Meidiama uruguayensis, Schockaert & Curini-Galletti & Ridder & Volonterio & Artois, 2009

Schockaert, Ernest R., Curini-Galletti, Marco, Ridder, Wouter De, Volonterio, Odile & Artois, Tom, 2009, A new family of lithophoran Proseriata (Platyhelminthes), with the description of seven new species from the Indo-Pacific and South America, and the proposal of three new genera, Zoological Journal of the Linnean Society 155 (4), pp. 759-773 : 760-763

MEIDIAMA URUGUAYENSIS SP. NOV.

MEIDIAMA SCHOCKAERTI MARTENS & CURINI- GALLETTI, 1993

Diagnoses: Meidiama lutheri : species of Meidiama with a curved stylet of about 60-Mm long, accompanied by 10–12 needles of 30-Mm long, with a small terminal hook of about one-third to one-quarter of the length of the needles; close to 100 testes.

Meidiama uruguayensis : species of Meidiama with a curved stylet of 80–90-Mm long, accompanied by 19–21 needles of 30–45-Mm long, with a small terminal hook of about one-quarter to one-fifth of the length of the needles; about 50 testes.

Meidiama schockaerti : species of Meidiama with a straight stylet of about 65-Mm long, accompanied by 18–20 needles of 60 Mm, without hook; 9–10 testes in a row.

Occurrence: Meidiama lutheri: Beach of Guarujà , near Santos, Brazil; coarse shell gravel (type locality).

Meidiama uruguayensis: Río de la Plata, Balneario Las Flores, Departamento de Maldonado (34°48′50″S, 55°20′03″W), Uruguay (type locality); Playa Grande , Uruguay; coarse sand between rocks (18/7/2004).

Meidiama schockaerti: Pingüinera beach (Seno Otway), Chili (Magellan Province); coarse sand and gravel (not Punta Arenas, Argentina, as mentioned in the original description of Martens & Curini-Galletti 1993).

Material studied: Meidiama lutheri : two whole mounts, one indicated as lectotype ( SMNH 7348 ); two slides with dispersed sections of several individuals, and of very poor quality, containing very fragmentary information (paralectotypes: SMNH 7349–7350 ) .

Meidiama schockaerti : holotype ( SMNH 7351 ; whole mount) .

Meidiama uruguayensis : four whole mounts and ten sectioned individuals (13 slides) from the type locality; one of the whole mounts is designated as the holotype ( SMNH 7352 ); paratypes UH 371–380 .

Etymology: Meidiama uruguayensis has been named after the country where it was found.

Description: The animals are very long and slender: adults of M. lutheri are around 4-mm long; adults of M. schockaerti are 2–3-mm long and adults of M. uruguyaensis are around 6-mm long (all measured on the whole mounts), with some anterior sensory bristles. Adhesive papillae were not seen in the whole mounts, nor in the sections, but are present in the caudal region of M. lutheri , according to Marcus (1946). The brain is encapsulated, and there is a clear extension of the gut over the brain. The pharynx is directed ventrally, is slightly lobate, and is roughly in the middle of the body ( Fig. 1 ). The epidermis is 1.5–2-Mm thick in M. lutheri , and the cilia are 3-Mm long dorsally and 4-Mm long ventrally; in M. uruguayensis the epidermis is 1–1.5-Mm thick, and the cilia are 2 and 3-Mm long, respectively.

The ovaries are just in front of the pharynx, and the vitellarian follicles are all behind the pharynx, extending over around two-thirds of the postpharyngeal body part. Behind the last, most posterior follicle, the two ovovitelloducts join to form the common female duct. Slightly more posteriorly, this duct enlarges to form the copulatory bursa, in which a short vagina opens ( Figs 1 , 2 ). The female duct continues over a considerable distance (about 0.25 mm) to reach the female pore. From here departs a short genito-intestinal duct, which opens into a large resorbing bursa in the intestine. A connection with the gut is not seen, although some clusters of sperm are observed in the gut at the level of, and even anterior to, the copulatory organ. Very large cement glands run to the female pore, with the gland bodies lying mainly in the most caudal body part. The whole female duct is lined by a rather dense and nucleated epithelium, and is surrounded by spiral muscles. The epithelium is slightly higher in the copulatory bursa and in the most anterior part of the female duct. The muscle layer around the copulatory bursa is also slightly stronger than on the rest of the duct. The epithelium of the ovovitelloducts is very thin and sinuous, and no evidence of a resorbing function was seen.

