Neoperla orthonema, Zwick & Zwick, 2023

Zwick, Peter & Zwick, Andreas, 2023, Revision of the African Neoperla Needham, 1905 (Plecoptera: Perlidae: Perlinae) based on morphological and molecular data, Zootaxa 5316 (1), pp. 1-194 : 116-118

52. Neoperla orthonema n. sp.

( Figs. 294–304 )

Type material studied: Republic of Ghana: J Holotype ( NEOP 171, slide Z17_037_sp_Ax \GH.10), Ghana Volta region, Agumatsa waterfalls, Wli, St. 10 19.11.1993 H. Olsvik light trap; NUFU project ( ZMBN). 3♀ paratypes, Ghana, same data as holotype (slides GH.10\2, GH.11\2, ZMBN). 1♀ Paratype, Ghana Volta Region, Wli Falls, Afegame [7.12°N, 0.59°E], St. 12 07.03.1993 J.S.Amakye & J. Kjaerendsen light trap; NUFU-project ZMBN Sample no. 1 ( NEOP 169, slide GH.09\1, ZMNS); 4♀ paratypes, Ghana Western region, Ankasa game prod. Reserve [5.44N, 2.07W, 47m], 10–16.12.93 St. 12 J. Kjaerendsen & T. Andersen light trap; NUFU-project ZMBN Ref. No. 3 (GH.01/17, NEOP 164; NEOP 168, slides GH.01/6, /9, /16, /17; SMNS); 1♁ paratype ( NEOP 170), 1♀ paratype ( NEOP 165, slide GH12\1), 1♀ (Slide Z17.073) Ghana Volta Region, Wli Falls, Afegame, St. 10 08.03.1993 J.S.Amakye & J. Kjaerendsen light trap; NUFU-project ZMBN Sample no. 12; 1♁ paratype, Ghana Volta Region, Wli Falls, Afegame, St. 12 07.03.1993 J.S.Amakye & J. Kjaerendsen light trap; NUFU-project ZMBN Sample no. 1); 1♁ paratype, Ghana Volta Region, Wli Falls, Afegame, St. 12 07.03.1993 J.S.Amakye & J. Kjaerendsen light trap; NUFU-project ZMBN Sample no. 1 ( NEOP 166, slide 98\9, SMNS). Republic of Cameroon, 4♁ p aratypes: Muyuka [4.7217N, 9.6408E; 305m], Victoria Div., BRIT. CAMEROONS,VI-25-49 B.Malkin at Light (Slides Z17.021-023; ZMB & SMNS); 1♁ paratype, 7 mi S Ebolowa [2.92N, 11.16E], 580m, 15.–17-X-1966 Ross & Leech CASENT8413020 (penis slide). République de Côte d’Ivoire: 1 J paratype, 15 km N Lakota [5.86N, 5.68W] 23.Oct. 1971 white light trap J.A.Gruwell ( USNM 152) ( Figs 294–299 ). Togolese Republic, 1♁ paratype, penis in microvial, 2♀, paratypes (Z16/131, NEOP 167 and Z16/132, NEOP 161; SMNS) Amoutchou, 28.11.1985, V. Landa (Z16.132).

Additional material studied: 3♀, Ghana , same data as holotype, 4♀ slides, Ghana Volta Region , Wli Falls , Afegame, St. 6 11.03.1993 J.S.Amakye & J. Kjaerendsen light trap (slides Afr.Z 98\09, Afr. Z98\14; GH.03/3, Ghana ♀ 14; SMNS) . Ghana Volta Region , Wli Falls , Afegame , St. 10 11.03.1993 J.S.Amakye, J. Kjaerendsen light trap (♀ slides: Ghana ♀ 2, Ghana ♀ 4, Z17/73; Ghana ♀ 10; 1♀ (Slide Z 17.073); 3♁, 4♀, Ghana Western region , Ankasa game prod. Reserve [5.44N, 2.07W, 47m], 10–16.12.93 St. 12 J. Kjaerendsen & T. Andersen light trap; NUFUproject ZMBN Ref. No. 3 (slides GH1\18–20); 1♁ (penis in mv), 2♀ ( NEOP163 ) : Ghana Subri R. [5.22851, - 1.7481], 05.11.1993 light trap; NUFU project, Uni Bergen. 4♁, 1♀, GHANA, Western Reg. , Ankasa Resource Res., Nkwanta Camp 05°16.566’N 002°18342’ 7–8 Jun 2005 K.B.Miller, colr. ( BYU, slide Z18.76) . Republic of Guinea: 1♁, Kousankoro no.2, 21.10.84 ( SMNS, gift J.M.Élouard; slide 16/95) . 4♁, Guinea , piège lumineuse, sans étiquette (slides Z18.66 & 67, gift J. M. Élouard) . Republic of Liberia: 2♁, 2♀, Suakoko [7.11°N, 9.59°W 349m] 31 Jan 52 Blickenstaff (slides USNM 8 , 11 , 12 ) ; 1♁, same, except 3–9 May 1952 (slide USNM 41 ) . Republic of Sierra Leone: 1♁, Njala Sierra Leone Afr. XI-16–XII-4 35 \ Van Zwaluvenburg & McGough ( USNM 13 ) . Togolese Republic: 1♀, Amoutchou , 28.11.1985, V. Landa ( SMNS, Z16.132 ) .

Habitus. WL of males 8.2–11.0mm, of females 11.7–13.0mm. Uniformly yellowish ochre, the brown ocellar mark is small. Tip of flagellum faintly infuscate, cercus yellow.

