Ascodesmis rosicola H. Zhang & Y. L. Jiang, 2021

Zhang, Hong, Wei, Tian-Peng, Mao, Yu-Tao, Ma, Ming-Xia, Ma, Kai, Shen, Ying, Zheng, Mei-Juan, Jia, Wei-Yu, Luo, Ming-Yan, Zeng, Yan, Jiang, Yu-Lan & Tao, Guang-Can, 2021, Ascodesmis rosicola sp. nov. and Talaromyces rosarhiza sp. nov., two endophytes from Rosa roxburghii in China, Biodiversity Data Journal 9, pp. 70088-70088 : 70088

Ascodesmis rosicola H. Zhang & Y. L. Jiang sp. nov.

Materials

Type status: Holotype. Occurrence: catalogNumber: GUCC 190035.1 ; recordedBy: Hong Zhang; Taxon : scientificName: Ascodesmis rosicola; kingdom: Fungi ; phylum: Ascomycota ; class: Pezizomycetes ; order: Pezizales ; family: Ascodesmidaceae ; genus: Ascodesmis ; Location : country: China; stateProvince: Guizhou; locality: Guiyang ; Identification : identifiedBy: Hong Zhang ; dateIdentified: 2021 Type status: Other material. Occurrence: catalogNumber: GUCC 190204.1 ; recordedBy: Hong Zhang; Taxon : scientificName: Ascodesmis rosicola; kingdom: Fungi ; phylum: Ascomycota ; class: Pezizomycetes ; order: Pezizales ; family: Ascodesmidaceae ; genus: Ascodesmis ; Location : country: China; stateProvince: Guizhou; locality: Guiyang ; Identification : identifiedBy: Hong Zhang ; dateIdentified: 2021

Description

Endophytic fungi of R. roxburghii . Asexual morph not observed. Sexual morph (Fig. 1 ): Apothecia gregarious, superficial, sessile, 200-320 µm diameter, first hyaline, becoming brownish upon maturity. Excipulum absent. Asci broadly clavate or obovoid, with a short broad stalk or a broad base, 30.5-50.0 × 12.5-21.0 µm (av. = 38.0 × 19.0 µm, n = 30), 6-8-spored. Ascospores irregularly arranged, ellipsoid to perfectly spherical (length/breadth ratio 1.01-1.69, av. 1.32), at first hyaline, brownish upon maturity, 5.0-13.0 × 4.5-10.0 µm (av. = 10.5 × 8.0 µm, n = 30), at first smooth, later ornamented with a very variable pattern of isolated warts and spines, occasionally with a few fine connectives and one or two very obvious simple or branched ridges extending over the major part of the ascospore surface, very few without ridge. Paraphyses septate, simple and hyaline, 3.5-6.5 µm thick.

Cultural characteristics: Description based on GUCC 190035.1. On PDA, reaching 6.0-7.5 cm in diameter after 14 days of cultivation in dark at 28°C, superficial, margin irregular, white, with abundant aerial mycelium, uneven, centre and margin uplifted; reverse yellowish. On MEA, 28°C: medium sparse, circular, flat. Mycelium white, cottony, margin regular. On OA, 28°C: radial, aerial mycelium sparse, pale white, growth regular. On MEA and OA, reaching 85 mm in diameter under the same conditions.

Etymology

The name refers to the host plant, Rosa roxburghii , from which this fungus was isolated.

Notes

Ascodesmis rosicola is introduced as a new species, based on morphological characteristic and phylogenetic analysis. The genus Ascodesmis , established by Tieghem (1876), presently contains 13 species ( Trivedi et al. 1973, Brummelen 1981, Currah 1986), but these species have no available sequence data, except A. microscopica , A. nigricans and A. sphaerospora . In the phylogenetic analyses (Fig. 2 ), using the combined ITS, LSU and RPB2 sequence data of Pezizales , A. rosicola is related to Ascodesmis spp. The two A. rosicola isolates clustered with good support (BS = 94, PP = 1) and placed in a distinct clade, albeit with moderate support (BS = 61, PP = 0.92). This species shows some similarity to A. porcina in having ascospores with a conspicuously simple or branched ridge extending over the most part of the spore surface, which is well separated from other Ascodesmis spp. However, A. rosicola differs from A. porcin by its smaller asci (30.5-50.0 × 12.5-21.0 µm vs. 65-80 (-90) × 20-30 (-35) µm) and smooth surface of young ascospores (those of A. porcin are ornamented) ( Brummelen 1981). Moreover, the hosts of A. rosicola and A. porcin (from the dung of donkey, goat, peccary, pig and rat) ( Brummelen 1981) were notably different.

Figures

Figure 1. Ascodesmis rosicola (GUCC 190035.1, holotype). A-F On PDA, MEA and OA, respectively in 14 days at 28 ° C (A, C and E from above, B, D and F from below); G Sexual morph on SNA; H Paraphyses; I-K Apothecium and asci; L-Q Ascospores. Scale bars: G = 0.5 mm, H-K = 20 μm, L-Q = 10 μm.

Figure 2. Phylogram generated from Maximum Likelihood analysis, based on combined ITS, LSU and RPB 2 sequence data. Geopyxis vulcanalis (KH. 04.37 (FH, dupl. DBG )) is used as the outgroup taxa. Bootstrap support values for ML greater than 50 % and Bayesian posterior probabilities greater than 0.90 are given near nodes (BS / PP), respectively. The novel taxon is indicated in bold and black. T: type or ex-type.