Buchholzia

Lu, Yajing, Xie, Zhicai & Zhang, Junqian, 2018, Preliminary taxonomical investigation of soil enchytraeids (Clitellata, Enchytraeidae) from south region of Tibet, China, Zootaxa 4496 (1), pp. 395-410 : 407

Buchholzia sp.

( FigURES 7A–G )

Examined material. XZO201510004, XZO201510008, XZO201510009, XZO201510010, FOUR immaTURE SPEcimEN, STaiNEd, WhOlE-mOUNTEd. MORE ThaN 20 SPEcimENS iN 70% EThaNOl, all immaTURE.

Descriptions. MEdiUm SiZE. BOdY cOlOR WhiTE iN ViVO. BOdY lENgTh 3.6–4 mm. BOdY WidTh 140–175 Μm. SEgmENTS 32–36. A maXimUm OF 5 chaETaE PER bUNdlE. ChaETaE WEaklY SigmOid, WiThOUT NOdUlUS ( Fig. 7A ). ChaETaE 2–3 PER bUNdlE aT aNTERiOR SEgmENTS, 4–5 PER bUNdlE aT POSTERiOR SEgmENTS. EPidERmal glaNd cEllS PRESENT, iRREgUlaR aNd cONSPicUOUS, 2–4 TRaNSVERSE ROWS PER SEgmENT ( Fig. 7F ). BRaiN SlighTlY cONcaVE POSTERiORlY. ThREE PaiRS OF PhaRYNgEal glaNdS PRESENT iN IV–VI, all WiTh dORSal cONNEcTiON. PaiRS OF V aNd VI laRgER ThaN ThaT OF VII, WiTh WidE cONNEcTiON ( Fig. 7C ). ONE PaiR OF cONSPicUOUS OESOPhagEal aPPENdagES cONFiNEd TO IV, WiThOUT bRaNchES ( Fig. 7D ). INTESTiNal diVERTicUla aT VII/VIII, OccaSiONallY iN VI/VII ( Fig. 7B ). ANTESEPTalE WiTh FUNNEl aNd Small PaRTS OF NEPhRidial bOdY ( Fig. 7G ). TWO TYPES OF cOElOmOcYTES ( Fig. 7E ). MUcOcYTES laRgER aNd lESS NUmEROUS ThaN lENTicYTES. COElOmO-lENTicYTES abUNdaNT, SiZE aS 1/4–1/3 aS mUcOcYTES. DORSal blOOd VESSEl ORigiNaTiNg FROm ThE ENd OF VII. BlOOd cOlORlESS.

Differential diagnosis. OUR SPEcimENS cORRESPONd WEll TO Buchholzia appendiculata (BUchhOlZ, 1862) ( SchmElZ & COlladO 2010), iN haViNg UP TO 5 chaETaE PER bUNdlE, laRgER SiZE OF cOElOmO-lENTicYTES aNd ThE ShaPE OF ThE iNTESTiNal diVERTicUlUm. B. appendiculata REPROdUcES SEXUallY aNd aSEXUallY bY FRagmENTaTiON. AdUlT SPEcimENS OR SigNS OF aSEXUal REPROdUcTiON (FRagmENTS) WERE NOT FOUNd, ThEREFORE ThE SPEciES idENTiTY OF OUR SPEcimEN REmaiNS UNcERTaiN. ThiS gENUS iS NEW REcORd FOR ChiNa.

Figures

FIGURE 7. Micrographs of Buchholzia sp. in vivo. A. Chaetae in IV. B. Intestine diverticulium at VI/VII. C. Pharyngeal glands. D. Oesophageal appendages in IV (black arrows). E. Coelomo-mucocytes (black arrow), lenticytes (white arrow). F. Epidermal gland cells in anterior segment (black arrow). G. Nephridium in VII/VIII. Scale bars: A,E, 10 µm; D,F,G, 25 µm; B,C, 50 µm.