Campylaimus bonariensis, Villares & Martelli & Russo & Pastor, 2013

Villares, Gabriela, Martelli, Antonela, Russo, Virginia Lo & Pastor, Catalina, 2013, Three new species and one new record of Campylaimus (Diplopeltidae, Nematoda) from Argentine coasts (Buenos Aires and Santa Cruz, Argentina), Zootaxa 3613 (1), pp. 83-96 : 85-88

Campylaimus bonariensis sp. nov.

( Fig. 2 (A–I), Fig. 5 (E–F); Table 1)

Measurements: see Table 1.

Type material: Male holotype, registration number MACN-In 38917, female allotype, registration number MACN-In 38918, one male paratype and two females paratype, registration number CNP NEM 643, 644, 645.

Type locality. Arroyo Pareja , Buenos Aires province, Argentina. Coordinates: 38°54’S, 62°04’W; water depth: low littoral. Collected by Antonela Martelli, 20 March 2011 .

Etymology. From the Spanish words “ Buenos Aires ”, in reference to the province where it was found.

Description. Male (holotype): Body length medium-sized. Cuticle moderately striated, approximately 2 µm thick. Head narrow, tapering towards the anterior end of the body, with a clearly visible cap. Oral opening located subterminally, on dorsal side of body. Lips absent. Four cephalic setae (5 µm long) located posterior to the level of the oral opening. Somatic setae absent. Amphidial fovea consisting of two parallel arms, situated posterior to the cephalic setae, the long ventral arm expanding into broad lateral alae extending the length of the body to the tail tip and the shorter amphidial branch measuring 60 µm long, with both branches measuring 5 µm wide or 2.4 times the cephalic diameter. The pharynx measures 145 µm long.

Reproductive system diorchic, testes opposed. Spicules equal in length, 32 µm long measured along the arch, slender and arcuate, proximal end with well-developed cephalization. Gubernaculum tubular, 11µm long. Three precloacal papillae are present.

Tail 150 µm long (c' = 7.5), cylindrical, gradually tapering toward the distal end with swollen tip. The tip of the tail has two setae, 3 µm long.

Female (allotype): similar to male in general body shape. Gonads paired, opposed; ovaries outstretched, not reflexed. Anterior and posterior ovaries located on the left and right side of intestine, respectively. Vulval opening ventral, located in the middle of the body. Vagina short, not thickened. Two glands surrounding the vulva. Tail 150 µm long (c' =7.5).

Discussion. Campylaimus bonariensis sp. nov. is characterized by the shape of the spicule and gubernaculum and by the presence of three precloacal papillae.

C. bonariensis sp. nov. resembles: C. abnormis Thanh & Gagarin, 2011 in the value of a, b and c ratios and the dorsal oral opening but differs in the length of the body, the length of the spicule, the length of the gubernaculum and the presence of precloacal papillae; C. tkatchevi Tchesunov, 1974 in the value of b and c, the dorsal oral opening but differs in the length of the body, the length of the spicule, the value of a and the presence of precloacal papillae; C. arcuatus sp. nov. in the shape of the body, the value of a, b and c, the shape of the tail and the length of the gubernaculum but differs in the length and shape of the spicule, the shape of the gubernaculum and the number of precloacal papillae; C. patagonicus sp. nov. in the shape of the body, the value of c, the length of the spicule and gubernaculum but differs in the shape of the head and tail, the shape of the spicule and gubernaculum, the value of a, b and the number of precloacal papillae (Table 5).

C. bonaeriensis sp. nov. differs from all known species by the presence of precloacal papillae: from C. rimatus Vitiello, 1974 , C. striatus Boucher & Helléouët, 1977 and C. ponticus Sergeeva, 1981 by the presence of gubernaculum; from C. mirus Gerlach, 1950 and C. lefeveri Gerlach, 1956 by the body shorter than 1000 µm and from C. inaequalis Cobb, 1920 and C. gerlachi Timm, 1961 by the dorsal oral opening.

Figures

FIGURE 2. C. bonariensis sp. nov. A. Amphidial fovea and cephalic setae on anterior end of male holotype; B. Head and buccal cavity of male holotype; C. Head and buccal cavity of female allotype; D. Amphidial fovea and cephalic setae on anterior end of female allotype; E. Posterior end of male holotype; F. Copulatory apparatus, spicule, gubernaculum and precloacal papillae of male holotype; G. Spicule and gubernaculum of male holotype; H. Vulva of female allotype; I. Posterior end of female allotype. Scale bar: 20 µm.

FIGURE 5. A) Entire female allotype of C. patagonicus; B) Entire male of holotype of C. patagonicus; C) Entire male holotype of C. arcuatus; D) Entire female allotype of C. arcuatus; E) Entire female allotype of C. bonariensis; F) Entire male holotype of C. bonariensis. Scale bar: 50 µm.