Bradyrhizobium acaciae, Avontuur & Palmer & Beukes & Chan & Tasiya & Zyl & Coetzee & Stepkowski & Venter & Steenkamp, 2022

Avontuur, Juanita R., Palmer, Marike, Beukes, Chrizelle W., Chan, Wai Y., Tasiya, Taponeswa, Zyl, Elritha van, Coetzee, Martin P. A., Stepkowski, Tomasz, Venter, Stephanus N. & Steenkamp, Emma T., 2022, Bradyrhizobium altum sp. nov., Bradyrhizobium oropedii sp. nov. and Bradyrhizobium acaciae sp. nov. from South Africa show locally restricted and pantropical nodA phylogeographic patterns, Molecular Phylogenetics and Evolution (107338) 167, pp. 1-13 : 10

4.1. Description of Bradyrhizobium acaciae sp. nov.

Bradyrhizobium acaciae (a.ca’ ci.ae. N.L. fem. gen. n. acaciae , referring to the host plant genus Acacia , from which the species was isolated).

Cells are Gram-negative, motile, rod-shaped and approximately 2.35 × 0.65 μm in size. Colonies whengrownon YMA at 28 ◦ C after 7 daysare convex and can either be translucent or opaque, cream in colour and less than 1 mm in diameter. Isolates within this species can grow in temperatures ranging from 10 to 40 ◦ C and at a pH between 5 and 10 and cannot grow in the presence of more than 1.5% NaCl (w/v). They are positive for oxidase, urease, β- glucosidase and β- galactosidase activity. Positive reactions were recorded for the following carbon utilization: Larabinose, D-mannose, potassium gluconate, Tween 40, L-asparagine, Tween 80, L-frucotose, D-galactose, D-galacturonic acid, α- D-glucose, Larabinose, L-rhamnose, formic acid, D-saccharic acid, glucuronamide, D- and L- alanine, and L-aspartic acid. Carbon utilization reactions was negative for L-tryptophan, glucose fermentation, hydrolysis of gelatin, D-mannitol, D-maltose, i-erythritol, gentibiose, m-inositol, D-melibiose, L-ornithine, capric acid, adipic acid, citrate utilization, phenylacetic acid, malic acid, α- cyclodextrin, glycogen, N-acetyl-D-galactosamine, glucosamine, D-raffinose, adonitol, D-arabitol, D-cellobiose, D-glucoside, D-psicose, sucrose, D-trehalose, xylitol, D-sorbitol, D-glucoronic acid, glucose-1-phosphate and glucose-6-phosphate. Resistance to ampicillin (10 μg/ml), penicillin (10 μg/ml), erythromycin (30 μg/ml), streptomycin (25 μg/ml), tetracycline (30 μg/ml), neomycin (10 μg/ml), chloramphenicol (30 μg/ml), kanamycin (30 μg/ml) and susceptible to gentamycin (10 μg/ml) and streptomycin (10 μg/ml).

The type strain, 10 BBT (=SARCC 730 = LMG 31409), was isolated from nodules of Acacia dealbata growing in the Gauteng Province of South Africa and can form effective nodules on cowpea ( Vigna unguiculata ) and siratro ( Macroptilium atropurpureum ). The DNA G + Ccontent of the type strain is 63.6 mol%. GenBank/EMBL/DDBJ accession numbers for the genome sequence and gene sequences of this type strain are: whole genome sequence (JACMYK000000000), 16S rRNA ( LR877293 ), atpD ( LR877274 ), glnII ( LR877284 ), dnaK ( LR877288 ), gyrB ( LR877279 ), rpoB ( LR877266 ) and nodA ( LR880919 ) .