Five new species of Tupiperla Froehlich, 1969 (Plecoptera: Gripopterygidae) Duarte, Tácio Novaes, Marcos Carneiro Bispo, Pitágoras Da Conceição Zootaxa 2019 2019-09-19 4671 4 511 526 8NGRJ Duarte & Novaes & Bispo, 2019 Duarte & Novaes & Bispo 2019 [151,354,1426,1453] Insecta Gripopterygidae Tupiperla Animalia Plecoptera 2 513 Arthropoda species pinhoi sp. nov.  ( Figs. 2A–B, Figs. 3A–C)   Material Examined.    Holotype. Male.  Brazil: Santa Catarina: Urubici, Cachoeira do Avencal,  1229 ma.s.l., S28°02.798, W49°37.070,  08.i.2010, net, HF Mendes, LC Pinho& RA Morettocol. ( MZSP).  Measurement.  Holotype, male: head width, 1.0 mm; pronotum width, 0.83 mm; pronotum length, 0.64 mm; forewing length, 7.9 mm; hind wing length, 6.7 mm; antenna length, 8.1 mm; 15 cercomeres.   Description.  Tupiperla pinhoi  sp. nov.is a medium sized species. General color brownish to ochraceous. Head brownish with a lighter area in front of the coronal fork, and laterally to the eyes ( Fig. 2A); ocelli and eyes black; anterior part of frons brown; labrum light brown, darker than clypeus; lateral part of the head, behind the eyes, with a dark brown band. Maxillary and labial palpi ochraceous. Antenna light brown. Pronotum squarish, light brown, narrower than the head and with some little setae covering it ( Fig. 2A). Legs light brown, covered with thin bristles; femur with disto-ventral spine; tibia with two distal spurs; tarsi with tarsomere 1 medium, tarsomere 2 short, and tarsomere 3 long. Wings membranous, light brown; forewing with a conspicuous darker pattern bordering veins and crossveins; pterostigmatic crossvein absent, RA unforked, RP forked and CuA long-forked; hind wing with M3+4, near its separation from M1+2, fused with CuA in part of its length, CuA short forked, 6th anal vein fused with hind margin of wing ( Fig. 2B).   TABLE 1.  Tupiperlaspecies, records and described life stages.    Species Author, year Records  Known life stages    Tupiperla: ARG (MIS), BRA (BA, ES, GO, MG, PE, RJ, RS, SC, SP), PRY (PRI)  M  F  N   1   T. amandae  Bispo & Lecci, 2011  BRA (SP) ● ● -   2   T. amorimi  Froehlich, 2016  BRA (MG) ● ● -   3   T. barbosai Avelino-Capistrano &  BRA (RJ) ● - -   4   T. bispoi Nessimian, 2013 Duarte, Lecci & Calor, 2014b  BRA (BA) ● - -   5   T. eleonorae ( Froehlich, 1994)  BRA (SP) ● ● -   6   T. flinti  Froehlich, 2002  ARG (MIS), PRY (PRI) ● ● -   7   T. froehlichi  Bispo & Lecci, 2011  BRA (SP) ● - -   8  T. gracilis ( Burmeister, 1839)  BRA (RJ, SP, MG, ES) ● ● ?   9   T. guariru  Duarte, Bispo & Calor, 2014a  BRA (BA) ● ● -   10   T. illiesi  Froehlich, 1998  BRA (SP) ● ● -   11   T. jumirim  Bispo & Froehlich, 2007  BRA (GO) ● ● -   12   T. misionera  Froehlich, 2002  ARG (MIS), BRA (RS) ● - -   13   T. modesta  Froehlich, 1998  BRA (SP) ● ● -   14   T. oliveirai  Froehlich, 1998  BRA (GO) ● ● -   15   T. pessacqi  sp. nov. Here  BRA (SC) ● ● -   16   T. pinhoi  sp. nov. Here  BRA (SC) ● - -   17  T. reichardti  Froehlich, 1998  BRA (MG, SP, RJ) ● ● -   18   T. robusta  Froehlich, 1998  BRA (RJ, SP, ES, MG) ● ● -   19   T. sepeensis  Novaes & Bispo, 2016  BRA (RS) ● - -   20   T. serrulata  sp. nov. Here  BRA (SC) ● ● -   21   T. sulina  Froehlich, 1998  BRA (SC) ● ● -   22  T. tessellata ( Brauer, 1868)  BRA (RJ, MG, SP, BA, PE, ● ● -   23   T. ubuntu  sp. nov. Here  ES, RS) BRA (SC) ● ● -   24   T. umbya  Froehlich, 1998  BRA (SP) ● ● -   25   T. zwicki  sp. nov. Here  BRA (SC) ● - -  ARG, Argentina (MIS, Misiones Province); BRA, Brazil (BA, Bahia State; ES, Espírito Santo State; GO, Goiás State; MG, Minas Gerais State; PR, Paraná State; PE, Pernambuco State; RJ, Rio de Janeiro State; RS, Rio Grande do Sul State; SC, Santa Catarina State; SP, São Paulo State); PRY, Paraguay (PRI, Paraguarí Department);?, when Froehlich (1969)described nymph of  T. gracilis, the genus was considered monotypic; however, according to Froehlich (1998), it is likely that the nymph described in 1969 belongs to another species of the genus.  Male. Abdomen brownish to ochraceous with slightly lighter band on abdominal terga 1–9. T10 light brown to ochraceous. T10 extension, in dorsal view, large, distally darker, ending in two separated and out curved teeth ( Fig. 3A); in lateral view, straight dorsally and with a clear latero-ventral band ( Fig. 3C). Paraprocts, in ventral view, thin, medially curved with thin bristles internally and conspicuous minute teeth on dorsal (outer) margin ( Fig. 3B); in lateral view, with constant width over most of its length, dorsal margin darker and medially covered with minute teeth, distal region with an accentuated hook-shaped curvature ( Fig. 3C). Subgenital plate ochraceous, ovoid-shaped and with apex prolonged between the paraprocts ( Fig. 3B). Epiproct absent.  Female and nymph. Unknown.   Remarks.  Tupiperla pinhoican be distinguished from the congeners by the shape of the paraprocts and the T10 extension in males. The median dorsal margin of the paraprocts in  T. pinhoiare covered by minute teeth, and the distal region has a distinct hook-shaped curvature. Only  T. illiesi,  T. reichardti Froehlich, 1998, and  T. serrulata  sp. nov.have minute teeth on the dorsal margin of the paraprocts. However, in  T. illiesieach paraproct has a small and thin latero-distal spine, whereas in  T. reichardti, each paraproct has a large latero-medial spine and in  T. serrulata  sp. nov., the paraprocts lack a lateral spine.   FIGURE 2.  Tupiperlaadult males. Head, pronotum and wings of  T. pinhoi  sp. nov.(A–B),  T. serrulata  sp. nov.(C–D),  T. pessacqi  sp. nov.(E–F),  T. ubuntu  sp. nov.(G–H), and  T. zwicki  sp. nov.(I–J). Scale bar: 0,5 mm. (Abbreviations: AA1, first anterior Analis; AA2, second anterior Analis; Ant, Antenna; CuA, anterior cubitus; CuP, posterior cubitus; Ey, Eyes; Hd, Head; M, Media; Oc, Ocelli; PC, Pterostigmatic cell; Pnt, Pronotum; RA, anterior Radius; RP, posterior Radius; Sc, Subcosta)   FIGURE 3.  Tupiperla pinhoi  sp. nov.Holotype adult male, terminalia in dorsal (A), ventral (B), and lateral views (C). Scale bar: 0,5 mm. (Abbreviations: Cc, Cerci; Pp, Paraprocts; Sp, Subgenital plate; T9, Tergum 9; T10, Tergum 10; T10e, Tergum 10 extension)   Tupiperla pinhoiwas collected in 2010 at the Avencal Waterfall, at 1229 ma.s.l. in the municipality of Urubici, Santa Catarina, Brazil. The waterfall is 100 mhigh and it formed by the Funil River. The common vegetation in the area is the “avenca” ( Pteridaceae:  Adiantumsp.).   Etymology.The specific name  pinhoihonors Prof. Dr. Luiz Carlos de Pinho for his contribution to aquatic entomology, especially related to Brazilian chironomids. 2416712822 2010-01-08 MZSP HF Mendes & LC Pinho & RA Moretto Brazil 1229 -28.046633 Cachoeira do Avencal 1 -49.617832 Urubici 2 513 1 Santa Catarina holotype