Longidorus bordonensis, Gutiérrez-Gutiérrez & Santos & Inácio & Eisenback & Mota, 2020
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publication ID |
https://dx.doi.org/10.3897/zse.96.49022 |
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publication LSID |
lsid:zoobank.org:pub:16388413-BF9F-4339-AF96-1179BB8CED1D |
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persistent identifier |
https://treatment.plazi.org/id/D753E7C6-512E-4A69-B9FE-326B9783D74C |
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taxon LSID |
lsid:zoobank.org:act:D753E7C6-512E-4A69-B9FE-326B9783D74C |
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treatment provided by |
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scientific name |
Longidorus bordonensis |
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sp. nov. |
Longidorus bordonensis sp. nov. Figs 1 View Figure 1 (1-7), 2(1-10), Table 2 View Table 2 , Suppl. material 4: Table S2)
Holotype.
Slide PLB001.
Paratypes.
6 females and 6 males (slides PLB002-PLB 013) mounted on glass slides.
Type repositories.
The holotype (PLB001) and 8 paratypes (4 females and 4 males) (slides PLB002-PLB005 and PLB008-PLB011) are deposited in the Nematode Collection of the Nematology Lab, Institute for Mediterranean Agricultural and Environment Sciences, ICAAM, University of Évora, Évora; 2 paratypes (1 female and 1 male) (slides PLB006 and PLB012) in the Royal Belgian Institute of Natural Sciences, Brussels, Belgium; 2 paratypes (1 female and 1 male) (slides PLB007 and PLB013) in the Istituto per la Protezione delle Piante (IPP) of Consiglio Nazionale delle Ricerche (C.N.R.), Sezione di Bari, Bari, Italy.
Type locality.
Holotype and paratype specimens were extracted from a soil sample collected from the rhizosphere of an unidentified grass species at Bordonhos, São Pedro do Sul, Viseu district, Beira Alta province, northern Portugal (40°45'53"N, 8°5'12"W) (Table 1 View Table 1 )
Etymology.
The specific epithet of this species refers to the region of the type locality (Bordonhos) where the new species was found.
Description of female.
Short and slender body, slightly tapering at both ends, more pronounced in the tail. Curved in open J- or C-shaped relaxed by heat. Cuticle thin, appearing smooth under low magnifications, 1.8 ± 0.3 (1.3-2.2) μm thick at mid body, but thicker (9.1 ± 0.7 (8.1-9.8) μm) in hyaline region located at the end of tail region (Figs 1 View Figure 1 (3), 2(6, 7); Table 2 View Table 2 ). Lateral chord ca 11.1 μm wide at mid-body or ca 34% of corresponding body diam. Lip region anteriorly flattened, expanded and rounded laterally, 10.1 ± 0.4 (9.6-10.7) μm wide and 4.1 ± 0.5 (3.6-5.0) μm high, set-off from body contour by a constriction (Figs 1 View Figure 1 (1, 2), 2(1, 2), Table 2 View Table 2 ). Amphidial fovea large asymmetrically bilobed pouch occupying from 2/3 to 3/4 of the distance from oral aperture to guiding ring (Figs 1 View Figure 1 (1, 2), 2(3), Table 2 View Table 2 ). Stylet guiding-ring single and posteriorly situated, 2.7-2.3 times lip region diameter from anterior end. Moderate and straight odontostyle, 1.5 ± 0.1 (1.3-1.7) times as long as odontophore; weakly developed, with rather weak basal swellings (Figs 1 View Figure 1 (1), 2(4) Table 2 View Table 2 ). Nerve ring surrounding the tubular portion of the pharynx behind the odontophore base at 149.9 ± 7.7 (138.1-157.7) μm from anterior end. Anterior slender part of pharynx usually coiled in its posterior region. Basal bulb short and cylindrical, 79.9 ± 6.2 (68.0-87.2) μm long and 13.5 ± 1.0 (12.1-14.7) μm in diameter. Glandularium 71.9 ± 3.0 (67.0-74.3) μm long. Dorsal pharyngeal gland nucleus (DN) and ventro-sublateral nuclei (SVN) located at 33.1 ± 3.2 (29.4-35.3)% and 53.1 ± 0.7 (52.6-53.9)% of distance from anterior end of pharyngeal bulb, respectively. Cardia conoid to rounded, 8.6 ± 2.7 (6.3-13.1) μm long (Figs 1 View Figure 1 (7), 2(5), Table 2 View Table 2 ). Reproductive system with both genital branches equally developed, 8.4 ± 0.5 (7.5-8.7) or 8.0 ± 1.2 (6.7-9.5)% of body length (Table 2 View Table 2 ). Ovaries reflexed, variable in length, anterior ovary 71.4 ± 14.6 (52.0-85.8 μm long) and posterior ovary 82.3 ± 13.6 (68.0-99.0 μm long) (Table 2 View Table 2 ). Oviducts slightly longer than ovaries. Uteri cylindrical, quite variable in length, anterior uteri 283.9 ± 94.0 (209.0-418.0 μm long) and posterior uteri 251.3 ± 43.7 (194.0-295.0 μm long); sphincter usually well developed, delimiting uterus and oviduct. Sperm commonly found in the uteri of female reproductive tract. Vulva transverse, located slightly anterior to the middle of the body, vagina perpendicular to body axis, 21.8 ± 1.9 (19.0-24.6) μm long (Figs 1 View Figure 1 (5), 2(9), Table 2 View Table 2 ). Prerectum visible, variable in length, 547.5 ± 503.7 (162.0-1201.0) μm long, and short rectum 20.0 ± 2.9 (15.0- 23.0) μm long or 1.2 (0.9-1.6) times anal body width. Tail long, bluntly conoid, slightly ventrally curved with rounded terminus, bearing three pairs of caudal pores (Figs 1 View Figure 1 (3), 2(6, 7); Table 2 View Table 2 ).
