Onchoproteocephalidea

Marmolejo-Guzman, Linda Yacsiri G., Iv, David, Hern, an G., andez-Mena, Castellanos-Martínez, Sheila & Aguirre-Macedo, M. Leopoldina, 2022, Linking phenotypic to genotypic metacestodes from Octopus maya of the Yucatan Peninsula, International Journal for Parasitology: Parasites and Wildlife 19, pp. 44-55 : 49-50

3.3.2. Onchoproteocephalidea

A single sequence of the 28S rDNA gene was amplified for Phoreiobothrium sp. (360 bp in length) and Acanthobothrium sp. (1060 bp in length). Both sequences were aligned with 60 sequences of Onchoproteocephalidea providing an aligned dataset of 1155 bp with nucleotide frequencies of A = 0.217083, C = 0.224792, G = 0.325049, T =

49

0.233076, and a ML value of ln = - 9067.566753 ( Fig. 6 ). The cestodes phenotypically identified as Acanthobothrium and Phoreiobothrium grouped into non-monophyletic clades with their respective congeners ( Fig. 6 ). Acanthobothrium sp. ex O. maya formed a highly supported group (Bt = 83) with Onchobothrium , Uncibilocularis , and Potamotrygonocestus ( Fig. 6 ). In addition, Acanthobothrium sp. ex O. maya nested with specimens of Acanthobothrium sp. ex Diplectrum formosum ( Perciformes ) (Bt = 100) and Octopus vulgaris (Octopodiformes) (Bt = 100). No genetic variation was found between Acanthobothrium sp. ex O. maya and ex D. formosum , whereas the genetic distance between Acanthobothrium sp. ex O. maya and ex O. vulgaris varied between 2.7% and 2.9%; while it ranged from 4.1% to 12.2% for the remaining species ( Fig. 6 ).

The plerocercoids Phoreiobothrium sp. ex O. maya formed a clade with the genera Phyllobothrium , Platybothrium, Prosobothrium , and

Triloculatum (Bt = 92). In addition, plerocercoids of Phoreiobothrium sp. ex O. maya nested with those found in Opsanus beta ( Batrachoidiformes ) without intraspecific genetic variability. The interspecific genetic distance with other species of Phoreiobothrium ranged from 5.1% to 15.5%.

Figures

Fig. 6. Phylogenetic tree based on the Maximum Likelihood analysis of the species of the Order Onchoproteocephalidea found in Octopus maya constructed on partial large subunit ribosomal gene (28S) (likelihood = – 9067.566753). Bootstrap support values for ML are provided at the nodes; ex = host; the stage of development of the cestode in parentheses.