Hemigrammus lunatus Durbin in Eigenmann, 1918: 164 Hyphessobrycon maxillaris Fowler, 1932: 354 Hemigrammus maxillaris Weitzman, 1985: 808 A new species of Hemigrammus Gill, 1858 (Characiformes: Characidae) from the rio Madeira and rio Paraguai basins, with a redescription of H. lunatus Ota, Rafaela P. Lima, Flávio C. T. Pavanelli, Carla S. Neotropical Ichthyology 2014 2014-06-30 12 2 265 279 3KNZ7 Durbin, 1918 Durbin in Eigenmann 1918 [925,1335,526,551] Actinopterygii Characidae Hemigrammus Animalia Characiformes 5 270 Chordata species lunatus    Figs. 4-7      Hemigrammus lunatus Durbin in Eigenmann, 1918: 164[ typelocality: “ Amazonbasin”, restricted herein to “ Brazil, Amazonas, Codajás, lago Badajós( ca. 03º21’S 62º41’W)”; see below; “ Obidos”, “ Caceres”, “ Jauru”].     Hyphessobrycon maxillaris  Fowler, 1932: 354[ typelocality: “ Descalvados”, rio Paraguai basin, Mato Grosso, Brazil].    Hemigrammus maxillaris.  Weitzman, 1985: 808[new combination].   Diagnosis.  Hemigrammus lunatusdiffers from most congeners, except from  H. barrigonae,  H. machadoi, and  H. ulreyi, by possessing a wide dark horizontal stripe across the eye ( vs. Distribution.  Hemigrammus machadoiis known from the upper rio Paraguaibasin, from the rio Guaporé and some other smaller tributaries of the rio Mamoré and rio Madeira, on the upper and middle portions of the rio Madeira basin in Brazil( Fig. 3). Comparisons between  Hemigrammus machadoispecimens from rio Guaporé, rio Pacaás Novos and upper rio Paraguaibasins revealed no differences in morphometric, meristics or color pattern, and the data of these distinct populations are pooled together in the description and Table 1.  Ecological notes.Specimens of  Hemigrammus machadoiare typically found in slow flowing, low-gradient streams and small rivers, with clear, sometimes slightly dark-stained waters, as the upper rio Guaporé and the typelocality, the igarapé Barreiro. River bottom at those sites was constituted mainly of sand and clay. Aquatic vegetation was abundant at the igarapé Barreiro and at some sites at the rio Guaporé and  H. machadoiseemed to prefer its surroundings. The species was also captured in clear water wetlands, adjacent to the rio Guaporé. Analyses of dissected specimens (MZUSP 95365) collected in November revealed two mature females, 26.6-27.2 eye stripes absent or, when present, vertical in the remaining congeners). It can be distinguished from most congeners, with the exception of  H. barrigonae,  H. boesemani,  H. geisleri,  H. machadoi,  H. mimus, and  H. ulreyi, by the possession of a well defined narrow dark stripe at the basis of the anal fin ( vs. dark stripe at the basis of anal fin absent).  Hemigrammus lunatuscan be easily distinguished from  H. boesemani,  H. geisleri, and  H. mimus, by possessing a distinct roundish dark humeral blotch ( vs. humeral blotch absent) and by lacking a blotch on caudal peduncle or any distinct patch of pigmentation on caudal fin ( vs. blotch on caudal peduncle present in  H. boesemaniand  H. geisleri, a dark marking present at the basis of each caudal-fin lobe basis in  H. mimus).  Hemigrammus lunatuscan be distinguished from  H. barrigonaeand  H. ulreyiby lacking a narrow, well-defined longitudinal dark stripe ( vs. present), and by lacking a discrete blotch on caudal peduncle ( vs.present in  H. barrigonae) or a dark pigmentation patch on the basis of anteriormost dorsalfin rays ( vs. present in  H. ulreyi). It can be diagnosed from  H. machadoi, the most similar congener by having a small roundish dark humeral blotch, extending horizontally from fourth through sixth lateral-line scales, and vertically from fourth through