Nagrajomyces fusiformis C. L. Hou & L. Zhuo, 2022

Zhuo, Lan, Guo, Mei-Jun, Wang, Qiu-Tong, Zhou, Hao, Piepenbring, Meike & Hou, Cheng-Lin, 2022, A new study of Nagrajomyces: with two new species proposed and taxonomic status inferred by phylogenetic methods, MycoKeys 93, pp. 131-148 : 131

Nagrajomyces fusiformis C. L. Hou & L. Zhuo sp. nov.

Figs 2 , 3

Etymology.

The epithet Nagrajomyces fusiformis refers to fusoid conidia.

Type.

China, Yunnan province, Lijiang, Yulong , 26°40'55"N, 99°54'01"E, alt. 2762 m, on dying twigs of Rhododendron vellereum Hutch. ex Tagg., 20 June 2021, coll. C.L. Hou, M.J. Guo, H. Zhou (holotype CAF 800050) .

Diagnosis.

This new species differs from N. dictyosporus and N. laojunshanensis by fusoid to elongate-fusoid conidia with pointed ends, usually 1-septate and smaller.

Description.

Conidiomata solitary, pycnidial, irregularly plurilocular, subepidermal in origin, immersed at first, then becoming erumpent through the periderm of the host, 545-554 μm diameter, 520-546 μm high, peridium dark brown, 47.0-67.5 μm thick. Conidiophores ampulliform, smooth, hyaline, multiguttulate, 12-29 × 2.0-3.5 μm (x̄ = 19 × 3 μm, n = 20). Conidia fusoid to elongate-fusoid, 1-septate, cells equal, smooth, hyaline to pale brown, 13.5-19.0 × 3-4 μm (x̄ = 16.5 × 3.5 μm, n = 20), with a whip-like appendage at the tip of each conidium, 30-77 μm (x̄ = 51 μm, n = 20) in length (Fig. 4 ). Sexual morph not observed.

Additional specimen examined.

China, Yunnan Province, Lijiang, Laojunshan, 26°37'56"N, 99°43'30"E, alt. 3873 m, on dying twigs of Rhododendron vellereum , 20 June 2021, coll. C.L. Hou, M.J. Guo, H. Zhou (BJTC 1773).

Notes.

Nagragomyces fusiformis differs from other species of Nagrajomyces by narrower and 1-septate conidia.

Figures

Figure 2. Micrographs of Nagrajomyces fusiformis (holotype CAF 800050) on twigs of Rhododendron vellereum A, B conidiomata on a dying twig C vertical section of a conidioma D conidiophores and conidia at diverse developmental stages E-H conidia with appendages. Scale bars: 2 mm (A); 200 µm (B); 100 µm (C); 10 µm (D-H).

Figure 3. Nagrajomyces fusiformis (holotype CAF 800050) A vertical section of a conidioma B conidiophores and conidia C conidia with appendages. Scale bars: 100 µm (A); 10 µm (B); 5 µm (C).

Figure 4. Micrographs of Nagrajomyces laojunshanensis on Rhododendron cinnabarinum (holotype CAF 800049) A conidiomata on a living twig B ex-type culture (CFCC 58177) on PDA after 30 days, seen from above C ex-type culture (CFCC 58177) on MEA after 30 days, seen from above D vertical section of a conidioma E, F conidiophores and conidia G conidia forming a cirrus H conidium with appendage. Scale bars: 1 mm (A); 1 cm (B, C); 100 µm (D, E); 10 µm (F-H).