Philaccolilus ameliae Balke, Larson, Hendrich & Konyorah, 2000

Balke, Michael, Panjaitan, Rawati, Surbakti, Suriani, Shaverdo, Helena, Hendrich, Lars, Van Dam, Matthew H. & Lam, Athena, 2022, NextRAD phylogenomics, sanger sequencing and morphological data to establish three new species of New Guinea stream beetles, Alpine Entomology 6, pp. 51-64 : 51

Philaccolilus ameliae Balke, Larson, Hendrich & Konyorah, 2000

Figs 2E , 4 , 5B , 7A-D , 8C, D

Philaccolilus ameliae Balke, Larson, Hendrich & Konyorah, 2000: 35.

Type locality.

Gusap, Markham Valley, Morobe Province, Papua New Guinea.

New material studied.

(KSP, MZB, NARI, ZSM). 7 exs, PNG, Morobe, Herzog Mts., Patep, 700 m, 20.xi.2006, - 6.9711, 146.6315, Balke & Kinibel ( PNG 105); 14 exs, PNG, Sandaun, Mianmin, 1,000 m, 20.x.2008, -4.8881, 141.5686, Ibalim ( PNG 191); 2 exs, PNG, Sandaun, Mianmin (river), 700 m, 21.x.2008, -4.8809, 141.5284, Ibalim ( PNG 197); 14 exs, PNG, Sandaun, Mianmin area , 800 m, 6.i.2010, -4.9092, 141.6159, Ibalim & Pius ( PNG 239); Papua, Sarmi, Waaf, N Foja Mts , riverbank, 120 m, 23.ix.2014, -2,3445, 138,7395, Balke & Menufandu (Pap030); 9 exs, Papua, Dekai, upper Brazza , 273 m, 2./ 3.vi.2015, -4,7410, 139.6542, Sumoked (Pap044) .

Note.

All of these specimens from central New Guinea were assigned to P. ameliae based on matching them with a short CO1 sequence with four individuals from Morobe: Herzog Mts., Patep, which is part of the type area of P. ameliae . For the 300 basepair fragment obtained, the identity with Sandaun and Waaf specimens matched 99%. The short fragment for the Patep specimens was not used for the haplotype network in Fig. 5 .

Description.

Same as for the species complex. Moderately to larger sized member of the genus: the specimens from the Waaf population are on average (5.1 mm) smaller than from PNG localities (5.5 mm). The lateral spot of subbasal band not or at most hardly so in contact with lateral margin. The extend of the elytral spots is variable (Fig. 2H ), the most extended configuration of the paler spots is only observed in few specimens and sometimes even only on one elytron. The outer spot of the subbasal band does not reach the elytral margin, or at most, in few specimens, vaguely so (as in Fig. 3D, E ) (Table 1 ).

The medio-discal spot is typically narrow and longish, sometimes connected to the postmedial band.

Size. Paratypes from PNG: Wau TL (N=15) 5.3-5.7 mm (av. 5.5 mm), TW 2.9-3.1 mm; specimens from PNG: Mianmin (N=15) 5.3-5.9 mm (av. 5.5 mm), TW 2.9-3.1 mm; specimens from Papua: Waaf (N=9) 4.9-5.3 mm (av. 5.1 mm), TW 2.9-3.0 mm.

Genitalia. Median lobe of aedeagus as in Fig. 7A-D .

Distribution.

Widespread from central to east New Guinea (Fig. 4 ).

Habitat.

Collected from different stream types, but usually more sun exposed.

Figures

Figure 2. A-G. Dorsal habitus of Philaccolilus species in the P. ameliae complex, as well as P. kirana and P. intania, appendages mostly removed; brightness of some paler spots digitally slightly enhanced for clarity, without altering the basic color tone; H. schematic drawings of color pattern variation in P. ameliae species complex, with explanation of elytral pattern positions (modified from Balke et al. 2000).

Figure 3. Lateral habitus of Philaccolilus species; brightness of some paler spots digitally slightly enhanced for clarity, without altering the basic color tone. A. P. kirana; B. P. intania; C. P. weylandensis (Weyland Mts., basal lateral spot clearly reaching elytral margin); D. P. weylandensis (Digul River, basal lateral spot vaguely reaching elytral margin); E. P. ameliae (Waaf, basal lateral spot clearly isolated from elytral margin).

Figure 4. Distribution of Philaccolilus species in New Guinea. Dots - P. ameliae complex, stars - P. kirana and P. intania here described from the Bird's Head Peninsula.

Figure 5. Results of the genomic nextRAD as well as mitochondrial DNA CO 1 data sequencing of Philaccolilus species in New Guinea. A. P. febrina in the Bird's Head Pensinsula; B. P. ameliae and P. weylandensis in New Guinea main body; C. P. kirana and P. intania from the Bird's Head Peninsula (localities see A). Results of the structuring analysis of nextRAD data depicted as barplots, the CO 1 data as haplotype network, subs. = substitutions.

Figure 7. Median lobe of aedeagus of Philaccolilus species (left, lateral, right, ventral view) A, B. P. ameliae (from Brazza River); C, D. P. ameliae (paratype from Wau); E, F. P. febrina (from Tamrau Mts.); G, H. P. weylandensis (from Weyland Mts.); I, J. P. weylandensis (from Digul River).

Figure 8. Last male and female ventrite of Philaccolilus A, B. As in P. kirana and P. intania; C, D. As in P. ameliae, P. febrina and P. weylandensis. Not to scale. In C. with last and penultimate ventrites to illustrate the tuft of long golden setae on penultimate ventrite; E. P. febrina male in ventral view, not to scale.