Phylloporia elegans Ferreira-Lopes, Robledo & Drechsler-Santos, 2016

Luciorobledo, Gerardo, Reck, Mateusarduvino, Góes-Neto, Aristóteles & Drechsler-Santos, Elisandro Ricardo, 2016, Phylloporia spathulata sensu stricto and two new South American stipitate species of Phylloporia (Hymenochaetaceae), Phytotaxa 257 (2), pp. 133-148 : 140-141

Phylloporia elegans Ferreira-Lopes, Robledo & Drechsler-Santos sp.nov. ( Figures 2 B , 4 A–E )

Mycobank:—MB 805726

Diagnosis:—Basidiomata seasonal to reviving, stipitate, dark yellowish brown pileus covered by short plagiotrichoderm, 8–12(–13) pores/mm and basidiospores broadly ellipsoid to ellipsoid (2.5–3.5 x 2–3 μm).

Etymology:— Elegans (Lat.) : referring to its slender stipe and delicate basidioma.

Holotype:— BRAZIL. Santa Catarina: Itapoá, Reserva Particular do Patrimônio Nacional Volta Velha , elevation 20 m. a. s. l., – 26º 04’56” S, – 48º 38’ 56” W, on living roots of a undetermined angiosperm, 18 th November 2012, Robledo 2545 ( FLOR 51178 ). GenBank accession number ( LSU; KJ631408 ).

Description:—Basidiomata seasonal to reviving, stipitate, solitary to laterally fused at the base of the stipe; pileus circular to semicircular, spathulate to flabeliform, infundibiliform, up to 1.5 cm wide by 1.5 mm thick; pileus surface a short plagiotrichoderm, shiny, adpressed velutinate to hispid, concentrically zonate and sulcate, sometimes slightly radially wrinkled, dark yellowish brown (10YR 4/4–4/6) at the center, becoming light olive brown (2.5Y 5/4–5/6) towards the margin, in section appearing light olive brown (2.5Y 5/4–5/6), up to 0.5 mm thick, separated from the context by a resinous and very thin dark line (up to 0.1 mm); context compact, slightly lighter in color than the superior portion, up to 0.5 mm thick; tube layer concolorous with the context, up to 0.5 mm, indistinctly stratified when reviving; margin rounded, entire to lobed, 1–2 mm thick, whitish when fresh, becoming yellowish (2.5Y 7/6–7/8) upon drying; hymenophore slightly decurrent, yellowish (2.5Y 7/6) to light olive brown (2.5Y 5/4–5/6), pores inconspicuous, circular to angular, 8–12(–13)/mm, when reviving, with spots of new tube layers growing irregularly upon older parts, dissepiment entire. Stipe attached to living roots, mostly standing straight, eccentric to lateral, bulbose to cylindrical, uneven and appearing knotted, up to 1 cm wide at the base, tapering towards the pileus insertion point, up to 3.0 cm high; stipe surface an appressed coat of tomentum, dull, dark yellowish brown (10YR 4/4–4/6), up to 1 mm thick, a thin dark line separating it from the context; stipe context compact, shiny, the same as the context of the pileus in color and texture, up to 4 mm wide.

Hyphal structure monomitic in all parts (truly monomitic sensu Corner 1991). Generative hyphae simple-septate, slightly to moderately thick-walled, with wide lumen, sometimes with portions filled with a bright yellowish resin, pale to golden yellow, more frequent in the plagiotrichoderm; at the hymenophoral trama (2–)2.5–4 μm wide; at the context of pileus and stipe subparallel, (3–)4–6 μm wide; at the plagiotrichoderm ( Figure 4 A ) often collapsed and with local constrictions towards the apices, 3–5(–6) μm wide, up to 300 μm length, anticlinal, arising from a dark line formed by sclerified brownish agglutinated hyphae, frequently septate and branched; at the stipe tomentum loosely interwoven, mostly unbranched, straight to slightly tortuous, 2–4 μm wide. Hymenium ( Figure 4 E ). Basidia 4-sterigmate, clavate to barrel-shaped, hyaline in KOH, 7–8 × 4–5 μm, basidioles shorter. Cystidia rarely present, subulate, hyaline in KOH, thin-walled, 12–13 × 3–4(–5) μm. Basidiospores ( Figure 4 B–D ) broadly ellipsoid to ellipsoid in equatorial lateral view, subglobose in equatorial adaxial view, sometimes with either one flattened side on the equatorial or in the polar view, or in both; smooth; pale yellow in KOH/lactophenol; occasionally guttulate; thick-walled; 2.5–3.5 × 2–3 μm (avg. = 3.0 × 2.4 μm), Q = 1.50–1.75/Q avg.= 1.28, thick-walled, IKI-.

Substrate and distribution:—Growing on living angiosperm roots; some specimens were connected to living roots of Eugenia involucrata D. C ( Myrtaceae Juss. ). So far known from Brazilian Atlantic Forest Biogeographic Province (Santa Catarina State) and Parana Forest Biogeographic Province (northeast Argentina).

Additional specimens examined:— ARGENTINA. Province of Misiones: Department Oberá, Centro de Investigaciones Antonia Ramos , elevation 400 m. a. s. l., 27° 19’ 58.80” S, 54° 57’ 0.00” W, growing on living roots of Eugenia involucrata D. C., 24 th February 2015, V. F. Lopes 102 ( CORD!) ; Idem, Ibidem , 27 th February 2015, V. F.Lopes 106, growing on living roots of E. involucrata ; BRAZIL. Santa Catarina: Itapoá, Reserva Particular do Patrimônio Nacional Volta Velha , elevation 20 m. a. s. l., 26º 04’ 56 S, 48º 38’ 56” W, growing on living roots, 18 th November 2012, Robledo 2546 ( FLOR! 51179) ; Idem, Ibidem , 28 th February 2012, E. R. Drechsler-Santos 790 ( FLOR! 51169) ; Idem, Ibidem , 28 th February 2012, E. R. Drechsler-Santos 792 ( FLOR! 51238) .

Figures

FIGURE 2. A) P. spathulata (Holotype); B) P. elegans (Holotype); C) P. nodostipitata (FLOR 51154), on the upper-right corner, detail of P. nodostipitata pileus and stipe in section. Photographs by Gerardo L. Robledo (A, B) & Valéria F. Lopes (C).

FIGURE 4. Phylloporia elegans microscopic features. A) pileus section with black arrow indicating the plagiotrichoderm; B–D) Sporogram from light microscope observations of fresh specimens (FLOR 51179, FLOR 51178), representing three variations of the basidiospores in equatorial and polar view, with dotted lines representing flattened sides; E) Basidia, basidiole and subulate cystidia, mature and young (el = equatorial lateral view; ead = equatorial adaxial view; eab = equatorial abaxial view; dp = distal polar view and pp = proximal polar view). Drawings by Gerardo L. Robledo & Valéria Ferreira Lopes.