Pseudogeomyces lindneri Zhi. Y. Zhang & Y. F. Han, 2023

Zhang, Zhi-Yuan, Han, Yan-Feng, Chen, Wan-Hao & Tao, Gang, 2023, Additions to Thelebolales (Leotiomycetes, Ascomycota): Pseudogeomyces lindneri gen. et sp. nov. and Pseudogymnoascus campensis sp. nov., MycoKeys 95, pp. 47-60 : 47

Pseudogeomyces lindneri Zhi. Y. Zhang & Y. F. Han sp. nov.

Fig. 3

Etymology.

Named after Daniel Lindner, for acknowledging his contributions to the modern taxonomy of Pseudogymnoascus and its related taxa.

Type.

Kaili City , Guizhou Province, China 27°16'98"N, 108°81'46"E, isolated from the green belt soil, July 2022, Zhi-Yuan Zhang (holotype ZY H-22.003, ex-type ZY 22.003, ibid., ZY 22.004) .

Geographical distribution.

Guizhou Province, China.

Description.

Culture characteristics (14 days at 25 °C): Colonies on PDA 15-16 mm in diameter, white to pale pink, raised, fluffy, irregular, producing abundant caesious exudates; reverse: brown to cinnamon. Colonies on MEA 18-19 mm in diameter, off-white, felty, with radial grooves, nearly round, exudates and diffusible pigments absent; reverse: brown to cinnamon. Colonies on OA 25-26 mm in diameter, white, aerial mycelia sparse, flat, nearly round, exudates and diffusible pigments absent; reverse: white. Colonies on CMA 34-35 mm in diameter, white, aerial mycelia sparse, flat, nearly round, margin regular, exudates and diffusible pigments absent; reverse: white.

Hyphae hyaline, smooth, branched, septate, 1.0-2.0 μm in diameter. Conidiophores solitary, rare branches, hyaline, smooth, arising from erect or geniculated hyphae, sometimes reduced to conidiogenous cells, erect, usually bearing two to four conidiogenous cells at the tip. Conidia hyaline, rough, verrucosa, solitary, obovoid, globose to subglobose, 3.0-7.5 × 2.5-5.5 µm (av. 4.8 × 3.8, n = 50), borne on hyphae, short protrusions, side branches or in conidiophores separated by connective cells. Intercalary conidia hyaline, globose to subglobose, fusiform, with both truncate 3.5-6.5 × 3.0-4.5 µm (av. 4.9 × 4.0, n = 50). Chlamydospores not observed. Sexual morph undetermined.

Notes.

Based on multi-locus phylogenetic analyses (Figs 1 , 2 ) and morphological characteristics, Pseudogeomyces lindneri is proposed as the type species of Pseudogeomyces . The isolates ZY 22.003, ZY 22.004 and ZY 22.005 formed a single phylogenetic clade and were separated from the other four unidentified isolates (12NJ08, 17WV09, 23WI14 and 23WI08) under Pseudogeomyces . Morphologically, Pseudoge. lindneri differed from other taxa under the family Thelebolaceae in terms of the presence of two to four irregular branches at the tip of the conidiophores and that the conidia and intercalary conidia are generally connected by connective cells in a chain ( Kuehn 1958; Van Oorschot 1980).

Figures

Figure 1. Phylogram generated from a Maximum Likelihood analysis of sequences of Thelebolales, based on ITS and LSU. ML bootstrap values (≥ 75 %) and Bayesian posterior probability (≥ 0.75) are indicated along branches (BP / ML). The new taxa are highlighted in bold and blue and " T " indicate ex-type cultures.

Figure 2. Phylogram generated from A Maximum Likelihood analysis of sequences of Thelebolaceae, based on ITS, LSU, EF 1 A, RPB 2 and MCM 7. ML bootstrap values (≥ 75 %) and Bayesian posterior probability (≥ 0.75) are indicated along branches (BP / ML). Clades are identified using clade nomenclature (A to O) formally defined by Minnis and Lindner (2013). The new taxa are highlighted in bold and blue and " T " indicate ex-type cultures.

Figure 3. Morphology of Pseudogeomyces lindneri sp. nov. a-d colony on PDA, MEA, OA and CMA after 14 d at 25 ° C (upper surface and lower surface) e-q Conidiophore, Conidia and Intercalary conidia. Scale bars: 10 mm (a-d); 10 μm (e-q).