Musonycteris harrisoni, DNA

Basaldúa, Sonia, Rodriguez-Pilco, Milena, Gutiérrez-Guerrero, Yocelyn, Ortega, Jorge & Baeza, J. Antonio, 2023, The mitochondrial genome of the endemic and endangered trumpet-nosed bat Musonycteris harrisoni (Chiroptera: Phyllostomidae), Journal of Natural History 57 (21 - 24), pp. 1206-1219 : 1208

Sampling of Musonycteris harrisoni, DNA extraction, and sequencing

A muscle tissue sample of Musonycteris harrisoni (specimen ID: GHC216; catalogue number: 16,153) was donated by the Museum of Zoology ̍Alfonso L. Herrera̾, Facultad de Ciencias, Universidad Nacional Autonoma de Mexico, Mexico to the ̍Laboratorio de Bioconservación y Manejo̾, Instituto Politécnico Nacional, Escuela Nacional De Ciencias Biológicas (ENCB), Mexico City, Mexico. The specimen was originally collected in Palma de Cayaco (17°13̾45.5″N, 100°35̾04.6″W), Tecpan de Galena, Guerrero, Mexico.

Genomic DNA (gDNA) was isolated using the DNA Blood and Tissue Kit (Qiagen, Germany) following the manufacturer̾s instructions and shipped to the ̍Vincent J. Coates̾ Genomics Sequencing Laboratory, University of California, Berkeley, for sequencing in a Illumina HiSeq 4000 platform. A total of 203,048,998 pair-end (PE) reads (150 bp) were generated by the facility and made available as fastq files. The totality of these reads was used for mitochondrial genome assembly.