Eugenysa columbiana (Boheman)

Chaboo, Caroline S., 2002, First Report Of Immatures, Genitalia And Maternal Care In Eugenysa Columbiana (Boheman) (Coleoptera: Chrysomelidae: Cassidinae: Eugenysini), The Coleopterists Bulletin 56 (1), pp. 50-67 : 4-6

Eugenysa columbiana (Boheman)

Calaspidea columbiana Boheman, 1850:212 (original description); Boheman, 1856:35 (catalog); Boheman, 1862:91 (catalog); Gemminger and Harold, 1876:3629 (list); Wagener, 1877:53 (list); Spaeth, 1905:91 (description); Spaeth, 1914:31 (catalog); Spaeth, 1937:85 (description).

Calaspidea columbina: Champion, 1893:135 (localities; misspelling); Borowiec, 1999:79 (cited as misspelling).

Eugenysa columbiana: Blackwelder, 1946:737 (checklist); Viana, 1968:21 (description); Wilcox, 1975:150 (checklist); Windsor and Choe, 1994:113 (distribution); Borowiec, 1996:184 (list); Borowiec, 1999:79 (catalog).

Description. Male genitalia ( Fig. 3 ). Median lobe forming 90° angle with basal piece; ejaculatory guides present; tegmen with manubrium (= basal piece) as long as arms (= lateral lobes); lateral plates slightly projected from rim of apical foramen. Ejaculatory duct very long, about 8 times longer than aedeagus; junction of duct and seminal vesicle with sclerotized protuberant knob; seminal vesicle wide distally and tapered proximally, proximal portion deflexed and tapered into vas deferens; duct coiled, coils loose proximally and tight distally.

Female Genitalia Ovipositor. spiculum lightly sclerotized, with emarginate apex, and widened distally; palpi shorter than T8. Reproductive tract. Whitish; each ovary with 11–15 ovarioles; calyx, lateral oviducts and common oviduct enclosed in layer of stringlike tissue; bursa copulatrix rounded; oviduct section distal to bursa copulatrix with paired lateral lobes and short paired mesal projections; mesal projections yellowish. Spermatheca ( Figs. 4–5 ). Well­sclerotized, falcate, with muscle on inner margin; pump with diameter smaller than in receptacle, deflexed and apically narrowed; appendix present. Receptacle complex, with three chambers distinguishable externally. Gland present, elongate and basally positioned; spermathecal duct and gland entry into receptacle chambers clearly separated, with spermathecal duct entering chamber 1 and gland entering chamber 2. Duct very long, more than 15 times spermathecal length, entirely tightly coiled.

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Larva ( Figs. 6–12 ), 5th instar: length 10.8–14.0 mm; width at greatest diameter 6.4– 8.0 mm, (n = 10). Body color generally yellow, tergum greyish yellow, dorsum with faint yellow medial stripe; thoracic sterna whitish; head, pronotum, legs, scoli and caudal furcae brown. Shape ovate, widest across metathorax, dorso­ventrally compressed; 14 pairs of dorso­lateral scoli and 1 pair caudal furcae present. Dorsal surface densely microtrichiate; setae present, sparse medially, more dense in pleural area. Ventral surface densely microtrichiate; setae present; setae denser, longer and finer on medial surface of thoracic segments; sternal, tergal and pleural areas with short sparse setae. Spiracles annulate. Head: exposed in dorsal view, rounded; dorsal surface with sparse setae; asperities absent. Epicranial suture with stem short, endocarina incomplete, not reaching frontoclypeus. Stemmata convex, six in two rows, laterally positioned; ventral row small­ er than dorsal row. Antenna with two ring­like sections, set in membrane. Frontoclypeus triangular, longer than wide. Labrum ( Fig. 8 ) hemispherical, with distal medial emargination; exterior surface with single paired lateral setae on postero­lateral margin, spiny processes absent; interior surface with single paired medial setae and single paired antero­lateral setae. Mandible ( Fig. 9 ) palmate, mesal margin quadri­dentate, with 1 st and 4 th teeth smaller than 2 nd and 3 rd. Maxilla ( Fig. 10 ) with cardo longer than stipes, setae sparse; palpus two­segmented, segment 2 with conical sensory papilla; mala undifferentiated, with apical setae. Labium ( Fig. 10 ) with palpus one­segmented; basal plate fused, ring­like; ligula inconspicuous; hypopharynx densely asperate. Epipharynx densely microtrichate. Thorax. Pronotum pigmented, overlapping head; margin with three paired scoli, three on each side, two antero­laterally positioned and paired, third posterolaterally positioned; mesonotum with two lateral scoli; metanotum with one pair scoli

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on postero­lateral angles. Pronotal anterior scoli and mesonotal anterior scoli shortest. Spiracles annulate, prominent, projecting, without setae on inner margin. Legs ( Fig. 11 ) four­segmented, with sparse long setation; tarsungulus curved. Abdomen. Ten­segmented; segments 1–8 with paired lateral scoli; 1–7 with paired annulate spiracles in dorsal lateral lines. Lateral scoli ( Fig. 12 ) curved, tapered; scoli on segments 1–2 anteriorly directed; scoli on segments 6–7 latero­posteriorly directed; scoli on segments 8 posteriorly directed and not visible in dorsal view. Scoli on segments 6–8 long, almost twice longer than abdominal scoli. Caudal furca ( Fig. 13 ) paired, long, unbranched, tapered, dorsally projected; surface wrinkled, setae present, asperities absent; basal plate narrow, sclerotized. Thoracic and abdominal scoli with surface slightly wrinkled and setose; setation distinct, with basal portion long and bristle portion stiff and barb­like. Shield. Present, welldeveloped up to 7 mm in height, composed of feces and exuviae with four intact head capsules.

