Pleurothecium aquaticum Z.L. Luo, H.Y. Su & K.D. Hyde, in Luo, Hyde, Bhat, Jeewon, Maharachchikumbura, Bao, Li, Su, Yang & Su, Mycol. Prog. 17(5): 526 (2018)

Bao, Dan-Feng, Bhat, Darbhe J., Boonmee, Saranyaphat, Hyde, Kevin D., Luo, Zong-Long & Nalumpang, Sarunya, 2022, Lignicolous freshwater ascomycetes from Thailand: Introducing Dematipyriforma muriformis sp. nov., one new combination and two new records in Pleurotheciaceae, MycoKeys 93, pp. 57-79 : 57

Pleurothecium aquaticum Z.L. Luo, H.Y. Su & K.D. Hyde, in Luo, Hyde, Bhat, Jeewon, Maharachchikumbura, Bao, Li, Su, Yang & Su, Mycol. Prog. 17(5): 526 (2018)

Fig. 4

Description.

Saprobic on submerged decaying wood. Sexual morph: Undetermined. Asexual morph: colonies on substratum, effuse, shining, dark brown to black. Mycelium partly immersed, composed of septate, branched, smooth, dark brown hyphae. Conidiophores 84-110 × 3-4 μm (x - = 97 × 3.4 μm, n = 10), macronematous, mononematous, erect, simple, unbranched, straight or slightly flexuous, 5-8-septate, dark brown, pale towards apex, smooth. Conidiogenous cells integrated, polyblastic, terminal, hyaline, denticulate, smooth. Conidia 18-22 × 4-5 μm (x - = 20 × 4.5 μm, SD = 4 n = 30), acrogenous, solitary, clavate, mostly curved, rounded at apex, tapering at base, hyaline, 3-septate, with guttulate cells, smooth.

Cultural characteristics.

Conidia germinating on PDA within 24 h. Germ tubes produced from the basal and apical cells. Colonies on PDA reaching 2.3 cm diameter in 30 days at 20-25 °C, with dense mycelia, dry, rigid, rugose, dark brown, reverse dark brown.

Material examined.

Thailand, Prachuap Khan , on submerged decaying wood, 15 August 2017, V. Kumar, site1-24-2 (MFLU 21-0148 = KUN-HKAS 122857), living culture, KUNCC 21-0477 .

Notes.

In the phylogenetic analysis, our new collection KUNCC 21-0477 clustered with the ex-type strain of Pleurothecium aquaticum (MFLUCC 17-1331) with high (100% ML/1.00 PP). In addition, the ITS and LSU BLASTn search on NCBI GenBank showed that our new strain is 99.88% and 97.45% similarities to the ex-type of P. aquaticum (MFLUCC 17-1331). The new collection is morphologically similar to P. aquaticum in having macronematous, mononematous, septate, brown, pale brown towards the apex conidiophores, integrated, terminal, polyblastic, denticulate conidiogenous cells and hyaline, cylindrical or clavate, rounded at the apex, obtuse and tapering towards base, 3-septate conidia. We therefore identified our new collection as P. aquaticum . Pleurothecium aquaticum was introduced by Luo et al. (2018a) collected from freshwater habitats in China. Our new collection, on the other hand, was collected from Thailand and is a new record for Thailand.

Figures

Figure 4. Pleurothecium aquaticum (MFLU 21 - 0148, new record) a, b colonies on wood c, d conidiophores e, f conidiogenous cells g-i conidia m germinating conidium n, o culture on PDA from surface and reverse. Scale bars: 30 μm (c, d); 10 μm (e-m).