Sporidesmiella lignicola X.H. Li, H.Y. Song & D.M. Hu, 2021

Li, Xiao-Hong, Liu, Yu-Lin, Song, Hai-Yan, Hu, Dian-Ming, Gao, Yang, Hu, Hai-Jing & Zhou, Jian-Ping, 2021, Sporidesmiella lignicola sp. nov., a new hyphomycetous fungus from freshwater habitats in China, Biodiversity Data Journal 9, pp. 77414-77414 : 77414

Sporidesmiella lignicola X.H. Li, H.Y. Song & D.M. Hu sp. nov.

Materials

Type status: Holotype. Occurrence: recordedBy: Xiao-Hong Li; individualCount: 1; Taxon: taxonID: urn:lsid:biosci.ohio-state.edu:osuc_names:275502; scientificName: Sporidesmiella lignicola; acceptedNameUsage: Sporidesmiella lignicola X.H. Li, H.Y. Song & D.M. Hu, 2021, sp. nov.; parentNameUsage: Sporidesmiella P.M. Kirk 1982; kingdom: Fungi; phylum: Ascomycota; class: Dothideomycetes; order: Pleosporales; family: Junewangiaceae; genus: Sporidesmiella; specificEpithet: lignicola; taxonRank: species; verbatimTaxonRank: species; scientificNameAuthorship: X.H. Li, H.Y. Song & D.M. Hu; Location: continent: Asia; country: China; stateProvince: Jiangxi; county: Xinfeng; municipality: Ji'an; locality: Jinji Forest Farm ; verbatimElevation: 305 m; locationRemarks: label transliteration: " Jiangxi, Jinji Forest Farm, 2020.7.7, Li Xiao-Hong"; [江西赣州市信丰县金鸡林场，2020年7月7日，李小红]; verbatimCoordinates: 25.4732 N, 115.2048 E; decimalLatitude: 25.4732; decimalLongitude: 115.2048; georeferenceProtocol: label; Identification: identifiedBy: Xiao-Hong Li and Dian-Ming Hu ; dateIdentified: 2020; Event : samplingProtocol: collecting; eventDate: 07/07/2021; habitat: Freshwater ; Record Level : type: PhysicalObject ; language: en; rightsHolder: Dian-Ming Hu ; institutionID: HFJAU 10001 ( Dried culture with conidia); collectionID: FF019; institutionCode: the Herbarium of Fungi , Jiangxi Agricultural University (HFJAU); collectionCode: Fungi ; ownerInstitutionCode: the Herbarium of Fungi , Jiangxi Agricultural University (HFJAU); basisOfRecord: PreservedSpecimen

Description

Saprobic on decaying wood submerged in freshwater habitats. Colonies effuse, hairy, pale brown. Mycelium mostly superficial, partly immersed, consisting of unbranched, septate, smooth, thick-walled, brown to dark brown hyphae. Sexual morph: Undetermined. Asexual morph: Conidiophores 110-150 × 3-7 μm (mean = 124.6 × 4.2, n = 20), macronematous, mononematous, solitary, pale brown, smooth at the bottom and verrucose at the apex, septate, unbranched, straight or slightly flexuous. Conidiogenous cells 15-26 × 2-5 μm (mean = 22.4 × 4, n = 20), holoblastic, polyblastic, integrated, terminal, pale brown, cylindrical. Conidia 18-26 × 7-11 μm (mean = 21 × 8.9, n = 20), acrogenous, dry, cylindrical, narrowly clavate, obovoid to broadly obovoid to cuneiform, truncate at the base, rounded or rarely coronate at the apex, 2-3-distoseptate, pale olivaceous to olivaceous brown or brown, smooth. Conidial session schizolytic (Fig. 1 ).

Culture characteristics: On PDA, colony reaching 12 mm in 21 days at 28°C, pink from above, pink-grey from below, surface rough, dry, with loose mycelium and irregular edge. After half a year, the colony produces spores. The hyphae penetrate into the PDA medium, the surface colour becomes brown to dark brown, raised with white in the middle, reverse of culture pale brown to dark brown, with entire and regular edge. Mycelium composed of septate, pale brown, unbranched, smooth hyphae. Conidiophores macronematous, solitary, cylindrical, straight or slightly flexuous, septate, brown, smooth, thick-walled, 37-54 × 3.5-5.5 μm (mean = 46.5 × 4.6, n = 20). Conidiogenous cells holoblastic, polyblastic, integrated, terminal, pale brown, cylindrical, 10-26 × 3-7 μm (mean = 27.1 × 4.6, n = 20), slightly enlarged towards the apex. Conidia acrogenous, cylindrical, broadly obovoid to cuneiform, truncate at the base, rounded at the apex, 3-4-distoseptate, brown to pale olivaceous brown, smooth, 18-28 × 8-12 μm (mean = 22.3 × 9.6, n = 20) (Fig. 2 ).

Etymology

The specific epithet " Sporidesmiella lignicola " (Latin) meaning ‘‘ growing on wood’’.

Ecology

Saprophyte on wood submerged in a small stream.

Notes

Sporidesmiella lignicola is characterised by being cylindrical, broadly obovoid to cuneiform, truncate at the base, rounded at the apex, 3-4-distoseptate, pale olivaceous brown to brown, smooth, which is consistent with the characteristics of Sporidesmiella . Sporidesmiella lignicola is similar to S. obovoidia and S. hyalosperma in having polyblastic conidiogenous cells and obovoid, 3-4-distoseptate, brown conidia ( Luo et al. 2019, Dong et al. 2021). However, S. lignicola differs from other species in having longer and verrucose conidiophores (Table 2 ). In addition, the colonies of S. lignicola are pink from above, pink-grey from below, characteristics which were not observed in the other two species (Fig. 3 , Table 2 ).

Based on a BLAST of NCBI’s GenBank nucleotide database, the most similar sequence was Sporidesmiella obovoidia . The nucleotide comparison between S. lignicola and S. obovoidia showed differences of 10 and 4 nucleotides in ITS and LSU sequence data, respectively (Fig. 4 , Fig. 5 ), which supported them to be different species ( Jeewon and Hyde 2016).

Unfortunately, the strain could not be successfully activated due to improper operation during preservation. When the original culture was retained for 6 months, the sporulation of mycelium could be observed under the microscope (Fig. 2 ). We deposited the dried culture as specimens (HFJAU 10001) of this species.

Figures

Figure 1. Sporidesmiella lignicola (HFJAU 10001, Holotype) a Colony on wood; b, c Conidiophores; d Conidiophores with production of conidia; e - g Conidia; h, i Colony on PDA for 21 days (left-front, right-reverse). Scale bars: a = 125 µm, b-c = 12.5 µm, d-g = 10 µm, h-i = 1.5 cm.

Figure 2. a-b, e Conidiophores with production of conidia; c-d Conidiophores; f-i Conidia; j Colony on PDA after 6 months (left-front, right-reverse). Scale bars: a-d = 25 µm, e-i = 10 µm, d-g = 10 µm.

Figure 3. Comparisons of colonies on PDA (left-front, right-reverse) in Sporidesmiella lignicola and similar species. a S. hyalosperma; b S. obovoidia; c S. lignicola.

Figure 4. The specific base differences between S. lignicola and S. obovoidia in ITS. Different base pairs have been marked on specific sites, and the same base is omitted.

Figure 5. The specific base differences between S. lignicola and S. obovoidia in LSU. Different base pairs have been marked on specific sites, and the same base is omitted.