Lophophysema eversa, Gong, Lin, Li, Xinzheng & Qiu, Jian-Wen, 2014

Gong, Lin, Li, Xinzheng & Qiu, Jian-Wen, 2014, A new species of Lophophysema (Porifera, Hexactinellida, Hyalonematidae) from the South China Sea, Zootaxa 3884 (6), pp. 553-560 : 555-559

Lophophysema eversa sp. nov.

( Figures 1–3 )

Lophophysema eversa Zhang, Sun, Li & Qiu, 2014 : Mitochondrial DNA (in press) DOI: 10.3109/19401736.2014.945552 [nomen nudum]

Material examined. Holotype: MBM 179978, northeast of the Zhongsha Islands, South China Sea, 117°45.5'E, 17°36.7’N, 7 July 2013, 3683 m depth, muddy substrate, collected by Xinzheng Li and Jian-Wen Qiu using the “Jiaolong” submersible.

Description. The holotype is half-gourd shaped, composed of two opposite cones, with a smaller upper part and a larger lower part ( Figure 1 ). It is different from the typical shape of genus Lophophysema which always have a larger upper cone. The body length (without stalk) is 182 mm, with a maximum diameter of 76 mm. An everted atrial surface extends from the whole apical cone to the flank of the lower cone diminishedly. The everted atrial surface composed of the upper cone and divide the flank of the body into two parts which is comparatively very closed. The dermal surface between the basalia and the everted atrial surface located at the lower cone. It has large holes and organized into sieve ( Figure 1 B) with mesh size from 1.5 mm to 4.5 mm. The color of the atrial surface and dermal surface is darken yellow and beige, respectively. Furthermore, compared to the dermal surface, the color of the atrial surface looks darker. Large inhalant cavities can be found under the dermal surface. The basal exist on the side of the body. For other Lophophysema spcies, the basal may exist on the middle of body due to it has no special describe on it in Tabachnick & Lévi (1999) and the figure 4 of the Lophophysema body form in Tabachnick & Menshenina. Basalia with smooth surface tightly twisted in a tuft, and stretch from the bottom to protrude over the apex. These were broken as a result of their collection ( Figure 1 C). The smallest diameter of the tuft is 7 mm; individual basalia vary from 0.1 mm to 1 mm in width. Sponge tissues are fleshy, light and compressible, with an orange rusty patch on the lower part wrapping the basalia ( Figure 1 B). The basal stalk is long,> 650 mm, and the part protruding over the apex is 124 mm long. Marginalia were difficult to observe due to the fragmentation of the specimen after its preservation.

Spicules. The choanosomal skeleton has smooth and slightly curved diactins ( Figure 2 ). Diactins are medially widened ( Figure 2 J–L), terminally conical or slightly swollen ( Figure 2 F–I). Acanthophores are present as diactins ( Figure 2 N), stauractins ( Figure 2 O –P), pentactins ( Figure 2 Q), hexactins ( Figure 2 R), with ends covered with small spines. Choanosomal hexactins ( Figure 3 J) are covered with short spines and have relatively longer and thicker rays than microhexactins ( Figure 3 K).

Dermalia, atrialia, and canalaria are pinular pentactins. The tangential rays of pinular pentactins are spiny. Atrialia ( Figure 3 E) are similar to canalaria ( Figure 3 D) but larger. The atrialia pinular rays are 204.9–402.2 µm long, while canalaria pinular rays are 28.8–129.7 µm long (Table 1). The dermalia pinular rays ( Figure 3 C) are 104.3–174.2 µm long and usually spindle-like, with the teeth on the ray being longer and denser than the other two types. The hypodermal pentactins ( Figure 3 B) have smooth rays of varying lengths: 187.6–674.9 µm long (proximal rays), and 75.6–220.7 µm long (tangential rays), pentactins with two reduced rays ( Figure 3 A) may occasionally be present. The hypoatrial pentactins ( Figure 3 F) have long tangential rays (137.3–406.1 µm long) and short proximal rays which are difficult to measure under light microscopy.

Microscleres consist of amphidiscs and microhexactins. Amphidiscs consist of two forms. Macramphidiscs are absent. Mesamphidiscs ( Figure 3 G) have shafts covered by numerous spines, total length 55.2–89.2 µm, umbel length 20.1–34.5 µm, and umbel diameter 16.9–26.9 µm. Micramphidiscs ( Fig. 3 H) have small spiny shafts similar to those of mesamphidiscs, with total length ranging from 12.4–21.2 µm. Micramphidiscs significantly outnumber the mesamphidiscs. Microhexactins ( Figure 3 I, K) have numerous small spines on their rays. These rays ( Figure 3 I) are 14.7–40.6 µm long, mostly straight, although some have slightly curved ends.

Etymology. The specific name eversa is derived from the Latin word "eversus", meaning everted, referring to the notably everted atrial surface.

Remarks. The genus Lophophysema Schulze, 1900 contains three described species: L. gilchristi Tabachnick & Lévi, 1999 , L. australicum Tabachnick & Lévi, 1999 and L. inflatum Schulze, 1900 . In a review of Lophophysema, Tabachnick & Lévi (1999) pointed out the distinct characters of the three species: L. inflatum contains microhexactins; L. australicum has rough monactin and the macramphidisc umbel is longer than half of the total length; and L. gilchristi has choanosomal hexactins and the umbel diameter in the mesamphidiscs is proportionally larger. Lophophysema eversa sp. nov. differs from all three described species by its unusual body shape with basalia on the side of the body and by lacking macramphidiscs. Specifically, the new species (1) can be differentiated from L. australicum by lacking rough monactin; (2) can be distinguished from L. gilchristi in that the pinular ray of its atrialia is longer than that of dermalia and canalaria; and (3) is distinct from L. inflatum by having spined choanosomal hexactins..

Molecular data. The phylogenies of ML and BI analyses were highly congruent. The phylogeny based on 16S rRNA ( Figure 4 ) shows that L. eversa forms a clade with Hyalonema to the exclusion of the pheronematids, thus supporting the family assignment, with support values>70 for both the ML and BI analyses.

Figures

FIGURE 1. Lophophysema eversa sp. nov. holotype. A, a photograph showing its natural growth form on the seabed before it was collected by the mechanical hand of the manned submersible “ Jiaolong ”. B – C, external morphology of the specimen after being lifted out of seawater.

FIGURE 2. Diactins and acanthophores of Lophophysema eversa sp. nov. A – M, Diactins: A – E, general appearance of whole diactins. F – I, magnified ends of different types of diactins. J – M, magnified middle parts of different types of diactins. N – R, acanthophores: N, diactins, O – P, stauractins, Q, pentactins, R, hexactins.

FIGURE 3. Images of spicules of Lophophysema eversa sp. nov. A – I, SEM images of spicules. A – B, hypodermal pentactin. C, dermal pentactin. D, canalarial pentactin. E, atrial pentactin. F, hypoatrial pentactin. G, mesamphidisc. H, micramphidiscs. I, microhexactin. J – K, LM images of spicules. J, choanosomal hexactins. K, microhexactin.

FIGURE 4. Bayesian inference tree based on 16 S rRNA sequence data with the GTR + G substitution model; Bayesian posterior probabilities (left) and ML analysis bootstrap values (right) are shown on each branch. The scale bar corresponds to 0.08 expected substitutions per site.