Polypedilum (Polypedilum) tamanigrum Sasa, 1983

Mukherjee, Tuhar, Mukherjee, Bindarika & Hazra, Niladri, 2020, Revision of the Oriental species of Polypedilum Kieffer (Diptera: Chironomidae) with their phylogenetic relationship, Zootaxa 4820 (1), pp. 31-69 : 43

Polypedilum (Polypedilum) tamanigrum Sasa, 1983

GenBank accession no. MN637874

Polypedilum (Polypedilum) tamanigrum Sasa, 1983: 14 .

Material examined. Holotype: Digital archive, National Museum of Nature and Science, Tokyo, Japan; 3 males labelled ‘ Polypedilum (P.) tamanigrum Sasa, 1983 , West Bengal, Darjeeling, Takdah (27.03, 88.36), 05.vi.2019, Coll. D. Mondal’. Retrieval and examination of illustrations of three types including holotype from the digital archive of National Museum of Nature and Science, Tokyo, Japan.

Remarks ( Figure 9 ). Examination of three Indian specimens along with the holotype retrieved from the digital archive of National Museum of Nature and Science, Tokyo, Japan and aligning the COX1 sequence in NCBI, it may be said that the present species fully conforms with those studied in this investigation with the following combination of characters: AR 0.65–0.68, DC 12–14, Sct 8–10, biserial, superior volsella 0.078 –0.081 mm long with middle outer lateral seta and two basal setae, and inferior volsella 0.093 –0.095 mm long with 8 apical setae. The sequence has showed ~1% divergence with MG949983.1 ( Figure 9B ).

Figures

FIGURES 9A–B. Polypedilum (Polypedilum) tamanigrum Sasa, 1983. A, Hypopygium (Photograph) of adult male. Scale: 0.1 mm; B, Neighbor-Joining tree based on the COX1 gene sequences of Polypedilum (Polypedilum) tamanigrum and 28 reference sequences of chironomine midges from GenBank. Red circles mark nodes. Scale: 0.01 substitutions per nucleotide position.