Samsoniella haniana Hong Yu bis, Yao Wang & Z.Q. Wang, in Wang, Wang, Dong, Fan, Dao & Yu, Journal of Fungi 8: 20, 2022

Chen, Wan-Hao, Liang, Jian-Dong, Ren, Xiu-Xiu, Zhao, Jie-Hong & Han, Yan-Feng, 2023, Two new species of Samsoniella (Cordycipitaceae, Hypocreales) from the Mayao River Valley, Guizhou, China, MycoKeys 99, pp. 209-226 : 209

Samsoniella haniana Hong Yu bis, Yao Wang & Z.Q. Wang, in Wang, Wang, Dong, Fan, Dao & Yu, Journal of Fungi 8: 20, 2022

Fig. 5

Description.

Synnemata arising from every part of the body of the pupa host. Synnemata erect, usually irregularly branched at the apex, Isaria -like morph producing a mass of conidia at the branch apex, powdery and floccose. Colonies on PDA, attaining a diameter of 32-35 mm after 14 days at 25 °C, white, consisting of a basal felt, floccose hyphal overgrowth; reverse yellowish. Hyphae septate, hyaline, smooth-walled, 1.3-1.8 μm wide. Conidiophores hyaline, smooth-walled, with single phialide or whorls of 2-8 phialides or verticillium-like from hyphae directly, 16.1-23.9 × 1.7-2.2 μm. Phialides consisting of a cylindrical to ellipsoidal, somewhat inflated base, 5.0-6.9 × 1.8-2.5 μm, tapering to a thin neck. Conidia hyaline, smooth-walled, fusiform to subglobose, 1.7-3.4 × 1.7-2.1 μm, forming divergent and basipetal chains. Sexual state not observed.

Host.

Pupa ( Lepidoptera ).

Material examined.

China, Guizhou, Qiannan Buyei and Miao Autonomous Prefecture, Duyun City , Mayao River Valley (26°22'8.3748"N, 107°23'16.96"E). On a pupa ( Lepidoptera ), buried in soil, 4 September 2021, Wanhao Chen, GZAC DY09103 (specimen), DY091031, DY091032 (living culture); On a ladybug ( Coccinellidae ); On the moss, 4 September 2021, Wanhao Chen, GZAC DY09102 (specimen), DY091021, DY091022 (living culture); On a caterpillar ( Lepidoptera ), buried in soil, 4 September 2021, Wanhao Chen, GZAC DY09115 (specimen), DY091151, DY091152 (living culture); On a pupa ( Lepidoptera ), buried in soil, 4 September 2021, Wanhao Chen, GZAC DY0929, DY09158 (specimen) .

Remarks.

Strains DY091021, DY091022, DY091031, DY091032, DY091151 and DY091152 were identified as belonging to Samsoniella , based on the BLASTn result and the phylogenetic analyses (Fig. 1 ) and clustered with S. haniana in a subclade with high statistical support (100% ML/ 1 PP). The characteristics of those strains were very closely linked with S. haniana , which had fusiform or oval conidia (2.3-3.7 × 1.2-2.8 μm), phialide (5.4-12.1 × 1.2-2.9 μm) and a pupa host. Thus, the molecular phylogenetic results and morphologically-based conclusions supported the idea that strains DY091021, DY091022, DY091031, DY091032, DY091151 and DY091152 were S. haniana .

Figures

Figure 1. Phylogenetic relationships amongst the new strains and their allies based on multigene dataset (ITS, LSU, RPB 1, RPB 2 and TEF). Statistical support values (≥ 70 % / 0.70) are shown at the nodes for ML bootstrap support / BI posterior probabilities. The new strains or species are in bold type.

Figure 5. Samsoniella haniana A infected pupa (Lepidoptera) B, C PDA culture plate showing top (B) and reverse (C) sides of the colony D-I phialides and conidia. Scale bars: 10 mm (B, C); 10 μm (D-I).