fifth scale rows above lateral line ( vs.conspicuous, vertically elongated humeral blotch, extending horizontally from second through sixth lateral-line scales, and vertically from third row above lateral line to first row below it), and by having 6-7 gill-rakers on upper branch and 11-12 on lower ( vs. 4-5, and 9-10, respectively). Additionally, the lower number of cusps of inner premaxillary, dentary, and maxillary largest tooth (5 vs. 5-7 cusps, mode 7, in premaxillary and dentary; 1-3 vs. 3-5, mode 5, in maxillary), and total vertebrae (32-33 vs. 34-35) helps to distinguish  H. lunatusfrom the new species.   Description.Morphometric and meristic data summarized in Table 2. Body compressed, moderately elongated; greatest body depth located anteriorly to dorsal-fin origin. Dorsal profile of head convex from tip of snout to anterior naris, straight to slightly concave from latter point to tip of supraoccipital spine. Dorsal profile of trunk moderately convex from tip of supraoccipital spine to dorsal-fin origin; inclined from latter point to adipose-fin origin and slightly concave along caudal peduncle. Ventral profile of body convex from tip of lower jaw to anal-fin origin; inclined along anal-fin base. Ventral profile of caudal peduncle slightly concave. Jaws equal, isognathous; mouth terminal. Maxillary slightly curved; posterior terminus surpassing vertical through anterior margin of eye. Small ossification anterior to first unbranched ray present in all 11 c&s specimens examined. Distal margin of dorsal fin straight. Dorsal-fin origin at midbody or slightly behind this point; base of posterior most dorsal-fin ray slightly behind vertical through anal-fin origin. First dorsal-fin pterygiophore insertion posterior to neural spine of tenth vertebra. Adipose fin small. Pelvic-fin origin ahead of vertical through dorsal-fin origin; tip of longest ray surpassing anal-fin origin. Anal fin falcate, last unbranched ray to fourth branched ray longest, with remaining rays decreasing gradually in size towards anal-fin end; last anal-fin pterygiophore insertion behind hemal spine of fifteenth caudal vertebrae (11). Caudal-fin scales covering about one-third of upper and lower caudal lobes, mainly arranged on upper and lower caudal lobes margins, gradually decreasing in size. Caudal fin bifurcated; lobes slightly pointed, almost equal in size, inferior lobe slightly longer than superior. Precaudal vertebrae 12-13; caudal vertebrae 20(11); total vertebrae 32(5) or 33(6). Supraneurals 3(3), 4(7), or 5(1). Branchiostegal rays 4(11).  Color in alcohol.Overall ground coloration of body light tan. Anterior portion of lower jaw, snout, and dorsal portion of head with dense concentration of small dark chromatophores, imparting an overall dark color. Gular area and infraorbitals clearer; third infraorbital and opercle silver in specimens retaining guanine pigmentation. Tip of maxillary, opercle and fourth and fifth infraorbital with scattered, relatively large dark chromatophores. Eye with broad dark longitudinal midlateral stripe (not discernible in specimens retained for a long period in formalin). Longitudinal dark stripe along midline of body present, very faint and narrow, originating after dark humeral blotch and extending up to approximately vertical through middle of caudal peduncle or slightly behind this point. Scales from dorsal region of body posteriorly bordered with dark chromatophores, conferring a moderately developed reticulate pattern. Dark humeral blotch conspicuous, small, roundish, with well-defined contour, extending from fourth through sixth lateral-line scales, and vertically from fourth through fifth scale rows