Talaromyces guizhouensis B.D. Sun, A.J. Chen, Houbraken & Samson, 2020

Sun, Bing-Da, Chen, Amanda J., Houbraken, Jos, Frisvad, Jens C., Wu, Wen-Ping, Wei, Hai-Lei, Zhou, Yu-Guang, Jiang, Xian-Zhi & Samson, Robert A., 2020, New section and species in Talaromyces, MycoKeys 68, pp. 75-113 : 75

Talaromyces guizhouensis B.D. Sun, A.J. Chen, Houbraken & Samson sp. nov. Fig. 9

Typus.

China, Guizhou, soil, 2014, isolated by X.Z. Jiang, Holotype CBS H-22835, culture ex-holotype CBS 141837= DTO 340-G8.

Additional material examined.

Malaysia, Langkawi, soil from rainforest, 2007, isolated by J. Houbraken, culture DTO 054-C8. Malaysia, Langkawi, soil from rainforest, 2007, isolated by J. Houbraken, culture DTO 054-A7.

ITS barcode.

MN864277. Alternative identification markers: BenA = MN863346, CaM = MN863323, RPB2 = MN863335.

Diagnosis.

Talaromyces guizhouensis grows poorly on CREA and DG18, does not produce synnemata as well as ascospores.

In.

Talaromyces section Subinflati

Colony diam, 7 d (mm).

CYA 8-9; CYA 30 °C 10; CYA 37 °C No growth; MEA 24-27; MEA 30 °C 18-19; OA 27-29; YES 12-13; CREA 2-3; CYAS No growth; DG18 4-5.

Colony characters.

CYA 25 °C, 7 d: Colonies moderately deep, plane; margins entire; mycelium white; texture floccose; sporulation absent; soluble pigments absent; exudates clear droplets; reverse saffron (10). MEA 25 °C, 7 d: Colonies moderately deep, raised at center, plane; margins entire; mycelium white; texture floccose; sporulation moderately dense; conidia en masse pistachio green (92); soluble pigments absent; exudates absent; reverse saffron (10). YES 25 °C, 7 d: Colonies moderately deep, raised at center, plane; margins entire; mycelium white; texture floccose; sporulation absent; soluble pigments absent; exudates clear droplets; reverse cream white. DG18 25 °C, 7 d: Colonies moderately deep, plane; margins entire; mycelium white; texture floccose; sporulation absent; soluble pigments absent; exudates absent; reverse cream white. OA 25 °C, 7 d: Colonies moderately deep, raised at center, plane; margins entire; mycelium white; texture floccose; sporulation moderately dense; conidia en masse pistachio green (92); soluble pigments absent; exudates clear droplets; reverse greyish lavender (98) at center, fading into saffron (10). CREA 25 °C, 7 d: Poor growth, acid production absent.

Micromorphology.

Conidiophores biverticillate, stipes smooth to finely rough, 150-300 × 3-4.5 μm, metulae 3-5, divergent, 11-13 × 3-5 μm; phialides 3-5, acerose to flask shaped, 9-10 × 3-3.5 μm; conidia finely rough, subglobose to fusiform, 2.5-4.5 × 2.5-3 μm. Ascomata not observed.

Notes.

section Subinflati previously contained two species namely T. subinflatus and T. palmae . These species do not resemble each other, although both grow poorly on CREA and DG18 ( Yilmaz et al. 2014). Talaromyces tzapotlensis was included more recently ( Peterson and Jurjević 2017) and we here expand this section with T. guizhouensis and T. resedanus . Like the other species in this section, T. guizhouensis also grows poorly on CREA and DG18. This species is phylogenetically related to T. subinflatus , but the latter grows very restrictedly on common media except MEA ( Yilmaz et al. 2014). Talaromyces palmae produces indeterminate synnemata and short stipes (up to 85 μm) ( Yilmaz et al. 2014) and these are not observed in T. guizhouensis . Furthermore, T. tzapotlensis grows faster on most media (e.g., 29-30 vs 8-9 mm on CYA; 10-11 vs 4-5 mm on DG18; 20-22 vs 2-3 mm on CREA, all diam. after 7 days ( Peterson and Jurjević 2017) and T. resedanus does not grow on CREA and produces smaller conidia measuring 2-3 × 1.5-2 μm.

Etymology.

Latin, guizhouensis , refers to its origin, isolated from Guizhou, China.

Figures

Figure 9. Talaromyces guizhouensis CBS 141837 T a colonies from left to right (top row) CYA, MEA, YES and OA; (bottom row) CYA reverse, MEA reverse, DG 18 and CREA b-g conidiophores and conidia. Scale bars: 10 μm (b-g).