Fusarium fujikuroi Nirenberg, Mitteilungen der Biologischen Bundesanstalt fuer Land- und Forstwirtschaft 169: 32 (1976)

He, Jiao, Li, De-Wei, Cui, Wen-Li, Zhu, Li-Hua & Huang, Lin, 2024, Morphological and phylogenetic analyses reveal three new species of Fusarium (Hypocreales, Nectriaceae) associated with leaf blight on Cunninghamia lanceolata in China, MycoKeys 101, pp. 45-80 : 45

Fusarium fujikuroi Nirenberg, Mitteilungen der Biologischen Bundesanstalt fuer Land- und Forstwirtschaft 169: 32 (1976)

Description.

Sexual state not observed. Asexual state: Sporulation abundant from sporodochia, rarely from conidiophores formed directly on the substrate mycelium. Conidiophores in the aerial mycelium branched, bearing terminal or intercalary phialides. Phialides subulate to subcylindrical, smooth, thin-walled, (11.5-)14.7-22.9(-30.0) μm × (1.8-)2.0-3.6(-4.0) μm, (mean ± SD = 18.8 ± 4.1 μm × 2.8 ± 0.8 μm, n = 37), without periclinal thickening; microconidia hyaline, short clavate to cylindrical, slender to relatively straight, smooth, thin-walled, 0-septate, (5.4-)6.7-11.3(-15.5) × (2.0-)2.5-3.5(-4.4) μm, (mean ± SD = 9.0 ± 2.3 × 3.0 ± 0.5 μm, n = 81), forming small false heads on the tips of phialides. Chlamydospores formed occasionally, mostly in pairs or chains, terminal or intercalary, globose to subglobose, smooth-walled, (6.0-)6.2-8.0(-8.3) × (4.4-)4.4-5.2(-5.6) μm, (mean ± SD = 7.1 ± 0.9 × 4.8 ± 0.4 μm, n = 6). Sporodochia and macroconidia not observed.

Culture characteristics.

Colonies on PDA growing in the dark with an average growth rate of 13.9 mm/d at 25 °C. Colony surface white to purple, flat or slightly raised at the center; colony round, regular, margins filiform, aerial mycelium abundant. Reverse purple with white periphery. Odor absent. Colonies on SNA incubated at 25 °C in the dark were regular, round, growing at 8.1 mm/d. Colony surface pure white, aerial mycelium absent or scant. Reverse pure white, without diffusible pigments. Colonies on OMA incubated at 25 °C in the dark were regular, round, aerial mycelium abundant, loose to densely floccose, growing at 12.5 mm/d. Colony white to dark purple and with white to dark violet pigmentation. Colonies on CMA incubated at 25 °C in the dark were regular, round, colony surface and reverse white, aerial mycelium absent or scant, growing at 11.3 mm/d.

Materials examined.

China, Hunan province, Yiyang City, Heshan District, Henglongqiao Town , 28°27′24″N, 112°29′7″E, isolated from leaf spots of Cunninghamia lanceolata , May 2017, Wen-Li Cui, isolates: HN43-17-1, HN43-17-1-1, HN43-17-1-2, HN43-17-1-3 .

Notes.

The isolate HN43-17-1 in this study was in the same clade with F. fujikuroi CBS 221.76 (ex-type). Morphologically, 0-septate microconidia, (5.4-15.5 × 2-4.4 μm) of the isolate HN43-17-1 were more variable than the 0-septate microconidia (12.2-12.9 × 3.4-3.7 μm) of the ex-type (CBS 221.76) of F. fujikuroi ( Ibrahim et al. 2016).