above lateral line. Dark chromatophores arranged along margins of hypaxial muscles bundles from area above anal fin to caudal peduncle. Dorsal fin mostly hyaline, with few dark chromatophores concentrated along unbranched rays and third branched rays and its interradial membranes. Adipose, pectoral and pelvic fins almost hyaline, with few dark chromatophores scattered mainly along their distal margins.Anal fin hyaline, with few dark chromatophores usually concentrated on its unbranched rays and interradial membranes, forming a dark narrow margin. Anal fin with narrow, conspicuous dark stripe along anal-fin base. Caudal fin hyaline, its main external rays with few dark chromatophores along their margin. Caudal spot absent ( Fig. 7).   Fig. 5.  Hemigrammus lunatus, NUP 2112, 35.9 mm SL, Brazil, Mato Grosso State, Rosário Oeste, córrego Imbaúba, tributary of rio Cuiabá, rio Paraguai basin.  Color in life.Based on a picture of a freshly collected specimen from Manso Reservoir, upper rio Paraguaibasin, and freshly collected specimens from the rio Amazonas near Santarém, Pará (ZUEC 7995). Overall coloration pattern light yellow or clear. Gular region, opercle, infraorbital bones, and abdominal with silver hue. Tip of dentary, snout and top of head light brown, with yellow tinge. Dorsal, pelvic, anterior portion of anal and caudal fins, and adipose fin yellow to light orange, translucent in the specimens from rio Amazonas. Dark midlateral stripe faint, plumbeous, extending into caudal peduncle ( Fig. 5).  Sexual dimorphism.As previously mentioned by Lima& Sousa (2009: 162), mature males of  Hemigrammus lunatusbear anal- and pelvic-fin hooks. Fin hooks were observed in males collected from November through April. Pelvic-fin hooks are anterodorsally arranged almost all over the entire length of all fin rays; usually one pair of hooks per ray segment. The anal fin bears 5-15 tiny hooks, anterodorsally arranged almost over the entire length of the last unbranched ray and first three or four branched fin rays, one pair per ray segment.  Habitat and ecological notes.Habitat preferences are somewhat similar to those presented by  Hemigrammus machadoi. Thus  H. lunatusis commonly found in slow flowing, low-gradient streams and small rivers. In fact, both species are found syntopically at some localities on the rio Guaporé basin. However,  H. lunatushas a substantially broader distribution than  H. machadoiand occurs in habitats where the latter species is unknown, as floodplain lakes associated with white-water rivers in central Amazon. At the río Napobasin in Amazonian Ecuador,  H. lunatuswas found to be an ubiquitous and abundant species found in lagoons and riverine habitats across a piedmont/lowland river stretch ( Galacatos et al., 1996, 2004).   Analysesof dissected specimens, captured from Novemberthrough April, from both rio Madeiraand rio Paraguaibasins, revealed mature females ( INPA 21621, 2 ex.; NUP 2114, 1 ex.; NUP 7531, 2 ex.; NUP 7533, 4 ex.; NUP 8024, 15 ex.), possessing large yellowish oocytes, and mature males ( INPA 21621, 1 ex.; NUP 2113, 1 ex.; NUP 2114, 2 ex.; NUP 7531, 6 ex.; NUP 7533, 4 ex.; NUP 8024, 5 ex.) with lobulated, whitish testicles.   Distribution.  Hemigrammus lunatusis known from the central and western portions of the rio Amazonas basin, in Brazil, Bolivia( e.g., Géry, 1964; Chernoff et al., 2000), Peru( e.g., Géry, 1964, Barthem et al., 2003), Ecuador( e.g., Galacatos et al., 1996), and Colombia( e.g., Mojica et al., 2005), and along rio Paraguaibasin in Braziland Paraguay. The species is also recorded from the upper Corantijn River in Suriname, near the boundary with Brazil( Géry, 1965; Mol, 2012) and from the upper río Orinoco and río Caura in Venezuela( Lasso et al., 2004). The map shows the localities of the material examined in the present study ( Fig. 6). For comments on the identity of the remaining populations, see “Remarks”.   