Trichinella spp

Grzybek, Maciej, Cybulska, Aleksandra, Tołkacz, Katarzyna, Alsarraf, Mohammed, Behnke-Borowczyk, Jolanta, Szczepaniak, Klaudiusz, Strachecka, Aneta, Paleolog, Jerzy, Moskwa, Bożena, Behnke, Jerzy M. & Bajer, Anna, 2019, Seroprevalence of Trichinella spp. infection in bank voles (Myodes glareolus) - A long term study, International Journal for Parasitology: Parasites and Wildlife 9, pp. 144-148 : 145

2.4. Serological screening of Trichinella spp

Bank vole serum samples were analysed using ID Screen ª Trichinella Indirect Multi-species ELISA kit for the detection of anti- Trichinella antibodies (targeting the ES stages of muscle larvae of T. spiralis , T. pseudospiralis , T. britovi and T. nativa ) in animal serum, according to the manufacturer's instructions. Because limited volumes of serum were available, 5 μl of serum were used from each sampled animal. Optical density was measured at a wave length of 450 nm (0.1s) using an ELx800™ Absorbance Microplate Reader (Bio-Tek). Calculation of the concentration of anti- Trichinella antibodies was carried out using KCJr software (Bio-Tek).