Buwchfawromyces eastonii Callaghan, Tony & G.W. Griff.

Callaghan, Tony Martin, Podmirseg, Sabine Marie, Hohlweck, Daniel, Edwards, Joan Elizabeth, Puniya, Anil Kumar, Dagar, Sumit Singh & Griffith, Gareth Wyn, 2015, Buwchfawromyceseastonii gen. nov., sp. nov.: a new anaerobic fungus (Neocallimastigomycota) isolated from buffalo faeces, MycoKeys 9, pp. 11-28 : 19-20

Taxon classification Fungi Neocallimastigales Neocallimastigaceae

Buwchfawromyces eastonii Callaghan, Tony & G.W. Griff. gen. nov., sp. nov.

Note.

An obligately anaerobic fungus with determinate monocentric thallus and spherical to ovoid sporangia. Thalli often with a distinctly swollen sporangiophore and twisted rhizoids. Extensive rhizoidal system but sporangiphore and rhizoids lacking nuclei. Sporangia ovoid to spherical (30-80 µm × 20-60 µm), non-papillate. Zoospores formed abundantly, spherical (9-11 µm diameter) with single flagellum (30-40 µm long). The reference sequences for this species are EU414755 and EU414756 (ITS1, 5.8S, ITS2), and KP205570 (LSU, D1/D2 regions). Since intragenomic variation in ITS1 sequence is present, the ITS1 sequence Buwchfawromyces eastonii is defined as the least inclusive clade containing both EU414755 and EU414756. The type culture (isolate GE09) is stored cryogenically in liquid nitrogen at Aberystwyth University. Type material from 3 d old cultures and preserved in 5% glutaraldehyde is lodged in the biorepositories at: Aberystwyth University (code ABS) with isotype material at Royal Botanic Gardens, Kew, London (K); and Friedrich-Schiller-Universität Jena, Germany (JE).

Etymology.

From the Welsh words for large cow ('buwch fawr’), since the original isolate GE09 was isolated from a buffalo for which there is no Welsh word. The specific epithet in honour of our former colleague Gary Easton who isolated this fungus.

Nomenclature.

Buwchfawromyces Callaghan, Tony & G.W. Griff., gen. nov. Strictly anaerobic fungus forming a monocentric thallus with a single sporangium, usually borne on a swollen sporangiophore connected to a branching and twisted rhizoidal system. Zoospores are spherical and uniflagellate. "Buwch fawr" means large cow in Welsh.