Remarks.  Hemigrammus lunatuswas described by Durbin (in Eigenmann, 1918: 164-165), though the name had previously appeared without a description or diagnosis in Eigenmann (1910: 436)and Ellis (1911: 162). Although paratypesof  Hemigrammus lunatuswere collected both from the rio Paraguai(Jauru and Cáceres) and Amazon basins (Óbidos), no information on the locality of the holotypewas provided at the description, and the type locality is indicated simply to be “Amazon basin” by Durbin (in Eigenmann, 1918: 165). However, the holotypeand the two paratypes(one paratypeis missing), that belong to the same lot (MCZ 20964) actually have not lost their locality data ( Figs. 4and 6). The holotypeand these two paratypeswere collected at the lago Badajós, a large blocked-valley lake tributary of the rio Solimões/ Amazonas near Codajás, Amazonas ( ca. 03º21’S 62º41’W). We therefore restrict the type locality of  Hemigrammus lunatusto “ Brazil, Amazonas, Codajás, lago Badajós ( ca. 03º21’S 62º41’W)”. Although the holotypeis mixed with two paratypesin the same lot, the largest specimen was designated by Durbin (in Eigenmann, 1918) as the “type” of the species. Notwithstanding we have not examined material collected at the type locality, lago Badajós lies about 260 kmin a straight line upstream from the lago Janauacá, a very similar blocked-valley lake from where much material of the species is available in collections (see Material examined). Géry (1964: 9-10)provided a short redescription of the species based on specimens from the Amazon basin in Peruand Boliviaconsidered that the population of  H. lunatusoccurring in the rio Paraguaibasin might be distinct at the subespecific level, since the comparison of the specimens from the Amazon basin with a single specimen from the rio Paraguaibasin in Paraguayshowed the latter to be more elongated, and displaying a narrower infraorbital 3 and a longer maxillary. Géry (1964: 10)even suggested that  Hyphessobrycon maxillarismight be an available name for that purported subspecies. The examination of extensive material from the Central Amazon, rio Madeira, and rio Paraguaibasins during the present study did not reveal any features that might distinguish these different populations, and thus they are herein considered to represent a single species ( Fig. 7). We have examined very few specimens of  H. lunatusfrom western Amazon other than from the rio Madeira basin and for the moment we refrain from discussing their presumable conspecificity with the populations from Central Amazon, rio Madeira, and rio Paraguaibasins. Records of  H. lunatusfrom the upper Corantjin River in Suriname( Géry, 1965; Mol, 2012) and rio Orinoco basin in Venezuela( Lasso et al., 2004) need to be reexamined. For a discussion on the synonym of  Hyphessobrycon maxillaris, see the Discussion.   Material examined. Type specimens:  Hemigrammus lunatus: CAS42679, paratype, 1, 23.8 mmCP;  MCZ20964, holotype, 1, 26.5 mmSL, paratypes, 2, 21.7-23.9 mmSL, Codajás, lago Badajós, ca. 3º21’S, 52º41’W.  Non-type specimens: Brazil: Amazonas State, rio Negrobasin: INPA 15339, 1, 36.8 mmSL, Manaus, igarapé Tarumãzinho, road BR-174, km 28 rio Tarumã-Açu, 02º43’51”S 60º04’46”W.  