Cylindrocladiella malesiana N.Q. Pham & M.J. Wingf.

Pham, Nam Q., Barnes, Irene, Chen, ShuaiFei, Pham, Thu Q., Lombard, Lorenzo, Crous, Pedro W. & Wingfield, Michael J., 2018, New species of Cylindrocladiella from plantation soils in South-East Asia, MycoKeys 32, pp. 1-24 : 1-4

Cylindrocladiella malesiana N.Q. Pham & M.J. Wingf. sp. nov. Figure 3

Etymology.

Name refers to Malaysia, the country where this species was first collected.

Type material.

MALAYSIA. Sabah State: Tawau, Brumas, from soil in Acacia mangium plantation, Mar. 2013, M.J. Wingfield, herbarium specimen of dried culture, PREM 62161 (holotype), CMW 48278 = CBS 143548 (ex-type culture).

Description.

Sexual morph not observed. Conidiophores dimorphic, penicillate and subverticillate, mononematous and hyaline. Penicillate conidiophores comprising a stipe, a penicillate arrangement of fertile branches, a stipe extension and a terminal vesicle; stipe septate, hyaline, smooth, 76.5-126 × 3.5-5 µm; stipe extension aseptate, straight, 114.5-144.5 µm long, thick-walled with one basal septum, terminating in thin-walled, fusoid to lanceolate vesicles, 4.5-6 µm wide. Penicillate conidiogenous apparatus with primary branches aseptate, 16.5-24 × 3-4.5 µm, secondary branches aseptate, 10.5-15 × 2-3.5 µm, each terminal branch producing 2-4 phialides; phialides cymbiform to cylindrical, hyaline, aseptate, 9-15.5 × 2-3.5 µm, apex with minute periclinal thickening and collarette. Subverticillate conidiophores abundant, comprising of a septate stipe and primary branches terminating in 2-4 phialides; primary branches straight, hyaline, 0-1-septate, 13.5-35 × 2.5-4 µm; phialides cymbiform to cylindrical, hyaline, aseptate, 14.5-27 × 2-3.5 µm, apex with minute periclinal thickening and collarette. Conidia cylindrical, rounded at both ends, straight, 1-septate, (10 –)11–13(– 13.5) × (1.5 –)2– 2.5 µm (av. = 12 × 2 µm), frequently slightly flattened at the base, held in asymmetrical clusters by colourless slime.

Culture characteristics.

Colonies buff to hazel on the surface and dark brick to brown vinaceous in reverse on MEA after 7 d; smooth to undulate margins; moderate aerial mycelium; chlamydospores moderate, arranged in chains. Optimal growth temperature at 25 °C, no growth at 5 °C and 35 °C; after 7 d, colonies at 10 °C, 15 °C, 20 °C, 25 °C and 30 °C reached 3.8 mm, 24.3 mm, 45.2 mm, 74.4 mm and 48.8 mm, respectively.

Distribution.

Sabah, Malaysia

Additional material examined.

MALAYSIA. Sabah state: Tawau, Brumas, from soil in Acacia mangium plantation, Mar. 2013, M.J. Wingfield, PREM 62162, culture CMW 48276 = CBS 143549; ibid., PREM 62163, culture CMW 48277 = CBS 143550.

Notes.

Cylindrocladiella malesiana is phylogenetically closely related to Cy. microcylindrica , Cy. natalensis and Cy. solicola . Conidia of Cy. malesiana are shorter than those of Cy. microcylindrica , Cy. natalensis and Cy. solicola (Table 2).