Blepharisma penardi, Yan & Fan & Chen & Li & Warren & Al-Farraj & Song, 2016

Yan, Ying, Fan, Yangbo, Chen, Xiangrui, Li, Lifang, Warren, Alan, Al-Farraj, Saleh A. & Song, Weibo, 2016, Taxonomy and phylogeny of three heterotrich ciliates (Protozoa, Ciliophora), with description of a new Blepharisma species, Zoological Journal of the Linnean Society 177 (2), pp. 320-334 : 324

BLEPHARISMA PENARDI SP. NOV. ( FIGS 1E, F, K, L ,

4A–I , 6A, C, D; TABLES 1, 3)

Synonyms: Blepharisma lateritium sensu Penard, 1922

Figures

Figure 1. Morphology and infraciliature of Anigsteinia clarissima (A–D), Blepharisma penardi sp. nov. (E, F, K, L), and Blepharisma undulans (G–J). A, left view of a typical individual of A. clarissima. B, paroral membrane of A. clarissima, anterior (double arrowheads) and posterior (arrow) parts are formed of dikinetids with only left basal body ciliated, middle part (arrowhead) formed by trikinetid fragments with all basal bodies ciliated. C, D, general infraciliature of A. clarissima, arrows show paroral membrane. E, left view of a typical individual of B. penardi sp. nov. F, L, dorsal (F) and ventral (L) views of infraciliature of B. penardi sp. nov. from the same specimen, arrow marks paroral membrane. G, left view of a typical individual of B. undulans. H, paroral membrane of B. undulans, anterior part with only right basal bodies ciliated and posterior part with both basal bodies ciliated. I, J, right (I) and left (J) views of B. undulans showing the infraciliature, arrow points to paroral membrane. K, paroral membrane of B. penardi sp. nov., anterior part consists of ciliated monokinetids, posterior part consists of dikinetids with only left basal body ciliated. Abbreviations: AZM, adoral zone of membranelles; CV, contractile vacuole; LCV, lacunar contractile vacuole system; SK, somatic kineties. Scale bars = 100 μm (A, C, D), 70 μm (E, G, I, J, L), 50 μm (B, H, K).

Figure 4. Photomicrographs of Blepharisma penardi sp. nov. from life (A–E, I) and after protargol staining (F–H), and Blepharisma undulans from life (J, M) and after protargol staining (K, L). A, C, D, left view, to show the shape variants, arrows in C, D mark the contractile vacuole. Note the cell colour: colourless (A) and dark-brownish (C). B, cytoplasmic granules. E, portion of cortex, showing the tiny, densely arranged cortical granules. F, G, general infraciliature. H, portion of buccal field, arrows point to paroral membrane. I, macronucleus. J, left view of a typical individual. K, right view, to show the two macronuclei. L, posterior portion of oral field, arrows indicate paroral membrane. M, to show cortical granules, note there are some white granules (arrows) amongst the coloured ones. Abbreviations: CV, contractile vacuole; Ma, macronucleus. Scale bars = 70 μm (A, C, D, F, G, J, K), 30 μm (H), 15 μm (B, E, I, L, M).

Figure 6. Nominal isolates of Blepharisma penardi sp. nov. (A, C, D), Blepharisma steini (B), and Blepharisma undulans (E–K). A, C, D, B. penardi sp. nov., originally described as an unnamed variety of Blepharisma lateritium, from Penard (1922). B, B. steini, from Kahl (1932). E, B. undulans, from Bhandary (1962). F, B. undulans, from Lee & Shin (2009). G, B. undulans, from Kahl (1932). H, B. undulans, from Cela (1972). I, B. undulans, from Suzuki (1954). J, K, B. undulans, from Dragesco & Dragesco-Kernéis (1991). Abbreviation: Ma, macronuclear nodules. Scale bars = 90 μm (B, I), 80 μm (G, F) 50 μm (C, E, H), 40 μm (J, K).