Apis mellifera, Linnaeus, 1758

Negiş, İnci Şahin, Kibar, Mustafa & Aytekin, İbrahim, 2024, The association between ClaI polymorphism and hygienic behavior in Apis mellifera, Turkish Journal of Zoology 48, pp. 47-53 : 47

3.1. PKB assay in A. mellifera

In the present study, as mentioned earlier, the PKB assay was performed twice in April and the results were recorded. The percentage of cleaned pupae cells was scored for hygienic behavior. As a result of the PKB test, the mean values for the hygiene behavior of the colonies were between 68% and 100%. Accordingly, the 6 colonies (Italian hybrid (100%), Pure Anatolian (99%), Carniolan hybrid (99%), Carpathian hybrid (98.5%), and Caucasian hybrid (96.5%)) showed hygienic behavior ( Table 2).

3.2. ClaI polymorphism of SNP-8 gene in A. mellifera After the PKB assay, 148 bees were sampled for molecular assay. The SNP-8 primer, PCR, and restriction enzyme conditions used in the study conducted by Kim et al. (2019) were taken into consideration. A 392 bp region of the SNP-8 gene region was amplified. The polymorphisms were observed by digesting the PCR products with the