There are about 50 testes in M. uruguayensis (close to 100 in M. lutheri , according to Marcus 1946: fig. 120), roughly arranged in two rows, and with the first testis found at one-third of the prepharyngeal region, and with the last testes just in front of the ovaries. In M. schockaerti there are only 9–10 testes in a row. The copulatory organ, just in front of the female pore, is directed forwards. It consists of a pair of spherical seminal vesicles, from which long narrow ducts depart ( Fig. 3 ). These ducts join to form a single seminal duct, which enters the prostate vesicle at its proximal pole. The seminal vesicles and the ducts are all surrounded by a strong spiral muscle layer. The prostate vesicle tapers anteriorly, where the stylet is attached, and is recurved over 180°, with both proximal and distal poles directed forwards. The seminal duct can clearly be seen in the centre of the vesicle, with its epithelium forming the prostate glands, the nucleated gland bodies of which are outside and inside the vesicle. In the large male atrium, the curved stylet and the atrial needles are found. The needles are situated in the distal half of the atrium, and are arranged in a half-open girdle, closed at the dorsal side (above the stylet), and open at the ventral side. The prostate vesicle is surrounded by very strong, spirally arranged muscles, which continue over the male atrium, and seem to be attached to the needles. A moderately strong sphincter surrounds the male pore.

In both individuals of M. lutheri , the curved stylet is about 60-Mm long, the needles are 28–30-Mm long, and are also slightly curved. The funnel-like proximal end of the stylet has an opening of 7–8 Mm in diameter, with a strongly thickened wall, whereas the stylet diameter tapers from 5 Mm proximally to 2 Mm distally. The curvature of the very distal end is stronger than in the middle of the stylet, making a precise measurement difficult, as the entire length of the stylets in the whole mounts cannot be seen in the same plane. The terminal opening is oblique and tapering proximally. We counted nine needles, whereas Martens & Curini-Galletti (1993) counted ten, and Marcus (1946) mentions 12. Needles lying above each other are indeed often difficult to discern. Each needle ends in a slender ‘hook’, about one-third of the total length of the needle in the shortest needles, and about one-quarter of the total length in the longest needles.

In M. uruguayensis the stylet is also curved, and is about 90-Mm long in the holotype. At the distal opening it bears a variably ornamented ‘wing’ ( Fig. 4 , inset). The obliquely-placed proximal opening, also with a strongly thickened wall, has a diameter of 20 Mm, and the stylet has a diameter of slightly less than 5 Mm distally. There are about 18 needles, which are 31–37-Mm long, with the shortest needle located in the middle of the circle. The terminal ‘hooks’ have a strongly curved tip, and are a little less than between one-fifth and one-quarter of the total length of the needles. The length of the stylet varies from 76 to 90 Mm (n = 7, mean = 84 Mm); the shortest needles are 26–46-Mm long (n = 6, mean = 32 Mm), and the longest needles are 35–69-Mm long (n = 6, mean = 44 Mm). The individuals with the longest stylet also have the largest needles.

The copulatory organ of M. schockaerti deviates in a number of aspects from that of the other two species. The stylet is straight, close to 65-Mm long, and gradually narrows from 10-Mm wide at the proximal opening (without obvious thickening) to about 3-Mm wide distally. The needles are about 60-Mm long, and are without a terminal ‘hook’. We counted about 18 needles, whereas Martens & Curini-Galletti (1993) depicted 21 needles in a regular arrangement around the stylet. We have the strong impression that the needles are concentrated at one side of the stylet, as in the other species of Meidiama .

Figures

Figure 1. General appearance of a species of Meidiama combined from whole mounts of Meidiama lutheri, Meidiama uruguayensis, and fig. 120 of Marcus (1946). Refer to the Appendix for a list of abbreviations.

Figure 2. Sagittal reconstruction of the female system, seen from the left, of the species of Meidiama, mainly based on sections of Meidiama uruguayensis sp. nov. The lower figure is the continuation of the upper part. Refer to the Appendix for a list of abbreviations.

Figure 3. Reconstruction of the copulatory organ in Meidiama lutheri and Meidiama uruguayensis sp. nov., seen from the left, based on sections of M. uruguayensis sp. nov. and the whole mounts of both species. Refer to the Appendix for a list of abbreviations.

Figure 4. Hard parts of the copulatory organ of Meidiama uruguayensis sp. nov. (A; inset, terminal opening in the holotype), Meidiama lutheri (B), and Meidiama schockaerti (C).