Spathaspora parajiuxiensis C.Y. Chai & F.L. Hui, 2020

Lv, Shi-Long, Chai, Chun-Yue, Wang, Yun, Yan, Zhen-Li & Hui, Feng-Li, 2020, Five new additions to the genus Spathaspora (Saccharomycetales, Debaryomycetaceae) from southwest China, MycoKeys 75, pp. 31-49 : 31

Spathaspora parajiuxiensis C.Y. Chai & F.L. Hui sp. nov. 836447 Figure 5

Type.

China, Yunnan Province, Honghe Prefecture, Luxi County, in rotting wood in Jiuxi Mountain Forest Park, July 2016, R.C. Ren & L. Zhang (holotype, NYNU 16747T preserved in a metabolically-inactive state), ex-holotype: CICC 33162; CBS 14691.

Etymology.

Paraluxiensis refers to its close phylogenetic relationship to Sp. luxiensis .

Description.

In YM broth after 3 days at 25 °C, cells are ovoid to elongate (3.5-4 × 7-15 μm) and occur singly or in pairs (Fig. 5a ); pseudohyphae are present. Budding is multilateral. Sediment is formed after a month, but a pellicle is not observed. After 3 days of growth on YM agar at 25 °C, colonies are white to cream-coloured, butyrous and smooth with entire margins. After 12 days at 25 °C on Dalmau plate culture on CM agar, pseudohyphae and true hyphae are formed (Fig. 5b ). Sporulation occurs on 5% ME agar after 14 days at 25 °C. Unconjugated asci are formed from single cells with one elongated ascospores which are tapered and curved at the ends (Fig. 5c ) Glucose and maltose are weakly fermented. Xylose fermentation is negative using Durham tubes, but ethanol is produced from xylose when determined with alcohol oxidase and peroxidase tests. Glucose, d-glucosamine, d-ribose, d-xylose, sucrose, maltose, trehalose, methyl α-d-glucoside, cellobiose, salicin, arbutin, melezitose, inulin, ribitol, d-glucitol, d-mannitol, 2-keto-d-gluconate, succinate and ethanol are assimilated. No growth occurs with galactose, l-sorbose, l-arabinose, d-arabinose, l-rhamnose, melibiose, lactose, raffinose, glycerol, erythritol, xylitol, galactitol, myo -inositol, d-glucono-1, 5-lactone, 5-keto-d-gluconate, d-gluconate, d-glucuronate, dl-lactate, citrate, l-arabinitol or methanol. For the assimilation of nitrogen compounds, growth on l-lysine, glucosamine or d-tryptophan is present, whereas growth on nitrate, nitrite, ethylamine, cadaverine, creatine, creatinine or imidazole is absent. Growth is observed at 37 °C, but not at 40 °C. Growth in the presence of 0.01% cycloheximide is present, but growth in the presence of 0.1% cycloheximide, 10% NaCl plus 5% glucose and 1% acetic acid is absent. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative.

Additional isolate examined.

China, Yunnan Province, Honghe Prefecture, Luxi County, in rotting wood in Jiuxi Mountain Forest Park, July 2016, R.C. Ren & L. Zhang, NYNU 16632.

Figures

Figure 5. Morphology of Spathaspora parajiuxiensis (NYNU 16747, holotype) a budding cells and pseudohyphae on YM broth after 3 d b true hyphae with blastoconidia on CM agar after 14 d c ascus and ascospore (arrow) on 5 % ME agar after 14 d. Scale bars: 10 μm.