Apiospora pseudosinensis (Crous) Pintos & P. Alvarado, Fungal Systematics and Evolution 7: 207. (2021)

Liu, Rongyu, Li, Duhua, Zhang, Zhaoxue, Liu, Shubin, Liu, Xinye, Wang, Yixin, Zhao, Heng, Liu, Xiaoyong, Zhang, Xiuguo, Xia, Jiwen & Wang, Yujiao, 2023, Morphological and phylogenetic analyses reveal two new species and a new record of Apiospora (Amphisphaeriales, Apiosporaceae) in China, MycoKeys 95, pp. 27-45 : 27

Apiospora pseudosinensis (Crous) Pintos & P. Alvarado, Fungal Systematics and Evolution 7: 207. (2021)

Fig. 4

Arthrinium pseudosinense ≡ Arthrinium pseudosinense Crous, in Crous & Groenewald, IMA Fungus 4(1): 148 (2013).

Description.

Asexual morph: On WA, hyphae 1.1-2.9 μm diam., hyaline, branched, septate. Conidiophores cylindrical, septate, verrucose, flexuous, sometimes reduced to conidiogenous cells. Conidiogenous cells globose to subglobose, erect, blastic, aggregated in clusters on hyphae, hyaline to pale brown, smooth, branched, 9.4-11.0 × 6.1-8.8 μm, mean ± SD: 10.4 ± 0.7 × 7.7 ± 1.1 μm (n = 40). Conidia globose, subglobose to lenticular, with a longitudinal germ slit, occasionally elongated to ellipsoidal, brown to dark brown, smooth to finely roughened, 7.5-11.5 × 7.0-9.5 μm, mean ± SD: 10.1 ± 1.3 × 8.3 ± 0.6 μm, L/W = 1.1-1.3 (n = 40). Sexual morph: Undetermined.

Culture characteristics.

Colonies on PDA flat with irregular margin, aerial mycelium white to pale yellow, floccose cottony; reverse pale yellow to yellow. PDA attaining 69.5-78.5 mm in diameter after 7 days at 25 °C, growth rate 9.5-11.5 mm/day.

Specimens examined.

China, Shandong Province: Dongying Botanical Garden , on diseased leaves of bamboo, 15 July 2022, R.Y. Liu, HSAUP 0221, living culture SAUCC 0221 ; China, Hainan Province: Diaoluoshan National Nature Reserve , on diseased leaves of bamboo, 29 June 2021, R.Y. Liu, HSAUP 0022, living culture SAUCC 0022 .

Notes.

Apiospora pseudosinensis was originally described from bamboo leaves collected in the Utrecht Botanical Garden of the Netherlands ( Crous and Groenewald 2013; Pintos and Alvarado 2021). In the present study, DNA sequences obtained from two strains (SAUCC 0221 and SAUCC 0222) collected also from bamboo leaves, were not significantly different from those of A. pseudosinensis (Fig. 1 ). Morphologically, our strains were similar to the original description (conidia 8-10 × 7-10 μm diam. in surface view, 7-8 μm diam. in side view). We therefore consider the newly found strains as A. pseudosinensis ( Crous and Groenewald 2013; Pintos and Alvarado 2021).

Figures

Figure 1. Phylogram of Apiospora based on combined ITS, LSU, tef 1 and tub 2 genes. ML bootstrap support values (MLBS ≥ 50 %) and Bayesian posterior probability (BYPP ≥ 0.90) are shown as first and second position above nodes, respectively. Strains from this study are shown in red, ex-type or ex-epitype cultures are indicated in bold face. Some branches were shortened according to the indicated mulipliers.

Figure 4. Apiospora pseudosinensis (SAUCC 0221) a leaf of host plant b, c surface (b) and reverse (c) sides of colony after incubation for 7 days on PDA d conidiomata formed in culture e, f conidiogenous cells and conidia g, h conidia. Scale bars: 10 μm (e-h).