Rio Solimõesbasin: MZUSP 100860, 1, 23.4 mmSL, Manaus, rio Amazonas, lago Puraquequara, ca. 03º02’S 59º49’W;  INPA 24765, 2, 24.5-26.4 mmSL;  NUP 8023, 8, 23.2-27.0 mm SL, Careiro da Várzea, ilha Marchantaria, ca. 03º14’S 59º56’W;  MZUSP 101287, 1, 24.4 mmSL, Careiro da Várzea, ilha Marchantaria, lago Camaleão, ca. 03º14’S 59º56’W;  MZUSP 100794, 1, 24.5 mmSL, Careiro da Várzea, ilha Marchantaria, ca. 03º14’S 59º56’W;  NUP 8024, 21(16, 25.5-29.9 mmSL);  NUP 9573, 4c&s, 25.8-29.3 mmSL, Manaquiri, lago Murumuru, lago Janauacásystem, 03º25’43”S 60º16’00”W;  MCP37131, 2, 23.0- 23.4 mmSL, Manaquiri, lago Murumuru, lago Janauacásystem, 03º25’43”S 60º16’00”W;  MCP37151, 3, 19.0- 23.2 mmSL, Manaquiri, lago Murumuru, lago Janauacásystem, 03º25’43”S 60º16’00”W;  MCP43956, 19, 22.8- 26.1 mmSL, Manaquiri, lago Murumuru, lago Janauacásystem, 03º25’43”S 60º16’00”W;  MCP37149, 2, 26.6-27.5 mmSL, Manaquiri, paraná de Janauacá, mouth of lago Castanho, 03º24’S 60º16’W;  MZUSP 102965, 1, 23.8 mmSL, Manaquiri, lago do Castanho, São José, lago Janauacásystem, 03º24’S 60º16’W;  MZUSP 102737, 1, 26.0 mm SL, Manaquiri, parana de Janauacá, mouth of lago Castanho, 03º24’S 60º16’W;  MZUSP 102722, 2, 24.0- 26.9 mmSL, Manaquiri, lago Murumuru, lago Janauacásystem, ca. 03º24’S 60º16’W;  MZUSP 102980, 7, 22.2-29.8 mmSL, Manaquiri, channel between lago Murumuruand paraná de Janauacá, 03º24’S 60º16’W;  MZUSP 102730, 16, 23.8-33.1 mmSL, Manaquiri, lago Murumuru, lago Janauacásystem, ca. 03º24’S 60º16’W;  MZUSP 102754, 9, 19.5-31.4 mmSL, Manaquiri, channel between lago Murumuruand paraná de Janauacá, ca. 03º24’S 60º16’W;  MZUSP 100463, 1, 30.1 mmSL, Manaquiri, lago do Castanho, São José, lago Janauacásystem, 03º24’S 60º16’W.  Pará State: ZUEC 7995(103, 19.7-28.0 mm SL), Santarém, lago do Pajaú(or Tamoatá), rio Amazonas, 2º11’29”S, 54º51’28”W.  Rondônia State: INPA 21621, 17, 25.9- 33.2 mmSL;  INPA 22571, 7, 27.5-30.2 mmSL, Guajará-Mirim, rio Pacaás-Novos, 10º52’S 65º16’W;  INPA 39569, 5, 23.3-27.5 mmSL, Guajará-Mirim, middle rio Cautário, tributary of rio Guaporé, 10°59’05”S 62°56’12”W.  Rio Madeirabasin, Mato Grosso State: MCP45041, 36, 20.5-31.1 mmSL, Comodoro, stream tributary of rio Novo( rio Guaporébasin), road BR-174, 14º13’26’’S 59º41’27’’W;  MCP45022, 18, 24.0- 32.3 mmSL, Pontese Lacerda, rio Pindaituba(tributary of rio Guaporé), BR-174, 15º00’41’’S 59º17’18’’W;  MCP45030, 6, 28.6-31.4 mmSL, Pontese Lacerda, stream tributary of rio Guaporé, road BR-174, 14º55’15’’S 59º17’29’’W;  MCP15724, 6, 20.8-26.2 mmSL;  MZUSP 44457, 5, 20.8-22.9 mmSL, Pontese Lacerda, rio Guaporéand flooded areas, 15º12’S 59º21’W;  MCP45036, 24, 24.7-36.8 mmSL, Pontese Lacerda, rio Bugre(tributary of rio Guaporé), BR-174, 14º51’35’’S 59º17’57’’W;  MCP45993, 1, 29.5 mmSL, Pontese Lacerda, stream tributary of rio Pindaituba, road BR-174, 14º59’53’’S 59º17’10’’W;  MCP45994, 23, 21.5- 28.8 mmSL, Nova Lacerda, rio Galera(tributary of rio Guaporé), balneário Galera, 14º28’59’’S 59º35’07’’W;  MZUSP 95364, 33, 21.9-30.5 mmSL, Vila Bela da Santíssima Trindade, rio Guaporé, 15º00’28”S 59º57’22”W. Rio Paraguaibasin: Mato Grosso State:  MCP15726, 24, 23.2-32.5 mmSL, stream at road Barra do Bugres/ Cáceres, 99 kmsouth of Barra do Bugres, tributary of rio Paraguai, ca. 15º45’S 57º20’W;  MCP15731, 7, 27.1-31.2 mmSL;  MZUSP 44338, 7, 27.1-28.5 mmSL, stream at Porto Estrela, road Barra do Bugres/ Cáceres, 35 kmsouth of Barra do Bugres, tributary of rio Paraguai, 15º24’S 57º15’W;  MCP15733, 11, 24.5-31.8 mmSL, Barra dos Bugres, rio Jauquara(tributary of rio dos Pássaros) at Jauquara, ca. 15º10’S 57º05’W;  MZUSP 44358, 24, 25.4-33.0 mm SL, stream crossing the road Barra do Bugres/ Cáceres, about 90 kmfrom Barra do Bugres, tributary of rio Paraguai, ca. 15º43’S 57º22’W;  MZUSP 44481, 3, 27.0- 30.9 mmSL, rio Sangradouro, at road Cáceres/ Cuiabáabout 90 kmeast from Cáceres, ca. 16º14’S 56º37’W;  MZUSP 44473, 