Nannizzia sinensis Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang, 2023

Zhang, Zhi-Yuan, Li, Xin, Chen, Wan-Hao, Liang, Jian-Dong & Han, Yan-Feng, 2023, Culturable fungi from urban soils in China II, with the description of 18 novel species in Ascomycota (Dothideomycetes, Eurotiomycetes, Leotiomycetes and Sordariomycetes), MycoKeys 98, pp. 167-220 : 167

Nannizzia sinensis Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang sp. nov.

Fig. 13

Etymology.

Refers to the country where this fungus was first isolated.

Type.

China: Guizhou Province, Zunyi City, the affiliated hospital of Zunyi Medical University 27°70'79"N, 106°94'54"E, soil, 5 Jul 2016, Z.Y. Zhang (HMAS 351892 holotype designated here, ex-type living culture CGMCC 3.20873 = GZUIFR 22.012) .

Description.

Culture characteristics (14 d at 25 °C): Colony on PDA 76-79 mm diam., yellowishwhite (4A2), floccose, fluffy, edge entire to diffuse; reverse white (4A1). Colony on MEA 51-52 mm diam., yellowish-white (4A2), floccose, wavy from centre to margin, edge entire; reverse white (6A1) to brownish-orange (6C8) from margin to centre. Colony on OA 52-54 mm diam., white (1A1), fluffy, sparse at the centre, margin irregular; reverse white (1A1).

Hyphae hyaline, septate, smooth, branched, 1.0-4.5 μm wide; racquet hyphae and spiral hyphae not observed. Macroconidia abundant, thin- or moderately thick-walled, smooth-walled or slightly verrucose, borne individually on short branches alongside hyphae or complex branched conidiophores, fusiform, 1-5-septate, 45.0-51.0 × 11.5-12.5 μm (av. 48.6 × 12.3 μm, n = 50). Microconidia scant, sessile or short-stalked, aseptate, smooth-walled. Two types of microconidia are present: subspherical to spherical, 5.0-9.0 × 5.0-8.0 μm (av. 8.2 × 6.8 μm, n = 50); obovoidal or clavate, 3.5-6.5 × 2.0-2.5 μm (av. 5.4 × 2.3 μm, n = 50). Sexual morph not observed.

Additional specimens examined.

China: Hainan Province, Haikou City, Haidian Campus of Hainan University 20°05'76"N, 110°32'91"E, soil, 28 Aug 2019, Z.Y. Zhang, GZUIFR 22.054 ; Jiangxi Province, Jian City, Jinggangshan University , soil, 22 Aug 2019, Z.Y. Zhang, GZUIFR 22.055, ibid., GZUIFR 22.056 .

Notes.

In the multi-locus phylogenetic analysis, our four isolates form a distinct clade and are closely related to N. aenigmatica , N. gypsea and N. lorica (Fig. 12 ). However, N. sinensis can be distinguished from N. aenigmatica by the presence of macroconidia ( Dukik et al. 2020). In addition, N. sinensis differs from N. gypsea and N. lorica by the presence of subspherical to spherical microconidia ( Dukik et al. 2020).

Figures

Figure 12. Concatenated phylogeny of the ITS, LSU, TUB, TEF 3 and RP 60 S L 1 gene regions of species in Arthrodermataceae. Three hundred and eighteen strains are used. The tree topology of the BI was similar to the ML analysis. Bayesian posterior probability (≥ 0.7) and ML bootstrap values (≥ 70 %) are indicated along branches (PP / ML). Novel species are in blue and bold font, new isolates are in blue and " T " indicates type derived sequences.

Figure 13. Nannizzia sinensis (from ex-holotype CGMCC 3.20873) a-c upper and reverse views of cultures on PDA, MEA and OA 14 d after inoculation d subspherical to spherical microconidia e-h conidiophores with sessile or stalked microconidia i-k conidiophores and macroconidia l macroconidia. Scale bars: 10 µm (d-l).