Male ( Figs. 294–300 ). Sternites unmodified. A triangular sclerite on T7 has a narrow caudal process with single SB at tip and a smooth keel along rear face ( Fig. 294 ); some SB in the intersegmental fold where the sclerite is articulated with T8. Sclerite on T8 T-shaped, with a distinct hump with numerous SB in front ( Fig. 295 ). T9 with a median depression between pilose lateral swellings with many SB. HT10 short, regularly curved in dorsal and lateral views, sharply pointed, mediobasal callus tongue-shaped.

Penis ( Figs. 296–299 ) a straight slender tube, distal third membranous except for long lateral sclerites between paired dorsal spine patches and a pair of more distal ventral spine patches ( Fig. 300 ). Tips of spines point in opposite directions, spines in the ventrodistal groups point towards the penis base. Base of endophallus dorsomedially ( Fig. 296 : eb) with patch of erect slender sharp spines. There are scattered slender spines on the wide endophallus base. Distally, the endophallus is a narrow tube with three spine rows: two regular rows of conical spines dorsally plus a single regular row of more slender and locally also longer spines ventrally ( Fig. 296 , inset). Differences between rows diminish distally, all continue to the tip of the endophallus.

Female. As for the group ( Fig. 301 ).

Egg ( Figs. 302–304 ). Size 345–380µm long, on average 1.85 times longer than wide. Numerous (approximately 30) straight striae, each consisting of a wide rib associated with a slightly narrower rib ( Fig. 303 ). The ribs are separated by a single row of micropunctures. All ribs are impunctate, micropyles are located on top of the narrow ribs (similar to Fig. 312 , after Moore 1991). No collar, the wide ribs end in oval depressions on a thickened ring around the anchor pole, the narrow ribs end between them, slightly more distant from the edge ( Fig. 304 ). Anchor cavity shallow. Operculum bluntly rounded, with faint reticulation, low impunctate strips separate finely punctate polygones.

DNA ( Figs. 491–492 , 496 ). A total of 10 specimens from Togo and Ghana were sequenced for the COX1 DNA barcode fragment and with the genome-skimming approach, providing limited representation of the geographical distribution of the species. This also includes the male holotype from Ghana for the COX1 DNA barcode fragment. The monophyly of the species is very strongly supported (99.7/99/9), but its placement as sister to N. filamentosa n. sp. is only poorly supported (43.9/90/0). The poor support for this placement is linked to the inclusion of NEOP 167, which includes particularly badly fragmented mitochondrial genome assembly data and has the potential for the inadvertent inclusion of cross contaminant fragments, potentially resulting in instability of the placement of this specimen relative to N. filamentosa n. sp... Together, N. orthonema n. sp. and N. filamentosa n. sp. are maximally supported (100/100/100) as sister to N. spironema n. sp.. Both sexes are well represented.

Notes. In all species of Neoperla , micropyles are at the bottom of sulci but in both N. orthonema n. sp. and N. spironema n. sp. they are on top of narrow low ribs ( Fig. 312 ), which apparently are secondarily uplifted sulci.

Etymology. The name is a composite noun in apposition, formed from the Greek stems ortho, straight, and nema, thread, alluding to the straight egg striae and the threads supporting the cap of the anchor.

Figures

FIGURE 491. Codon-model maximum likelihood phylogram based on 83 largely complete mitochondrial genome sequences only (mt-NT). Statistical support values (SH-aLRT, UFBoot) from this and other analyses summarised by squares as explained in the figure. Terminal labels include information on the species, type status, gender, originating country, unique sequence identifier (NEOP###), sequencing approach and total basepairs of 13 mitochondrial, protein-coding genes. Clades of particular interest highlighted with colours that correspond to the clades in other trees. HT = holotype; PT = paratype; PCR = Polymerase Chaine Reaction; WGS = Whole Genome Shotgun.

FIGURE 492. Simplified overview of codon-model maximum likelihood phylogram based on 356 mitochondrial genome and COX1 DNA barcode sequences (all-NT). Terminal labels include information on the species only. Clades of particular interest highlighted with colours that correspond to the clades in other trees. The full tree is shown in Figs 493-498.

FIGURE 496. Partial (clades G, H) codon-model maximum likelihood phylogram based on 356 mitochondrial genome and COX1 DNA barcode sequences (all-NT). See Fig. 493 for common details.

FIGURES 294–304. Neoperla orthonema n. sp.: Male paratype from Lakota, Ivory Coast: 294, 295, abdominal tip; 296, everted penis, with detail from middle of endophallus; 297–299, apex of contracted penis, in dorsal, ventral and lateral views (from left); 300, lateral view of penis apex, beginning eversion (♁ from Kousankoro); 301, female genital situs (vagina flipped back to show the anchor pattern on S8) and 302, egg, Ankasa game prod. res., Ghana; 303, view onto edge of chorion fragment showing variable size of costae (Ivory Coast, Lakota); 304, detail of collar with end of striae (Wli Falls, Ghana). Scales are 100µm. Tergites, sternites and hemitergite 10 are labeled. d, dorsal spines; eb, spines at base of endophallus; sa, spermatheca; SSt, spermathecal stalk; V, vagina; vs, ventral spines.

FIGURES 309–312. Neoperla spironema n. sp.: 309, 310, holotype; 311, a paratype; both from Ankasa Game. Res; 312, SEMdetail of chorion with the 2 kinds of costae and a micropyle (Ivory Coast, USNM; from Moore, 1991). Scales are 100µm, or as indicated. mi, micropyle; sa, spermatheca; SSt, spermathecal stalk; V, vagina.