1, 31.8 mmSL, Cáceres, ribeirão das Flexas, road Cáceres/ Cuiabá,  69 kmE Cáceres, ca. 16º09’S 57º21’W;  MZUSP 44285, 2, 27.7-30.0 mm SL, Jangada, ribeirão Chiqueirão,  21 kmW Jangada, tributary of rio Jangada, ca. 15º07’S 56º39’W;  MZUSP 90076, 12, 22.2-31.0 mm SL, Cáceres, rio Sepotuba, near its mouth, 15º53’34’’S 57º38’44’’W;  MZUSP 90142, 11, 19.8-28.3 mmSL, Cáceres, mouth of rio Sepotuba, 15º54’53”S 57º39’24”W;  MZUSP 90196, 11, 17.7- 29.4 mmSL, Cáceres, lower rio Sepotuba, 15º47’33”S 57º39’20”W;  MZUSP 90274, 41, 26.1-31.6 mmSL, Cáceres, lower rio Sepotuba, 15º46’07”S 57º38’54”W;  MZUSP 90475, 7, 25.7-32.2 mmSL, Cáceres, lower rio Sepotuba, 15º43’53’’S 57º39’53’’W;  MZUSP 90498, 6, 26.9-30.1 mmSL, Cáceres, rio Sepotuba(middle portion), 15º28’44’’S 57º41’59’’W;  MZUSP 90481, 2, 26.6-32.3 mmSL, Cáceres, rio Sepotuba(middle portion), 15º31’01’’S 57º42’26’’W;  MZUSP 4443, 3, 21.9-22.3 mmSL, Santo Antônio do Leverger, rio Cuiabásystem;  NUP 969, 2, 27.1-28.5 mmSL, Barão de Melgaço, rio Cuiabá, 16º20’02”S 55º57’10”W;  NUP 2112, 1, 35.9 mmSL, Rosário Oeste, córrego Imbaúba, tributary of rio Cuiabá, 14º55’06”S 56º27’02”W;  NUP 2113, 2, 21.2-22.7 mmSL, Rosário Oeste, córrego São Joaquim, tributary of rio Cuiabá, 14º44’58”S 56º07’39”W;  NUP 2114, 3, 28.5-34.2 mmSL;  NUP 7528, 5, 22.7-26.1 mmSL, Barão de Melgaço, córrego Cancela, tributary of rio Cuiabá, 14º42’30”S 56º15’51”W;  NUP 9574, 4c&s, 27.3-32.4 mmSL, Rosário Oeste, córrego Cancela, tributary of rio Cuiabá, 14º42’30”S 56º15’51”W;  NUP 2115, 7, 25.8-28.6 mmSL, Chapada dos Guimarães, rio Quilombo, tributary of Manso Reservoir, 15º06’50”S 55º40’38”W;  NUP 9548, 3c&s, 19.3-27.2 mmSL, Chapada dos Guimarães, rio Quilombo, tributary of Manso Reservoir, 15º06’50”S 55º40’38”W;  NUP 7527, 1, 30.7 mmSL, Chapada dos Guimarães, rio Casca, tributary of Manso Reservoir, 14º57’07”S 55º42’59’ W. Mato Grosso do Sul State:  MZUEL 5414, 5, 23.2-33.2 mmSL, Corumbá, bridge on corixo Sará, Estrada Parque, 19º00’29”S 57º39’08”W;  MZUEL 5415, 1, 25.5 mmSL, Corumbá, Base de Estudos do Pantanal ( BEP), right margin of rio Miranda, 19º34’37”S 57º00’42”W;  MZUEL 5416, 1, 24.8 mmSL, Corumbá, Estrada Parque, 9 kmfrom Base de Estudos do Pantanal( BEP), 19º30’45”S 57º02’27”W;  MZUSP 59467, 10, 25.8-30.8 mmSL, Corguinho, stream at Chacara da Portela, tributary of rio Aquidauana, 19º48’40’’S 54º50’39’’W;  NUP 9881, 1, 28.7 mmSL, Porto Murtinho, riacho Amonguijá, tributary of rio Paraguai, 21º41’16”S 57º52’55”W.  Paraguay. Concepción: MZUSP 54028, 55, 26.2-39.9 mmSL, riacho Blandengue, near mouth with río Apa, 22º14’00”S 57º21’00”W;  MZUSP 54027, 21, 29.9-38.9 mmSL, Arroyo La Paz, below bifurcation of the mouth of Riacho Primavera, 22º24’S 57º43’W;  MZUSP 54354, 3, 32.6-33.3 mmSL, Alto Paraguay, río Paraguay, marginal lagoon, estancia Cerrito, 21º27’00”S 57º55”00”W.  Canindeyú: MNHNP 3246, 14(13, 19.5- 36.8 mmSL), oxbow lake of río Jejuí-Mí, 1 kmfrom headquarters of Reserva Natural del Bosque Mbaracajú, 24º08’27”S 55º38’25”W;  MNHNP 3619, 4, 29.9-36.5 mmSL, río Jejuí-Mí, Reserva Natural Del Bosque Mbaracajú, PuestoJejuí-Mí, 24º08’27”S 55º38’25”W. 3114453404 [1048,1390,622,644] Brazil Jauru -3.35 Amazon 1307 -62.683334 Caceres 5 270 1 3114453396 [1102,1294,737,759] Brazil Descalvados 5 270 1 Mato Grosso 3114453359 INPA, NUP Paraguay rio Madeira Analyses April 7 272 INPA 21621, NUP 2114, NUP 7531, NUP 7533, NUP 8024, NUP 2113 43 3114453403 CAS 42679 [725,771,1323,1344] 9 274 1 paratype 3114453387 MCZ 20964 MCZ Codajas & Badajos -3.35 1307 -52.683334 9 274 2 holotype 3114453370 INPA Manaus & Tarumazinho Brazil -2.7308333 rio Taruma-Acu 21 -60.079445 rio Negro 9 274 INPA 15339, 1 1 Amazonas State 