Tupiella speciosa Darienko and Pröschold, 2017

Kwon, Dae Ryul, Nam, Hyun Ju & Nam, Bok Yeon Jo and Seung Won, 2022, New records of two ulvophycean freshwater species, Lithotrichon pulchrum (Ulvales, Ulvophyceae) and Tupiella speciosa (Ulotrichales, Ulvophyceae), Journal of Species Research 11 (2), pp. 79-88 : 84

2) Tupiella speciosa Darienko and Pröschold, 2017

Material examined. Sample was collected from the Songchonji Pond Sangchon-ri , Nakdong-myeon, Sangjusi, Gyeongsangbuk-do, Republic of Korea (36°22′11.5″N, 128°14′42.3″E) on 26 February 2018 .

Description. Tupiella speciosa is a microscopic and filamentous alga. Vegetative cells were round-shaped ( Fig. 3A ). Cells possess a nucleus, Golgi body, mitochondria with plate-like cristae, and a single parietal chloroplast with a pyrenoid penetrated by a thylakoid membrane ( Fig. 3A , 4A- D ). The pyrenoid matrix was penetrated by a single thylakoid membrane ( Fig. 4B ). Young filament cells had a chloroplast with 1-4 pyrenoids encased by starches ( Fig. 4F, G ). The terminal cells are slightly pointed ( Fig. 3B ). Akinetes are oval-shaped and enveloped by the thick cell wall ( Fig. 3C ). Akinete cell divided into four ( Fig. 3D ). In the akinetes cell, many starches were developed between thylakoid membranes ( Fig. 4E ).

Distribution. Czech Republic ( Darienko and Pröschold, 2017) and Republic of Korea.

Voucher slides. Two slides of gelatin-embedded specimens (NNIBRCL16652 and NNIBRCL16653) were deposited at Nakdonggang National Institute of Biological Resources, Korea.

Remarks. Tupiella speciosa cells were unicellular form in liquid medium. However, a young filament form was observed on an agar plate culture ( Darienko and Pröschold, 2017). Also, mature cultures produce many swollen cells, which is growing thin filaments that become branched or produce 2-4-cell packages, but matured plant form (mature thallus) is not observed in this study ( Darienko and Pröschold, 2017).

Figures

Fig. 3. Morphology of Tupiella speciosa Songchonmot180226S.A. Vegetative cell; B. Young filament; C, D. Thick cell wall-enveloped aki- netes including 1-4 cells. CW, cell wall; Py, pyrenoid. Scale bars: 10μm.

Fig. 4. Ultrastructure of Tupiella speciosa. A. Vegetative cell; B. Pyrenoid penetrated by thylakoid membrane(arrow); C. Mitochondrion; D. Golgi body; E. Akinete including two cells; F. Flament cell with one pyrenoid; G. Flament cell with four pyrenoids. Cp, chloroplast; CW, cell wall; G, Golgi body; Mt, mitochondrion; N, nucleus; Py, pyrenoid; S, starch. Scale bars: A, E-G=2 μm, B=1 μm, C, D=0.5 μm.