Diaporthe biguttulata F. Huang, K.D. Hyde & H.Y. Li, 2015

Yang, Qin, Fan, Xin-Lei, Guarnaccia, Vladimiro & Tian, Cheng-Ming, 2018, High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described, MycoKeys 39, pp. 97-149 : 97

Diaporthe biguttulata F. Huang, K.D. Hyde & H.Y. Li, 2015 Figure 6

Description.

Conidiomata pycnidial, immersed in bark, scattered, erumpent through the bark surface, discoid, with a single locule. Ectostromatic disc dark brown, one ostiole per disc, 160-320 μm diam. Locule undivided, 235-350 μm diam. Conidiophores 8.5-11 × 1.5 μm, cylindrical, hyaline, branched, straight or slightly curved, tapering towards the apex. Alpha conidia hyaline, aseptate, ellipsoidal to oval, 2-guttulate, usually rounded at both ends, occasionally with one end acute, 7-8.5 × 1.5-2 μm (av. = 6.5 × 2.6 μm, n = 30). Beta conidia not observed.

Culture characters.

Cultures incubated on PDA at 25 °C in darkness. Colony originally flat with white aerial mycelium, becoming pale grey, with dense aerial mycelium in the centre and sparse aerial mycelium at the marginal area, conidiomata absent.

Specimens examined.

CHINA. Zhejiang Province: Tianmu Mountain, on symptomatic branches of Juglans regia , 20 Apr. 2017, Q. Yang, living culture CFCC 52584 and CFCC 52585 (BJFC-S1504).

Notes.

Diaporthe biguttulata was originally described from a healthy branch of Citrus limon in Yunnan Province, China ( Huang et al. 2015). In the present study, two isolates (CFCC 52584 and CFCC 52585) from symptomatic branches of Juglans regia were congruent with D. biguttulata based on morphology and DNA sequences data (Fig. 1). We therefore describe D. biguttulata as a known species for this clade.