3114453360 MZUSP Manaus & Puraquequara Brazil -3.0333333 rio Amazonas 1307 -59.816666 Rio Solimoes 9 274 MZUSP 100860, 1 1 Amazonas State 3114453318 [129,452,1599,1621] INPA Brazil 9 274 INPA 24765, 2 1 Amazonas State 3114453356 NUP Brazil -3.2333333 ilha Marchantaria 1307 -59.933334 Careiro da Varzea 9 274 NUP 8023, 8, 23.2 1 Amazonas State 3114453329 MZUSP Camaleao Brazil -3.2333333 ilha Marchantaria 1307 -59.933334 Careiro da Varzea 9 274 MZUSP 101287, 1 1 Amazonas State 3114453357 MZUSP Brazil -3.2333333 ilha Marchantaria 1307 -59.933334 Careiro da Varzea 9 274 MZUSP 100794, 1 1 Amazonas State 3114453358 NUP Brazil 9 274 NUP 8024, 21 1 Amazonas State 3114453379 NUP Manaquiri & Murumuru & Janauaca Brazil -3.4286113 21 -60.266666 9 274 NUP 9573, 4 1 Amazonas State 3114453325 MCP 37131 MCP Manaquiri & Murumuru & Janauaca Brazil -3.4286113 21 -60.266666 9 274 2 1 Amazonas State 3114453381 MCP 37151 MCP Manaquiri & Murumuru & Janauaca Brazil -3.4286113 21 -60.266666 9 274 3 1 Amazonas State 3114453355 MCP 43956 MCP Manaquiri & Murumuru & Janauaca Brazil -3.4286113 21 -60.266666 9 274 19 1 Amazonas State 3114453307 MCP 37149 MCP Manaquiri & de Janauaca & Castanho Brazil -3.4 1307 -60.266666 9 274 2 1 Amazonas State 3114453395 MZUSP Manaquiri & Castanho & Sao Jose & Janauaca Brazil -3.4 1307 -60.266666 9 274 MZUSP 102965, 1 1 Amazonas State 3114453394 MZUSP Manaquiri & de Janauaca & Castanho Brazil -3.4 1307 -60.266666 9 274 MZUSP 102737, 1 1 Amazonas State 3114453380 MZUSP Manaquiri & Murumuru & Janauaca Brazil -3.4 1307 -60.266666 9 274 MZUSP 102722, 2 1 Amazonas State 3114453365 MZUSP Manaquiri & Murumuru & de Janauaca Brazil -3.4 1307 -60.266666 9 274 MZUSP 102980, 7 1 Amazonas State 3114453391 MZUSP Manaquiri & Murumuru & Janauaca Brazil -3.4 1307 -60.266666 9 274 MZUSP 102730, 16 1 Amazonas State 3114453342 MZUSP Manaquiri & Murumuru & de Janauaca Brazil -3.4 1307 -60.266666 9 274 MZUSP 102754, 9 1 Amazonas State 3114453407 MZUSP Manaquiri & Castanho & Sao Jose & Janauaca Brazil -3.4 1307 -60.266666 9 274 MZUSP 100463, 1 1 Amazonas State 3114453319 ZUEC Santarem & Pajau & Tamoata Brazil -2.1913888 rio Amazonas 21 -54.857777 9 274 ZUEC 7995 1 Para State 3114453383 INPA Brazil Rondonia State 9 274 INPA 21621, 17 1 Rondonia 3114453386 INPA Guajara-Mirim Brazil -10.866667 rio Pacaas-Novos 1296 -65.26667 9 274 INPA 22571, 7 1 Rondonia 3114453322 INPA Guajara-Mirim Brazil -10.984722 rio Guapore 21 -62.93667 rio Cautario 9 274 INPA 39569, 5 1 Rondonia 3114453385 MCP 45041 MCP Comodoro Brazil Rio Madeira -14.223888 rio Guapore 21 -59.690834 rio Novo 9 274 36 1 Mato Grosso 3114453374 MCP 45022 MCP Pontes & Lacerda Brazil -15.011389 rio Guapore 21 -59.288334 rio Pindaituba 9 274 18 1 Mato Grosso 3114453352 MCP 45030 MCP Pontes & Lacerda Brazil -14.920834 rio Guapore 21 -59.29139 9 274 6 1 Mato Grosso 3114453405 MCP 15724 MCP Brazil 9 274 6 1 Mato Grosso 3114453310 MZUSP Pontes & Lacerda Brazil -15.2 rio Guapore 1286 -59.35 9 274 MZUSP 44457, 5 1 Mato Grosso 3114453332 MCP 45036 MCP Pontes & Lacerda Brazil -14.859722 rio Guapore 21 -59.299168 rio Bugre 9 274 24 1 Mato Grosso 3114453378 MCP 45993 MCP Pontes & Lacerda Brazil -14.998055 rio Pindaituba 21 -59.28611 9 274 1 1 Mato Grosso 3114453408 MCP 45994 MCP Nova Lacerda & Galera Brazil -14.483055 rio Guapore 21 -59.585278 rio Galera 9 274 23 1 Mato Grosso 3114453384 MZUSP Brazil -15.007778 rio Guapore 21 -59.956112 Vila Bela da Santissima Trindade 9 274 MZUSP 95364, 33 1 Mato Grosso 3114453362 [1061,1448,1461,1483] Paraguay Mato Grosso State 9 274 1 Mato Grosso State 3114453301 MCP 15726 MCP Paraguay Barra do Bugres -15.75 Barra do Bugres 1284 -57.333332 Caceres 9 274 24 1 