Neoascochyta zhejiangensis Y. Wang, Y. Tu, X. Chen, H. Jiang, H. Ren, Q. Lu, C. Wei & W. Lv, 2024

Wang, Yuchun, Tu, Yiyi, Chen, Xueling, Jiang, Hong, Ren, Hengze, Lu, Qinhua, Wei, Chaoling & Lv, Wuyun, 2024, Didymellaceae species associated with tea plant (Camellia sinensis) in China, MycoKeys 105, pp. 217-251 : 217-251

Neoascochyta zhejiangensis Y. Wang, Y. Tu, X. Chen, H. Jiang, H. Ren, Q. Lu, C. Wei & W. Lv sp. nov.

Fig. 10

Etymology.

Named after the location where it was collected, Zhejiang Province.

Description.

Sexual morph: undetermined. Asexual morph: Pycnidia smooth, subglobose to ellipsoidal, hyaline. Conidia biconical to subcylindrical, hyaline, smooth- and thin-walled, abundant, generated from pycnidia, aseptate, 4.8–6.5 × 2.9–4.2 µm (av. = 5.6 ± 0.5 × 3.6 ± 0.3 µm, n = 30). Mycelia lateral branching or uniaxial branching, septate, hyaline.

Culture characteristics.

Colonies on PDA reaching 65–69 mm diam. after 7 days at 28 ° C in the dark, margin regular, aerial mycelium flat, whitish; reverse black, white edges. Pycnidia and conidia produced on the colony surface after being cultured for 14 days at 28 ° C in the dark. Colonies on OA reaching 53–57 mm diam. after 7 days at 28 ° C in the dark, margin regular, aerial mycelium flat, whitish; reverse olivaceous, white edges.

Materials examined.

China, Zhejiang Province, Hangzhou City, from diseased leaves of C. sinensis cv. Longjing 43, Jun 2014, Y. C. Wang, Holotype HMAS 352392 , culture ex-type CGMCC 3.24250 = YCW 1107 . Yunnan Province, from diseased leaves of C. sinensis , 23 Mar 2020, Y. C. Wang, culture CGMCC 3 . YCW 1361 .

Notes.

Neoascochyta zhejiangensis is closely related to N. cylindrispora with high statistical support (82 % / 77, ML / PP, Fig. 4 ). Neoascochyta Cylindrispora differs in 1 bp in ITS, 16 bp in TUB 2 and 95 bp in LSU from N. zhejiangensis . In the present study, two strains were isolated from healthy tea plant leaves.

Figures

Figure 4. Phylogenetic tree generated by Maximum Likelihood analysis, based on the combined ITS, LSU, RPB 2 and TUB 2 dataset of Neoascochyta, Paraboeremia, Remotididymella and Stagonosporopsis species. Bootstrap support values above 50 % and Bayesian posterior values above 0.75 are shown at each node (ML / PP). Pleiochaeta setosa CBS 118.25 / CBS 496.63 is used as the outgroup. Ex-type strains are emphasised in bold.

Figure 10. Neoascochyta zhejiangensis YCW 1107 A, B colony on PDA (front and reverse) C, D colony on OA (front and reverse) E mycelia F pycnidia forming on PDA G conidia. Scale bars: 10 μm (E – G).