Diatrypella vulgaris Trouillas, W.M. Pitt & Gubler, Fungal Diversity 49: 212 (2011)

Long, Sihan, Liu, Lili, Pi, Yinhui, Wu, Youpeng, Lin, Yan, Zhang, Xu, Long, Qingde, Kang, Yingqian, Kang, Jichuan, Wijayawardene, Nalin N., Wang, Feng, Shen, Xiangchun & Li, Qirui, 2021, New contributions to Diatrypaceae from karst areas in China, MycoKeys 83, pp. 1-37 : 1

Diatrypella vulgaris Trouillas, W.M. Pitt & Gubler, Fungal Diversity 49: 212 (2011)

Fig. 5

Description.

Saprobic on decaying branches of an unidentified plant. Sexual morph: Stromata scattered on the host, 0.8-1.5 mm long and 0.8-2 mm broad (av. = 1.2 × 1.3 mm, n = 30) pustulate, visible as black, rounded to irregular in shape on host surface, semi-immersed, erumpent through host bark, with 2-8 ascomata immersed in one stroma. Endostroma consists of outer dark brown, small, dense, thin parenchymal cells and an inner layer of white, large, loose parenchymal cells. Ostiole opening separately, papillate or apapillate, central 710.7-787.2 μm high, 270.2-422 μm diam. (av. = 742 × 363 μm, n = 10). Perithecium immersed in stroma, round to oblong, with cylindrical neck, brevicollous or longicollous. Peridium composed of outer layer of dark brown to black, thin-walled cells, arranged in textura angularis, inner layer of hyaline thin-walled cells of textura angularis. Asci 111.4-152.9 × 10.6-17.5 μm (av. = 124.5 × 15.5 μm, n = 30), polysporous, clavate, long-stalked, apically rounded. Ascospores 8-11 × 1-2 μm (av. = 8.9 × 1.7 μm, n = 30), overlapping, crowded, allantoid, slightly or moderately curved, smooth, subhyaline, yellowish in mass, aseptate, usually with two oil droplets. Asexual morph: undetermined.

Culture characteristics.

Ascospores germinating on PDA within 24 hours. Colonies on PDA, white when young, became pale brown, dense, but thinning towards edge, medium dense, white from above, reverse side white at margin, flesh to pale brown at centre, no pigmentation produced on PDA medium, no conidia observed on PDA or on OA media.

Specimens examined.

China, Guizhou Province, Guiyang City, Gaopo Township (26°29'72.02"N, 106°29'55.57"E), on branches of unidentified plant, 30 October 2020. Altitude: 1589 m, S.H. Long, GP02 (GMB0051, KUN-HKAS 112697, living culture GMBC0051) .

Additional sequences.

GMB0051 (LSU: MW797051, RPB2: MW814897).

Note.

The comparison of ITS sequences in NCBI showed that this isolate is 100% similar to the strain of Diatrypella vulgaris (HVGRF03), isolated from holotype specimens. Morphologically, GMB0051 shows the same features as Diatrypella vulgaris . The stromata of these specimens are similar, but ascospores of GMB0051 are thinner than those of the HVGRF03 (8-10 × 2-2.5 μm) and, when compared with the ascospores of strain MFLUCC 17-0128 (4.5-7.5 × 1-2 μm), they are shorter than GMB0051 ( Trouillas et al. 2011; Hyde et al. 2017). Here, we use the ITS sequence similarity between the new collection and the type strain of Diatrypella vulgaris as the identification tool. Diatrypella vulgaris has been reported in Austria and Thailand ( Trouillas et al. 2011, Hyde et al. 2017). This is the first report of Diatrypella vulgaris from China.

Figures

Figure 5. Diatrypella vulgaris (GMB 0051, new record for China) A stromata on host substrate; B, C close-up of stroma D transverse sections through ascostroma E vertical section through ascostroma F culture on PDA G section through the ascostroma H, I ostiolar canal J, K asci L-O ascospores. Scale bars: 20 μm (G); 10 μm (H-I).