Larva ( Fig. 14 ), 4th instar: length 6.3–8.7 mm (n = 10). Body color light yellow, without grey cast. Scoli number and arrangement, setation and trichiation pattern, and fecal shield with characteristics similar to 5 th instar larvae. Shield ( Fig. 15 ): present, well­developed, composed of feces and exuviae with three intact head capsules.

Pupa ( Figs. 16–17 ), length 13.0 mm (n = 1). Color dark brown to black, dorsum with narrow medial cream­colored stripe extending from anterior pronotal margin to 1 st abdominal tergum. Integument smooth, devoid of setation or trichiation. Head hidden by pronotum, not visible in dorsal view. Pronotum with well­developed explanate margin, anterior edge continuous; dorsal surface feebly wrinkled, without dorsal or lateral projections. Abdominal segments 1–3 with margins laterally extended into short lobes; other segments with rounded margins; abdominal segment 9 without urogomphi. Spira­

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cles elevated. Exuvio­fecal shield retained ventrally, compressed and forming point of attachment to stem of vine.

Distribution. Previously published localities for this species include Colombia, Costa Rica and Panama ( Champion 1893; Viana 1968; Borowiec 1999). Viana (1968) provided localities for 17 specimens. One specimen (MCZ) examined for this study was collected in Peru, a new country record.

Material Examined. 111 fourth instar larvae, 42 fifth instar larvae, 5 pupae, and 34 males and females were examined with the following label data:

No data: Bowditch coll. (1, MCZ); Columbia: (2, MCZ); Cartagena , 1871 , coll. Dr. Maak (1, MCZ); Panama: (1, MCZ); Peru: Lima (1, MCZ); Costa Rica: Provincia Puntarenas: Corcovado National Park, Osa Peninsula , 21–30 Nov 1977 , coll. D. H. Janzen (3, INBio); Miramar , 5 May 1977 , coll. F. Vargas (1, UCR); Est. Queb. Bonita, 50 m, Res. Biol. Carara , coll. E. Bello, 17 Mar­ 30 Apr (no year) , coll. P. Campos (1, INBio); Est. Queb. Bonita, 50 m, Res. Biol. Carara , June 1990 , coll. E. Rojas (1, INBio); Est. Quebrada Bonita (Agui­

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rre), R. B. Carara, A. C. Pacifico Central , 100 m, Oct 1994, coll. R. Guzmán (1, INBio) ; Est. Quebrada Bonita, R. B. Carara , 100 m, Feb 1995, coll. R. Guzmán (1, INBio) ; La Palma Coopeunioro, Alfrededores del Albergue Unioro , 250 m, 13–22 Mar 1995, coll. L. Angula (2, INBio) ; Cerro de Oro , ACO­ SA, 270 m, 14–18 Mar 1995, coll. M. Madrigal (1, INBio) ; 300 m, 4–5 Nov 1995, coll A. Azofeifa (2, INBio) ; Est. Agujas, Sendero Zamia , 300 m, 26– 30 Nov 1995, coll. A. Azofeifa (1, INBio) ; Est. Agujas, Sendero Zamia, Rio Agujas , 300 m, 9–8 Mar 1996, coll. A. Azofeifa (1, INBio) ; Est. Rio Bonito, 2.3 Km al O. del Cerro la Gamba , 110 m, 10–30 Apr 1996, coll. E. Fletes (1, INBio) ; San Vito, Las Cruces Biol. Stn. , ex. Mikania sp. , 12 June 2000, coll. C. S. Chaboo (3 pupae; CSCC) ; Provincia Cartago: Parque del Este, Tres Rios , 11 July 1990, coll. J. Segura (1, UCR) ; Provincia San Jose´: Parque del Este , June 1990, coll. J. Seguro (1, UCR) ; Rio Peñas Blancas , north bank, ex. Mi­

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Remarks. The testes varied in size and texture, from soft small rounded structures to large, stiff structures. This variation may be related to maturity of particular individuals. I was unable to determine if the endophallus surface is spiny or not.

The 4 th instar larva is similar to the 5 th instar larva in the number and arrangement of scoli, furcae, setation and trichiation. The only obvious distinctions are in color and size. The shield has a pyramidal shape composed of layers of exuvial skins. Each exuviae was folded over once, with head capsules neatly stacked posteriorly, and the older caudal furcal integument enclosing the more recent one. The head capsules thus provide a convenient count for determining the larval instar.

Figures

Fig. 3. Photograph of aedeagal system of E. columbiana.

Fig. 4. Photograph of spermatheca of E. columbiana (spermathecal muscle removed by KOH).

Fig. 5. Spermathecal system of E. columbiana.

Figs. 6–13. Larva of E. columbiana, 5th instar. 6) Ventral view, scale bar = 1 mm; 7) Dorsal view, scale bar = 1 mm; 8) Labrum, ventral view, scale bar = 0.25 mm; 9) Mandible, dorsal view, scale bar = 0.25 mm; 10) Maxilla and labium, ventral view (half shown), scale bar = 0.25 mm; 11) Proleg, laterosternal view, scale bar = 0.5 mm; 12) First abdominal scoli, dorsal view, scale bar = 0.5mm; 13) Caudal furca, posterior view, scale bar = 0.5 mm.

Figs. 14–15. Larva of E. columbiana. 14) 4th instar; 15) Exuvio­fecal shield.

Figs. 16–17. Pupa of E. columbiana. 16) Dorsal view; 17) Ventral view.