3114453371 MCP 15731 [1029,1361,1564,1586] MCP Paraguay 9 274 7 1 3114453393 MZUSP Porto Estrela Paraguay Barra do Bugres -15.4 Barra do Bugres 1285 -57.25 Caceres 9 274 MZUSP 44338, 7 1 3114453309 MCP 15733 MCP Barra dos Bugres & dos Passaros & Jauquara Paraguay -15.166667 rio Jauquara 1286 -57.083332 9 274 11 1 3114453335 MZUSP Paraguay Barra do Bugres -15.716666 Barra do Bugres 1284 -57.366665 Caceres 9 274 MZUSP 44358, 24, 25.4 1 3114453400 MZUSP Cuiaba Paraguay rio Sangradouro -16.233334 Caceres 1282 -56.616665 Caceres 9 274 MZUSP 44481, 3 1 3114453388 MZUSP das Flexas & Cuiaba Paraguay -16.15 Caceres 1282 -57.35 Caceres 9 274 MZUSP 44473, 1 1 3114453364 MZUSP Chiqueirao Paraguay 11 276 -15.116667 rio Jangada 1286 -56.65 Jangada 9 274 MZUSP 44285, 2, 27.7 1 3114453367 MZUSP Paraguay -15.892777 rio Sepotuba 21 -57.645557 Caceres 11 276 MZUSP 90076, 12, 22.2 1 3114453353 MZUSP Paraguay -15.9147215 rio Sepotuba 21 -57.65667 Caceres 11 276 MZUSP 90142, 11 1 3114453328 MZUSP Paraguay -15.7925005 lower rio Sepotuba 21 -57.655556 Caceres 11 276 MZUSP 90196, 11 1 3114453382 MZUSP Paraguay -15.768611 lower rio Sepotuba 21 -57.648335 Caceres 11 276 MZUSP 90274, 41 1 3114453306 MZUSP Paraguay -15.731388 lower rio Sepotuba 21 -57.664722 Caceres 11 276 MZUSP 90475, 7 1 3114453392 MZUSP Paraguay -15.4788885 rio Sepotuba 21 -57.699722 Caceres 11 276 MZUSP 90498, 6 1 3114453337 MZUSP Paraguay -15.516944 rio Sepotuba 21 -57.707222 Caceres 11 276 MZUSP 90481, 2 1 3114453350 MZUSP Paraguay rio Cuiaba Santo Antonio do Leverger 11 276 MZUSP 4443, 3 1 3114453368 NUP Paraguay -16.333889 rio Cuiaba 21 -55.952778 Barao de Melgaco 11 276 NUP 969, 2 1 3114453398 NUP Rosario Oeste & Imbauba Paraguay -14.918334 rio Cuiaba 21 -56.450558 11 276 NUP 2112, 1 1 3114453330 NUP Rosario Oeste & Sao Joaquim Paraguay -14.749445 rio Cuiaba 21 -56.1275 11 276 NUP 2113, 2 1 3114453354 NUP Paraguay 11 276 NUP 2114, 3 1 3114453320 NUP Cancela Paraguay -14.708333 rio Cuiaba 21 -56.264168 Barao de Melgaco 11 276 NUP 7528, 5 1 3114453340 NUP Rosario Oeste & Cancela Paraguay -14.708333 rio Cuiaba 21 -56.264168 11 276 NUP 9574, 4 1 3114453326 NUP Chapada dos Guimaraes & Manso Reservoir Paraguay -15.11389 rio Quilombo 21 -55.677223 11 276 NUP 2115, 7 1 3114453338 NUP Chapada dos Guimaraes & Manso Reservoir Paraguay -15.11389 rio Quilombo 21 -55.677223 11 276 NUP 9548, 3 1 3114453343 NUP Chapada dos Guimaraes & Manso Reservoir & W Paraguay -14.951944 rio Casca 21 -57.652225 11 276 NUP 7527, 1 1 Sul State 3114453323 [257,1359,183,1172] NUP Corumba & Sara & Estrada Parque Paraguay -14.951944 rio Casca 21 -57.652225 11 276 NUP 7527, 1 1 Sul State 3114453347 BEP Paraguay -19.576946 rio Miranda 21 -57.011665 Corumba 11 276 MZUEL 5415, 1 1 Sul State 3114453331 BEP Paraguay Corumba -19.5125 Base de Estudos do Pantanal 21 -57.040833 Estrada Parque 11 276 MZUEL 5416, 1 1 Sul State 3114453349 MZUSP Corguinho Paraguay -19.81111 rio Aquidauana 21 -54.844166 Chacara da Portela 11 276 MZUSP 59467, 10 1 Sul State 3114453302 NUP Porto Murtinho & Amonguija Paraguay -21.687777 Paraguai 21 -57.881943 11 276 NUP 9881, 1 1 3114453341 MZUSP Blandengue Paraguay -22.233334 rio Apa 21 -57.35 11 276 MZUSP 54028, 55 1 Concepcion 3114453304 MZUSP Riacho Primavera Paraguay -22.4 Arroyo La Paz 1260 -57.716667 11 276 MZUSP 54027, 21 1 Concepcion 3114453375 MZUSP Cerrito Paraguay -21.45 Alto Paraguay 21 -55.640278 11 276 MZUSP 54354, 3 1 Alto Paraguay 3114453308 MNHNP Paraguay -24.140833 Reserva Natural del Bosque Mbaracaju 21 -55.640278 11 276 MNHNP 3246, 14 1 Canindeyu 3114453316 MNHNP Puesto Paraguay -24.140833 Reserva Natural Del Bosque Mbaracaju 21 -55.640278 11 276 MNHNP 3619, 4 1 Canindeyu