abdomen used for DNA sequence: SJ243); 1♂, Harju county, Üksnurme, 59°17'42,5"N, 24°37'41,1"E, 22.ix-12.x.2008, Malaise trap, E. Ilumäe leg. (IZBE0200117, in alcohol with terminalia in glycerine; abdomen used for DNA sequence: SJ227). SLOVAKIA. 1♂, NP Slovensky kras, Silickà Ladnica, 48°33'00,0"N, 020°30'14,4"E, 505m, 4.vi.2009, sweeping, O. Kurina leg. (IZBE0200118, in alcohol, abdomen used for DNA sequence: SJ320).

Male terminalia.

Posterior margin of gonocoxite with shallow convexity ventromedially, and with blunt and oblique projections laterally. Posterior impression very wide and compressed, with emarginated anterior margin. Anterior impression with anteriorly evenly divergent narrow arms. Ventral branch of gonostylus with asymmetrical, narrow and elongated posterior process with dense and long warts; spine 1 sharply pointed; spine 2 about the same size, pointed; spines 3 and 4 smaller, pointed, closer to each other than to spine 2. Dorsal branch of gonostylus steeply tapering; lateral margin with shallow concavity; distal posterior process and proximal posterior process about the same height, both separated by a deep notch. Distal posterior process with 1-2 apical small setae and one bigger subapical seta; proximal posterior process bare and angular. Posterior margin proximally from medial bristle with 3-4 bigger gradually diminishing bristles followed by 2-3 smaller setae; internal surface with a stronger seta next to the medial bristle. Basal angle almost right-angled; basal margin with few setae. Ejaculatory apodeme with campanulate base and without rim. Aedeagus mostly triangular-shaped, apically widened. Aedeagal guides: 1) with two lamellae, 2) with wide and shallow lateral impressions, and 3) apically rounded, not extending beyond aedeagus distally. Aedeagal apodemes laterally angular.

Intraspecific variation.

The wide and compressed posterior impression of the gonocoxite, the distinct warts on the posterior process of the ventral branch of gonostylus and almost equal posterior processes of the dorsal branch of the gonostylus al low the species to be safely distinguished. In Finnish material, spine 2 on the ventral branch of the gonostylus is more massive than described by Laštovka (1972). In some specimens the base of the ejaculatory apodeme and the lateral margin of the dorsal branch of the gonostylus resemble those of Mycetophila ichneumonea . Phylogenetic analysis based on molecular data revealed three different clades (Fig. 4) that cannot be distinguished based on morphology (see also Discussion).

Hosts and distribution.

Mycetophila uninotata has been reared from species of Collybia , Cortinarius and Lactarius ( Yakovlev 1994). Widely distributed in Central and Northern Europe but seems to be absent in the Mediterranean ( Chandler 2013). The distribution gap between Central and Eastern Europe (e.g. absence in Poland, Belarus, Ukraine) can be explained by insufficient collecting.