Boletellus putuoensis N.K. Zeng, Yi Li, Chang Xu

Cho, Minseo, Kwon, Sun Lul, Jang, Seokyoon, Yoo, Yeonjae, Lee, Sang Hyun, Kwon, Dae Young, Kim, Changmu, Lim, Young Woon & Kim, Jae-Jin, 2024, Notes of 15 unrecorded macrofungi in Korea, Journal of Species Research 13 (1), pp. 67-88 : 73

Boletellus putuoensis N.K. Zeng, Yi Li, Chang Xu ,

Xu Zhang & J.R. Wang, Phytotaxa 554(2): 156 (2022) [MB#571000] ( Figs. 1C and 2C )

Korean name: DZǙȐƎāfflŷ

Pileus convex, margin straight, even, 40-50 mm in diam. Pileus surface smooth, partially cracked, brown, margin dusky red. Pore surface yellowish brown when young, becoming dark brown when old, round to angular pores, 1-2 per mm. Stipe central, equal, 65 × 5 mm, surface smooth, concolorous with pileus. Hyphal system monomitic, generative hyphae simple septate, rarely branched, without clamp connections, thin-walled, hyaline, 3.3-8.4 μm. Basidia obovoid to pyriform, 4-spored, smooth, thin-walled, hyaline, without a clamp connection, 21.9-30.0 × 10.5-14.2 μm. Cystidia fusiform to lageniform, smooth, thin-walled, hyaline, without a clamp connection, 48.8-75.7 × 10.3-13.3 μm. Basidiospores ellipsoid to cylindrical, striate, with 5-8 ridges, thick-walled, yellowish brown to reddish brown, bearing a guttule, 10.9-14.0 × 6.3-9.6 μm [Q = 1.45-1.73].

Specimen examined. Korea, Gyeongsangbuk-do, Cheongsong-gun, Juwangsan National Park, 36°23 ʹ 43.9 ʺ N, 129° 09 ʹ 15.0 ʺ E, 348 m, mixed hardwood forest, occurring solitary on the ground near Yongchu Falls , 31 Jul 2019, S. Jang and S. L. Kwon, KUC20190731-68 (NIBRFG0000 506145) .

Notes - Boletellus Murrill includes about 64 species worldwide from tropical to temperate regions ( Murrill, 1909; Singer, 1970; Snell and Dick, 1970; Corner, 1972;

Watling and Li, 1999; Bessette et al., 2000; Watling, 2001; Halling and Mueller, 2005; Horak, 2011; Zeng and Yang, 2011; Sato et al., 2012; Bessette et al., 2017; Tibpromma et al., 2017; Parihar et al., 2018). Among them, nine species are recorded in Korea ( Cho and Park, 1988; An, 1998). Boletellus putuoensis is characterized by small-sized brownish pileus, fusiform and lageniform cystidia, and thick-walled striate basidiospores ( Xu et al., 2022). Additional observation using SEM is required to observe the surface of basidiospores clearly. The original description differs from KUC20190731-68 by a smaller pileus (17-48 mm) and observance of fusiform cystidia only ( Xu et al., 2022). According to the phylogenetic analysis, Bo. chrysenteroides is the most closely related to Bo. putuoensis ( Fig. 5 ). However, Bo. chrysenteroides has narrower basidiospores (12-16 × 4.6-7.5 μm) ( Smith, 1971). Boletellus putuoensis was previously reported from Southeastern China, Guangdong, Hainan, and Zhejiang, which have subtropical to tropical climate ( Xu et al., 2022; Xue et al., 2023). This study is the first report of Bo. putuoensis in the temperate region, over 30 degrees north latitude.

Figures

Fig. 1. Macro-morphological characteristics of fifteen unrecorded macrofungi in Korea. A. Agaricus thiersii; B. Baorangia alexandri; C. Boletellus putuoensis; D. Entoloma bulakhae; E. Entoloma pygmaeopapillatum; F. Entoloma subtenuicystidiatum; G. Gerronema kuruvense; H. Hyphoderma nudicephalum; I. Hyphoderma tenue; J. Macrolepiota subcitrophylla; K. Mycena jingyinga; L. Mycena yuezhuoi; M. Ophiocordyceps vespulae; N. Scytinostroma acystidiatum; O. Steccherinum straminellum (scale bar=10 mm for A-D, G-J, M-O; 5 mm for E, F, L; and 2.5 mm for K).

Fig. 2. Micro-morphological characteristics of fifteen unrecorded macrofungi in Korea. A. Agaricus thiersii; B. Baorangia alexandri; C. Boletellus putuoensis; D. Entoloma bulakhae; E. Entoloma pygmaeopapillatum; F. Entoloma subtenuicystidiatum; G. Gerronema kuruvense; H. Hyphoderma nudicephalum; I. Hyphoderma tenue; J. Macrolepiota subcitrophylla; K. Mycena jingyinga; L. Mycena yuezhuoi; M. Ophiocordyceps vespulae; N. Scytinostroma acystidiatum; O. Steccherinum straminellum(scale bars=10μm).

Fig. 5. The phylogenetic tree of Boletellus putuoensis(KUC20180907-19). The tree was constructed based on ITS gene datasets of the genus Boletellus. Aureoboletus venustus was used as an outgroup. The newly generated sequence is shown in blue and bold. Bootstrap support values more than 70 are shown.