The Dixa inextricata Dyar & Shannon (Diptera: Dixidae) species group, with two new cryptic species from the eastern Nearctic Region Moulton, John K. Zootaxa 2016 4121 4 458 472 9HV7K [151,336,1758,1784] Insecta Dixidae Dixa Animalia Diptera 5 463 Arthropoda species calciphila sp. nov.    Typematerial.  HOLOTYPE♂, labeled: “  USA: TN: MARION COUNTY/ Spr @ US64/TN41 opposite Raccoon Mtn/ N35°03′31″ W85°29′08″, 243 m/ 24.iv.2014/ J.K. Moulton”; “ HOLOTYPE/ Dixa/ calciphila/ J.K. Moulton [red label]” ( CNC).  PARATYPES: Same data as holotype, 5♂ and 2♀ each to CNCand USNM.  Additional material examined.  USA: KENTUCKY: EDMONSON COUNTY, Mammoth Cave NP, Cinnamon Fern Bog, UV light trap, 7.vi.2007, C. Parker & J. Robinson (1♂, USGS); Thru Sprs, UV light trap, 15– 16.viii.2006, C. Parker & J. Robinson (3♂, USGS).  HARTCOUNTY, Three Hundred Sprs nr Munfordville, N37°17′29.85″ W85°43′45.70″, 29.ix.2008, JKM—1♂. Unnamed trib Lynn Camp Crk, 10.iv.2008, JKM—1♂. TAYLOR COUNTY, Little Angel Spr, Trib Craig’s Crk, N of Campbellsville, N37°26′40″ W83°21′18″, 9.vi.2005, JKM; 1.vii.2004, Malaise trap, G.K. Weddle—2♂. 17.iv.2005, JKM—1♂, 1♀, 1L; 15.ix.2005—1♂. TENNESSEE: FRANKLIN COUNTY, Trib TurkeyCrk, ex TN Rt 16 (Old Turnpike Rd.), Walls of Jericho SNA, N34°59′40.77″ W86°03′38.98″, 23.v.2008, JKM—1♂. MARION COUNTY, Spr @ US64/TN41 opposite Raccoon Mtn, 243 m, N35°03′31″ W85°29′08″, 24.iv.2014, JKM—20♂, 7♀; 4.ix.2015—5♂, 2♀. Daniel Cave Spr, N36°26′00″ W85°48'00.00″, 8.ix.2010, JKM—3♂. MORGAN COUNTY, Frozen Head SP, Flat Frk, N36°07′10.91″ W84°27′52.67″, 20.vi.2014, JKM—1♂. Obed Wild and Scenic R NP, Melton Mill Br @ Footbridge, Point Tr. below Lilly Bluff Overlook, 357 m, UV light trap, 6.ix.2005, C. Parker (2♂, USGS). PUTNAM COUNTY, Tayes Hollow Spr. @ TN Rt. 84, S of Monterrey, 498 m, N36°08′12″ W85°17′28″, 17.iv.2010, JKM—1♂, 2♀, 1L; 7.iii.2009—4♂ & 2♀; 10.iii.2009—3♂, 1♀, 6L; 31.iii.2013—6♂, 4♀, 4L, 2P.   FIGURES 9–11. Male terminalia, lateral view, in non-rotated (dorsal-ventral) orientation. 9,  Dixa appalachiensis  sp. nov.; 10,   D. calciphila  sp. nov.; 11,  D. inextricata.Scale bar = 50 µm.   Diagnosis. This species can be distinguished from  D. appalachiensis  sp. nov.and  D. inextricataby the following characters: Morphological. Ventral margin of gonostylus strongly convex; basal gonocoxal lobe with apex noticeably acuminate; cercus narrow, acuminate, as long as lateral width of proctiger. Molecular. Among the most significant differences within the 66 nucleotides (22 codons) shown ( Fig. 38) that serve to distinguish this species from the other two are amino acid substitutions at the following codon positions: 2 (valine/methionine), 3 (alanine/proline), 14 (asparagine/serine), 18 (asparagine/serine), 19 (arginine-CG)/arginine-AG or lycine), and 21(glycine/absent).   Description.  Male( Fig. 3). Same as  D. appalachiensis  sp. nov., except as follows: Wing length ( n=13) 2.6– 3.1 (avg=2.9) mm. Wing darker smoky gray. Thoraxgenerally darker brown ( Fig. 3), most distinctly differing in katepisternum concolorous with head, anepisternum, and anepimeron and contrasting sharply against whitish to straw-colored mesocoxa. Meron brown, nearly as dark as katepisternum. Terminalia( Figs 10, 15–17, 23): 9S lightly sclerotized, with straight anterior margin. Proctiger heavily sclerotized ( Figs 15–17, 23). Cercus narrow, acuminate, as long as lateral width of proctiger ( Figs 10, 15). Basal gonocoxal lobe directed posterodorsally in lateral view with apex distinctly falcate ( Fig. 10), appearing rectangular in ventral ( Fig. 16) and terminal views ( Fig. 17). Apical gonocoxal lobe blunt, directed mediolaterally, in lateral view appearing ca. 1/ 5Xor less of gonostylus length ( Figs 10, 15). Gonostylus triangular in lateral view, ca. 1.3–1.5X longer than greatest depth, ventral margin noticeably convex, bulbous ( Fig. 10).  Female( Fig. 4). Wing length ( n=4) 2.9–3.2 (avg=3.0) mm. Not reliably separated from  D. appalachiensis  sp. nov.or  D. inextricata.   Etymology. Named for the karst geography ( calc- = lime + - phil= loving) that largely defines the region in which it occurs.   Distribution. Currently known only from Kentucky and Tennessee ( Fig. 37).   Remarks. This species is known from streams ranging from small first order hillside trickles ( Fig. 33) to raging torrents ( Fig. 34) originating from underground streams. In central Tennessee adults are already on the wing by early March, earlier than other dixids found in the region. This species has been collected in association with  Dixa fusca,  D. modestasensu Peters,  Dixanr. repandaPeters, and a member of the  Dixella indianacomplex. This species is expected to occur in northeastern Alabama and possibly, southern Indiana. Morphologically and molecularly, this species is more distantly related to the other two species in the group than they are to one another.