Description of male.
Males are as common as females. Appearance of body similar to female, except for reproductive organs. Male diorchic with testes paired and opposed. Tail conoid, more convex-curved ventrally than that of the female, with rounded terminus at the end of tail (Figs 1 View Figure 1 (6), 2(8), Table 2 View Table 2 ). Spicules short, moderately developed, and quite curved ventrally; lateral guiding pieces more or less straight, sometimes with slightly curved proximal ends (Figs 1 View Figure 1 (4, 6), 2(8, 10), Table 2 View Table 2 ). Large number of visible supplements, one pair of adanal and 9-11 mid-ventral supplements (Figs 1 View Figure 1 (3), 2(8)).
Differential diagnosis.
Longidorus bordonensis sp. nov. is characterized by a short body within the genus Longidorus (average = 4443 µm and 4560 µm in females and males, respectively), short odontostyle within the genus Longidorus (average = 70.0 µm and 69.5 µm in females and males, respectively), lip region anteriorly flattened, expanded (average = 10.0 µm in both females and males) and rounded laterally, set-off from body contour by a constriction, asymmetrically bilobed amphidial pouches with lobes occupying from 2/3 to 3/4 part of the distance from oral aperture to guiding ring, tail long (average = 51.0 µm and 55.0 µm in females and males, respectively), bluntly conoid, slightly ventrally curved with rounded terminus, short to medium spicules (average = 37.0 µm) with one pair of adanal and 9-11 mid-ventral supplements (Figs 1 View Figure 1 (1-7), 2(1-10), Table 2 View Table 2 , Suppl. material 4: Table S2). According to the polytomous key of Chen et al. (1997) and two subsequent supplements ( Loof and Chen 1999; Peneva et al. 2013), L. bordonensis sp. nov. has the following codes (codes in parentheses are exceptions): A2, B1, C2, D4, E3, F2(3), G3, H(5)6, I2, J?, K?. On the basis of the diagnostic characters (body length, odontostyle length, lip region width, shape of anterior region, shape of amphidial pouch, oral aperture-guiding ring distance, tail length, spicule length, tail shape, a and c’ ratios, and frequency of males) used in the polytomous key by Chen et al. (1997), and supplements by Loof and Chen (1999) and Peneva et al. (2013), L. bordonensis sp. nov. is grouped with L. indalus Archidona-Yuste, Navas-Cortés, Cantalapiedra-Navarrete, Palomares-Rius & Castillo, 2016, L. carpetanensis Arias, Andrés & Navas, 1986, L. unedoi Arias, Andrés & Navas, 1986, L . juvenilis , L . pini Jacobs & Heyns, 1987, L. pisi Edward, Misra & Singh, 1964, and L. distintus Lamberti, Choleva & Agostinelli, 1983. Morphological and morphometric characters of the new species are compared with its closely related species (Suppl. material 4: Table S2). Longidorus bordonensis sp. nov. differs from paratypes of L. pini by small differences in the distance from the guiding ring to the anterior end (23.0-26.7 μm vs 26-27 μm), amphidial pouch shapes (asymmetrically bilobed with lobes occupying from 2/3 to 3/4 of the oa-gr distance vs symmetrically bilobed with lobes occupying from 1/3 to 2/3 of the oa-gr distance), tail shape (bluntly conoid, slightly ventrally curved with round terminus vs tail long, conical dorsally convex and ventrally concave, with the round terminus slightly subdigitate) and the frequency of males (common vs absent). Also, the new species differs from some previously cited species in measurements and ratios, including L, c’ and a ratios, odontostyle length, lip region diameter and shape, the distance from guiding ring to anterior, and tail length and shape (Suppl. material 4: Table S2).
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