Atyaephyra stankoi Karaman 1972

Christodoulou, Magdalini & Kihara, Terue Cristina, 2018, Lectotype designation and distribution updates on the freshwater shrimp species Atyaephyra stankoi Karaman 1972, Zootaxa 4531 (1), pp. 123-133 : 128-130

Atyaephyra stankoi Karaman 1972

( Fig. 3 A–B)

Atyaephyra Desmaresti var. occidentalis Bouvier 1913: 65 –74, Figs 2I , 3I , partim.

Atyaephyra desmarestii desmarestii — Holthuis 1961: 5 –10, Figs 2B , 3B , partim.

Atyaephyra desmarestii stankoi Karaman 1972: 81 –84, Figs 3 , 6, 9, 10 [type locality: Dojransee, Mazedonien].

Atyaephyra stankoi — Christodoulou et al. 2012: 78 –82, Figs 5, 6.

Atyaephyra stankoi — García Muñoz et al. 2014: 415 –416, Fig. 7.

Material examined. Type material. Lectotype: 1 ovigerous female, CL 5.3 mm, ZZDBE-DEC-009, Doiran Lake (= Dojransko jezero), Macedonia, 21.07.1970, leg. M. Karaman [here designated]. Paralectotypes : 20 males, CL 3.0– 4.2 mm, 41 females (6 ovigerous), CL 2.8–5.5 mm, 1 juvenile, ZZDBE-DEC-010, same data collection as holotype . Paralectotypes 4 males, CL 3.2–4.0 mm, 6 females (2 ovigerous), CL 3.2–5.4 mm (including sequenced specimen: Doir 3), OUMNH.ZC-2018-05-029, same data collection as holotype .

Brief description of lectotype ( Fig 3A –B). Rostrum long, slender, dorsal margin slightly curved in the middle and pointed upwards, 7.2 × as long as high, equal to scaphocerite with 26 (2 pre orbital) teeth on dorsal margin of rostrum arranged up to tip and 4 teeth arranged on ventral margin of rostrum. Carapace smooth with pterygostomial angle not protruding, rounded. Pleuron of fifth abdominal segment slightly pointed ending in an about 90° angle. Telson with five pairs of dorsolateral short spiniform setae, distal margin of telson slightly convex with eight spiniform setae. Outermost pair of spiniform setae shortest, similar to dorsolateral setae, adjacent pair stronger terminating beyond the inner six finely setulose setae. Basal segment of antennular peduncle with long stylocerite, with its tip failing to reach distal end of basal segment. Anterolateral lobe of basal segment short and rounded. Basal endite of first maxilliped reaching before distal end of exopod. Distal one-third of terminal segment of third maxilliped bearing 21 mesial setae and one subdistal lateral spine near the base of larger terminal spine. Eggs size 0.50 x 0.35 mm.

Distribution. Atyaephyra stankoi is a Balkan endemic species found in freshwater habitats in the mainland Greece, spanning from south Western Greece northwards to Central Macedonia, and to F.Y.R.O.M., spanning from the type locality (Doiran Lake) to north of Skopje ( Fig. 4 ).

Remarks. According to the original description of A. stankoi by Karaman (1972), the type material consisted of 27 male and 50 female individuals, the type locality was Dojransee (German for Doiran Lake), Mazedonien (= Macedonia) and the collector was himself. The current syntypic material came to the attention of the authors after Dr Ivo Karaman’s son of Mladen Karaman, indication. The material was kept within the zoological collections of the University of Novi Sad, Serbia and is consisted of 24 males, 48 females and 1 juvenile, 4 individuals are missing. However, on the actual label ( Fig. 3C ) of this material the following information is provided: (1) “ Atyaephyra desmaresti spp. stankoi n.ssp. ”, (2) “ Syntypen!”, (3) “Dojransko jezero” (Serbian for Doiran Lake) and (4) “ 21-vii-70 ”. Although the number of individuals is slightly less (could be that very smallsized individuals were lost) the collector and the locality completely agree with those mentioned in Karaman (1972). In the original description by Karaman (1972) the date of the collection was not given, but the collection date of these specimens is earlier than the published date of the original description, so there is no contradiction on this point. There is no doubt that the ZZDBE-DEC-009–010 and the OUMNH.ZC-2018-05-029 specimens are those upon which Karaman’s (1972) original description was based, especially since they are labelled as syntypes. Thus in order to be in accordance with the International Code of Zoological Nomenclature (ICZN) and specifically in accordance with the Article 75.8 (ICZN 1999) the rediscovered material becomes again the name-bearing type and the neotype (NHM 2012.1475, adult female, CL 6.0 mm) designated by Christodoulou et al. (2012: 77) is set aside. The fittest (with most of its appendages intact) and one of the largest ovigerous females is hereby designated as a lectotype while the remaining material is set as paralectotypes according to Article 74 (ICZN 1999). The lectotype and the majority of the paralectotypes are stored in the zoological collections of the University of Novi Sad, Serbia, while 10 paralectotypes are donated to the OUMNH for storing for safety reasons. No change is deemed for the type locality as the neotype was also collected from Doiran Lake.

The COI gene tree produced by Neighbour-joining analysis confirmed the placement of the studied individuals to the species Atyaephyra stankoi . All specimens classified as A. stankoi by morphology including one of the paralectotypes as well as the sequences mined from GenBank ( García Muñoz et al. 2014) were cluster together with high support ( Fig. 2 ). Three phylogenetic clusters were recognised within the species A. stankoi in the COI tree ( Fig 2 ). First cluster includes specimens from lakes and rivers in Western Greece, the second from Attiki and the third from Thessaly and Central Macedonia. The uncorrected p-distances of the COI mitochondrial sequences range between the specimens of A. stankoi from 0.0–4%, while between A. stankoi and the other European Atyaephyra species range from 9.3–17.29%. The total number of A. stankoi ’s haplotypes obtained from 14 collection sites was 10 (H9–H18). Three haplotypes (H9, H10, H16) were shared between other specimens from adjacent localities ( Table 1, Fig. 2 ). Additionally, eight new haplotypes belonging to other European Atyaephyra species are given for comparison purposes. In the current study the species is recorded for the first time from Pineios River in Peloponnesus ( Table 2; Fig. 4 , collection site: 7) and the Kifisos River in Attica ( Table 2; Fig. 4 , collection site: 9).

Figures

FIGURE 2. Neighbour-Joining tree of p-distance based on COI sequences of Atyaephyra stankoi Karaman 1972 and other European Atyaephyra species. Specimen codes, sampling sites numbers and respective haplotypes are indicated in each branch.

FIGURE 3. A, Lectotype of Atyaephyra stankoi Karaman 1972 (ZZDBE-DEC-009) with antennal flagella. B, Enlargemnt of main body of lectotype. Scale 2 mm. C, Labels of syntypic material of Karaman (1972) deposited at the Zoological collections of the University of Novi Sad, Serbia.

FIGURE 4. Map showing the distribution of Atyaephyra stankoi Karaman 1972 based on field collections, museum collections and literature data. Red lines indicated watershed boundaries. Black lines indicate country boundaries. Numbers indicate collection sites reported in the literature or in the current study (see Table 2).