Bacillus maritimus, Pal & Mathan Kumar & Kaur & Kumar & Kaur & Singh & Krishnamurthi & Mayilraj, 2017

Pal, Deepika, Mathan Kumar, Rajendran, Kaur, Navjot, Kumar, Narender, Kaur, Gurwinder, Singh, Nitin Kumar, Krishnamurthi, Srinivasan & Mayilraj, Shanmugam, 2017, Bacillus maritimus sp. nov., a novel member of the genus Bacillus isolated from marine sediment, International Journal of Systematic and Evolutionary Microbiology 67 (1), pp. 60-66 : 64-65

DESCRIPTION OF BACILLUS MARITIMUS SP. NOV.

Bacillus maritimus (ma.ri′ ti.mus. L. masc. adj. maritimus maritime, marine).

Cells are Gram-stain-positive, rod-shaped, endospore-forming (bulging sporangia) and aerobic. Tolerates up to 7 % (w/v) NaCl (optimum 5 %). No growth occurs in the presence of>8.0 % (w/v) NaCl. The temperature range for growth is 12– 42 Ǫ C (optimum 30 Ǫ C). Growth is observed in the pH range 7.0-11 (optimum pH 8.0) but no growth is observed at pH below 6.0. Negative for hydrolysis of casein, starch and gelatin. Nitrate is reduced to nitrite; H 2 S is not produced. Acid is produced from fructose, raffinose, lactose and melibiose but not from adonitol, dulcitol, dextrose, galactose, inositol, inulin, mannitol, mannose, maltose, rhamnose,, sucrose, salicin, sorbitol, trehalose or xylose. Positive for arginine dihydrolase 1, urease, sucrose, trehalose, raffinose, maltose, L- lactate alkalinization, Oi -galactosidase, L- proline arylamidase, Oi -glucosidase and arginine dihydrolase 2, but negative for β -glucosidase, β - galactopyranosidase, β -galactosidase, salicin, optochine resistance, D- amygdalin, phosphatidylinositol phospholipase C, D- xylose, Ala–Phe–Pro arylamidase, cyclodextrin, L- aspartate arylamidase, Oi -mannosidase, phosphatase, leucine arylamidase, L- pyrrolidonyl-arylamidase, β -glucuronidase, alanine arylamidase, tyrosine arylamidase, D- sorbitol, polymixin B resistance, D- galactose, D- ribose, lactose, N -acetyl-D- glucosamine, bacitracin resistance, novobiocin resistance, growth with 6.5 % (w/v) NaCl, D- mannitol, D- mannose, methyl β -D-glucopyranoside, pullulan and 0/129 resistance (comp.vibrio.). Major fatty acids are iso-C 15: 0, anteiso-C 15: 0, iso-C 14: 0 and iso-C 17: 1 I and/or anteiso-C 17: 1 B. The only menaquinone present is MK-7. The major phospholipids are diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.

The type strain, KS16-9 T (= MTCC 12305 T = DSM 100413 T = KCTC 33834 T), was isolated from a marine sediment sample collected from Kovalam , Kanyakumari coastal region of the Indian Ocean, India. The DNA G+C content of the type strain is 45.4 mol%.