New observations on Myxozoa of the goldline sea bream Sarpa salpa L. 1758 (Teleostei: Sparidae) from the Mediterranean coast of Tunisia Laamiri, Sayef Zootaxa 2014 3887 2 157 190 Thelohan, 1892 Thelohan 1892 [151,597,1726,1752] Myxosporea Ceratomyxidae Ceratomyxa Animalia Bivalvulida 2 159 Myxozoa species arcuata    Typehost:  Gaidropsarus mediterraneus(syn.  Motella tricirrata), shore rockling ( Gadiformes: Gadidae).  Other hosts:  Pagellus bogaraveo(Brünnich, 1756)(syn.  P. centrodontus) blackspot sea bream;  Callionymus lyra(Linnaeus, 1758)dragonet;  Chromis  chromis(Linnaeus, 1758)(syn.  Heliases chromis) damselfish;  Crenilabrus melops(Linnaeus, 1758)corkwing wrasse;  Dicentrarchus labrax(Linnaeus, 1758)Mediterranean sea bass;  Gadus morhua(Linnaeus, 1758)Atlantic cod;  Gobius paganellus(Linnaeus, 1758)rock goby;  Merlangius merlangus(Linnaeus, 1758)whiting; Parophidium  vasalli(Risso)(syn.  Ophidium vasalli);  Sarpa salpa(Linnaeus, 1758)goldline sea bream;  Scorpaena scrofa(Linnaeus, 1758)red scorpionfish;  S. porcus(Linnaeus, 1758)black scorpionfish;  S. notata(Rafinesque, 1810)small red scorpionfish;  Uranoscopus scaber(Linnaeus, 1758)Atlantic stargazer.   Typelocality:Roscoff, Atlantic coast of France.  Other localities:Northeast Atlantic, Black Sea, Adriatic Sea, Mediterranean Sea, Gulf of Tunis, Tunisia( 36°45’N, 10°15’E).  Site of infection:Within gall bladder  Prevalence:the overall prevalence is 4.5% (15/330) ( Fig. 9). None fish 0% (0/120) recolted from Bay of Bizerte, was infected with  C. arcuata. The infection was confined only at Gulf of Tuniswith prevalence 7.14% (15/ 210) distributed as following, 03/2012: 0% (0/30); 04/2012: 0% (0/30);05/2012: 10% (3/30); 06/2012: 10% (3/30); 07/2012: 16.7% (5/30); 08/2012: 0% (0/30); 05/2013: 15% (3/20); 06/2013: 10% (1/10) (see Table 4).  Mean intensity:70.2 ± 14.6 spores/infected fish (+++++) ( Fig. 10) (see Table 4).  Type-material:Digitized photos of syntypespores were deposited in the parasitological collection of the Museum National d’Histoire Naturelle ( MNHN), Paris, Coll. No. ZS 116. Description  Vegetative stages.Polymorphous but generally club-shaped. Only one post-mature disporic plasmodium was seen in this study when the spores were being separated ( Fig. 1A).  Spores(n = 30 fresh spores). Mature spores were arcuate in sutural view ( Figs. 1A–E, 8A) and elongated in apical view ( Fig. 1H), measuring 7.5 ± 0.4 (7–9) Μm in length and 35.6 ± 3.3 (30–40) Μm in thickness. Posterior angle was concave 150.6 ± 4.2 (142–156°). Valves equal slightly tapering with rounded ends and smoothly ovoid in lateral view ( Fig. 1G). Straight sutural line visible between the two valves. A binucleate sporoplasm, with several sporoplasmosomes, granular in which remnants of valvogenic nuclei persist at tips of the shell valves ( Figs. 1F,8A). Polar capsules were subspherical to pyriform, 3.3 ± 0.4 (3–4) Μm in length and 3 ± 0.4 (2.5-3.5) Μm in width (n = 30). Four to five coils of polar filaments could be observed inside the polar capsules. Remarks According to the available literature,  C. arcuata Thélohan, 1892was identified as the first typical species described from the myxosporean genus  Ceratomyxa. It was originally reported from several hosts in different families of fishes from Atlantic and Mediterranean off Franceand Mediterranean off Italy( Kudo 1920). Until now, in addition to the present work,  C. arcuata Thélohan, 1892has been described in 10 different families of fish ( Callionymidae, Gadidae, Gobiidae, Labridae, Moronidae, Ophidiidae, Scorpaenidae, Sparidae, Pomacentridae, Uranoscopidae) ( Kalavati & Mackenzie 1999). This confirm that  C. arcuatais euryxenous, infecting a huge range of unrelated hosts. However, Kalavati & Mackenzie (1999)believed that some host could be infected accidently by  C. arcuata Thélohan, 1892due to weak intensity and prevalence of infection. Nevertheless, Mackenzie et al. (2005) revealed that  C. arcuatacan be suitable as biological tags for stock discrimination of some fish species. Moreover, this myxosporean has the most worldwide distribution. It has been reported from different areas around the world as the Northeast Atlantic and a record of 15 different fish species has been infected with  C. arcuata(Thélohan 1892,1895; Dunkerly 1921; Théodoridès 1955; Noble 1957; Kabata 1967; Shotter 1970; Feist & Ronga 1996; Kalavati & Mackenzie 1999; Mackenzie et al. 2005), Mediterranean Sea ( Thélohan 1892, 1895; Parisi 1912; Jameson 1913; Georgèvitch 1916; Present study), Black Sea ( Zaika 1966) and Adriatic Sea ( Lubat et al. 1989). The absence of reports of  C. arcuatafrom the Northwest Atlantic suggests that this species is restricted to temperate waters of the Northeast Atlantic ( Kalavati & Mackenzie 1999; Mackenzie et al. 2005). Historically, the difference in the published descriptions suggested the possibility to include more than one species of  Ceratomyxa. On the basis of the presence or absence of small refringent globules in the trophozoite and the size of the pseudopodia, Labbé (1899)divided  C. arcuatainto two subspecies:  C. arcuata typica, from  P. bogaraveo,  C. melopsand  G. mediterraneusand  C. arcuata scorpaenarum, from  S. scrofaand  S. porcus, but this division was rejected by Parisi (1912)and Kudo (1920)as being too arbitrary. Both Parisi (1912)and Meglitsch (1960), noted in their descriptions that the specimens of  C. arcuatawere smaller with valves of unequal length. Mackenzie et al. (2005) declared that the differences between the description of Meglitsch and both the original description of  C. arcuataand the redescription of Kalavati & MacKenzie (1999)suggested that Meglitsch was mistaken in his identification of “  C. arcuata” from the serranid  Anthias punchellasfrom New Zealandwaters. The author is in perfect agreement with this declaration since the comparison between the measurements of the current species and the species identified by Meglitsch (1960)shows no similarity neither in shape nor in size. In this study, the features characteristic of  C. arcuatawith both sporogonic stages (Disporous) and the mature spores (Equal valves) are in agreement with the original description of Thélohan (1895)and the study of Kalavati & Mackenzie (1999). Likewise, this study records the goldline sea bream  S. salpa(L.) as a new host of  C. arcuatafrom the family of sparidaefrom the Mediterranean Sea after the blackspot sea bream  P. bogaraveo(Brünnich, 1756). Ecological notes In this study, based to the classification of parasites by Valtomen et al. (1997),  C. arcuatahas a parasitic status as scarce species (P <10%). Infection by  C. arcuatawas confined only in Gulf of Tunis. During the sampling period, the highest prevalence of infection was noted in July 16.7% (see Table 4) and mean intensity was moderate with 70.2 ± 14.6 spores per infected fish ( Fig. 10).