Ophiostoma brevipilosi R.Chang & Z.W.de Beer

Chang, Runlei, Duong, Tuan A., Taerum, Stephen J., Wingfield, Michael J., Zhou, Xudong & Beer, Z. Wilhelm de, 2017, Ophiostomatoid fungi associated with conifer-infesting beetles and their phoretic mites in Yunnan, China, MycoKeys 28, pp. 19-64 : 29-31

Ophiostoma brevipilosi R.Chang & Z.W.de Beer sp. nov. Fig. 8

Etymology.

The epithet brevipilosi refers to the bark beetle vector Tomicus brevipilosus from which all eight isolates of this taxon were obtained.

Description.

Sexual state not observed. Pesotum -like macronematal asexual state predominant. Synnemata simple, dark brown at the base, (179.5-) 227-468 (-667) μm long including conidiogenousapparatus, (22-) 32.5-58 (-69) μm wide at base; cells (13-) 16-26 (-32.5) μm long, conidia hyaline, 1-celled, smooth, oblong, (3-) 3-4.5 (-5.5) × (1.5-) 1.5-2.5 (-3) μm. Hyalorhinocladiella-like asexual state: conidiophores (14.5-) 33-115 (-145) μm long; cells arising directly from the hyphae, (12-) 15-38 (-47) × (1.1-) 1.5-2 (-2.5) μm; conidia hyaline, smooth, obovoid, (2.5-) 3-5.5 (-8) × (1.5-) 2-2.5 (-3) μm.

Culture characteristics.

Colonies hyaline at the beginning, then becoming white to dark. Mycelium superficial on the 3% OA. Colony margin smooth. Colonies on 2% MEA flat, reaching 67 mm diam in 11 d at 25 °C. No growth observed at 5 and above 30 °C. Optimal temperature for growth 20 and 25 °C.

Type material.

CHINA, Yunnan Province, Puer City, from Tomicus brevipilosus on Pinus kesiya bark, 27 Jun. 2010, S.J.Taerum, herbarium specimen of dried culture, PREM 61537 (holotype), CMW41873 = CBS139660 (ex-holotype culture).

Additional specimens examined.

CHINA, Yunnan Province, Puer City, from Tomicus brevipilosus on Pinus kesiya bark, 27 Jun. 2010, S.J.Taerum, PREM 61538, CMW 41624 = CBS139661; CHINA, Yunnan Province, Puer City, from Tomicus brevipilosus on Pinus kesiya bark, 27 Jun. 2010, S.J.Taerum, CMW41662 = CBS139659.

Host.

Pinus kesiya .

Beetle vector.

Tomicus brevipilosus .

Distribution.

At present known only from Yunnan, China.

Notes.

The synnematous asexual state of O. brevipilosi corresponds with similar structures of O. brunneo-ciliatum as described by Linnakoski et al. (2016). The hyalorhinocladiella state resembles those of O. brunneolum , O. macroclavatum , O. pseudocatenulatum ( Linnakoski et al. 2016) and O. poligraphi ( Yin et al. 2016). However, the morphology of these structures is not sufficient to distinguish between the species in the complex, and DNA sequences of the BT and EF gene regions are recommended for accurate species identification.