Trichinella spp
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publication ID |
https://doi.org/ 10.1016/j.ijppaw.2023.01.003 |
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persistent identifier |
https://treatment.plazi.org/id/F842879B-BD18-C068-D123-FD46FBF89843 |
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treatment provided by |
Felipe |
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scientific name |
Trichinella spp |
| status |
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2.3. Screening for Trichinella spp
All raccoons were analyzed for Trichinella spp . muscle larvae using pepsin digestion. At least 50 g of tissue derived from skeletal muscles (tongue, masseter, diaphragm, and forelimb muscles; approx. 10 g each) were digested in 250 ml of a solution containing 1:100 diluted HCl (37% HCl; Carl Roth GmbH, Germany) and 1% pepsin (Merck, Darmstadt, Germany) while stirring at 35–38 ◦ C until meat particles dissolved (approximately 45 min). Afterwards, the solution was centrifuged at 1500g for 5 min. The supernatant was discarded and the pellet resuspended in physiological saline solution. The resuspension was microscopically examined for the presence of Trichinella spp . larvae at 40–100 times magnification. For additional testing of Trichinella spp ., we used the PrioCHECK™ Trichinella Alternative Artificial Digestion (AAD) Kit (Thermo Fisher Scientific, Langenselbold, Germany) according to the manufacturer’ s protocol.
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