Pulmonaria hirta L. (Boraginaceae)

Astuti, Giovanni, Liu, Lijuan & Peruzzi, Lorenzo, 2019, Chromosome numbers for the Italian flora: 7, Italian Botanist 7, pp. 183-187 : 183

Pulmonaria hirta L. (Boraginaceae)

Chromosome number.

2n = 28 (Figs 4, 5, 6)

Voucher specimen.

ITALY. Toscana. Poppi (Arezzo), all’Eremo di Camaldoli (WGS84: 43.80902N, 11.82518E), lungo la SP124 nel tratto che porta dall’eremo al Prato alla Penna, al margine della faggeta, 1150 m s.l.m., 24 April 2018, G. Astuti et L. Liu (PI n° 021358-021376); Santa Maria a Monte (Pisa), Valle Lupitana (WGS84: 43.71388N, 10.67218E), nelle schiarite del bosco, 39 m s.l.m., 21 March 2018 G. Astuti et L. Liu (PI n° 021343-021357); Emilia-Romagna. Grizzana Morandi (Bologna), lungo la SP73 presso la località Favari, (WGS84: 44.22385N, 11.09556E), a margine del querceto, 539 m s.l.m., 19 April 2018, G. Astuti et L. Liu (PI n° 021377-021396).

Method.

Squash preparations were made on root-tips obtained from potted plants. Root tips were pre-treated with 0.4% colchicine for 3 hours and then fixed in Carnoy fixative solution for 1 hour. After hydrolysis in HCl 1N at 60 °C, the tips were stained in leuco-basic fuchsine.

Observations.

A specimen collected in Camaldoli (Toscana) was selected as the epitype for the name P. hirta by Selvi in Cafferty and Jarvis (2004). We found 2n = 28 chromosomes, the chromosome number typical of this species ( Cecchi and Selvi 2015), and no variation among the 16 individuals sampled in this topotypical population. A specimen coming from Valle Lupitana (Toscana) was selected by Puppi and Cristofolini (1996) as the neotype for the name Pulmonaria picta Rouy, a heterotypic syonym of P. hirta . Also for the 18 individuals sampled in this population, we found 2n = 28 chromosomes, in agreement with other counts from the same geographical area ( Vosa and Pistolesi 2004). The identification of the population from Favari (Emilia-Romagna) was based on morphological features of basal leaves, and the 18 individuals sampled, all showing 2n = 28 chromosomes, confirmed our identification.

G. Astuti, L. Liu, L. Peruzzi