Trochila xishuangbanna (W.Y. Zhuang) Quijada, 2021

Gomez-Zapata, Paula Andrea, Haelewaters, Danny, Quijada, Luis, Pfister, Donald H. & Aime, M. Catherine, 2021, Notes on Trochila (Ascomycota, Leotiomycetes), with new species and combinations, MycoKeys 78, pp. 21-47 : 21

Trochila xishuangbanna (W.Y. Zhuang) Quijada comb. nov.

Calycellinopsis xishuangbanna ≡ Calycellinopsis xishuangbanna W.Y. Zhuang, Mycotaxon 38: 121 (1990). [Basionym]

Notes.

The genus Calycellinopsis was proposed with a single species, C. xishuangbanna , which is a petiole-inhabiting fungus ( Zhuang 1990). The genus was placed in Dermateaceae because of its isodiametric dark brownish excipular cells ( Zhuang 1990). In 2002, a second collection of the same species was sampled (HMAS:187063), which was sequenced ( Zhuang et al. 2010). Additional morphological details were provided, and the genus was placed in Helotiaceae ( Zhuang et al. 2010). Trochila was treated in Dermateaceae until recently because of its excipular features ( Fuckel 1869; Karsten 1869; Saccardo 1884; Lambotte 1888; Lumbsch and Huhndorf 2010). Collections of Calycellinopsis have a well-developed excipulum, with an outer layer of angular to isodiametric cells with brownish walls and cortical cells at flanks and margin with protruding hyaline cells. The medullary excipulum is subhyaline and composed of textura angularis to textura intricata ( Zhuang 1990; Zhuang et al. 2010).

Species in Trochila usually have a poorly developed excipulum. For example, T. bostonensis and T. craterium produce only a thin layer of globose to angular dark excipular cells (von Höhnel 1917; Greenhalgh and Morgan-Jones 1964; Baral and Marson 2005). However, other species, such as T. laurocerasi and T. urediniophila , have a well-developed excipulum (op. cit.). The excipulum of Calycellinopsis is very similar to those species of Trochila with a well-developed excipulum, composed of an outer layer of dark textura globulosa - angularis and an inner layer of hyaline medulla made of textura angularis - porrecta - intricata. At the flanks and margin of the excipulum, Calycellinopsis has protruding hyaline cells similar to Trochila species with a well-developed excipulum (Fig. 4 ). Although limited details about the living features can be obtained from the original description of Calycellinopsis , its hymenial features are consistent with Trochila . The ascospores of Calycellinopsis are described with several guttules, a feature that is also observed in species of Trochila .

Figures

Figure 4. Morphological features of Trochila urediniophila, holotype collection (PUL F 27668) a 1 - a 4 dried apothecia growing on uredinia of Cerotelium fici a 2, a 3 substrate (uredinia) on which the apothecia grow (arrows) b 1 transverse section of apothecia; arrow pointing out the substrate b 2, b 3 details of excipulum at margin and upper flanks b 4 cells at base c 1 - c 3 asci d 1 paraphyses e 1 - e 3 ascospores e 2, e 3 oil drops (LBs) inside ascospores. Mounted in: Congo Red (c 1, e 2), H 2 O (b 2, c 3, d 1, e 1, e 3), KOH (b 1, b 3, b 4, c 2). Scale bars: 1 mm (a 1 - a 3); 500 µm (a 4); 200 µm (b 1); 50 µm (b 2); 20 µm (b 3, b 4, c 2, c 3, d 1); 2 µm (c 1, e 1 - e 3).