Ecclisister bickhardti costaericae Reichensperger 1935: 204
Ecclisister costaericae
E. burchellii
E. burchellii
E. burchellii
E. burchellii
E. burchellii
E. burchellii
E. burchellii
E. burchellii
E. burchellii
E. burchellii
E. burchellii
Ecclisister species. E
E. costaericae
Ecclisister bickhardti costaericae Reichensperger
Mazur 1984: 304
1997: 159
2011: 116
Helava et al. 1985: 324
Taxonomic Review and Natural History Notes of the Army Ant-Associated Beetle Genus Ecclisister Reichensperger (Coleoptera: Histeridae: Haeteriinae)
Tishechkin, Alexey K.
Kronauer, Daniel J. C.
Beeren, Christoph Von
The Coleopterists Bulletin
2017
2017-06-27
71
2
279
288
9LBRX
Reichensperger, 1935
Reichensperger
1935
Insecta
Histeridae
Ecclisister
Animalia
Coleoptera
3
281
Arthropoda
species
costaericae
stat. nov.
Ecclisister bickhardti costaericae Reichensperger 1935: 204. Table 1.Information for barcoded Ecclisister costaericaepopulations. acc. no. = accession number; AKT = Alexey K. Tishechkin; Coll. = collection; CvB = Christoph von Beeren; Dep. = depository; DK = Daniel Kronauer; GB = Griffin Burke; ID = identification number; Lat. = Latitude; Long. = Longitude; LSAM = Louisiana State Arthropod Museum; SP = Sebastian Pohl. GenBank Voucher Sample ID acc. no. type Dep. Host species Collectors Collec. Date Country Region Exact Site Lat. Lon. cvb266hist002 KX420353 private CvB E. burchellii CvB, DK, 12-Mar-2013 Costa Heredia: LSBS 10.423 -84.022 collection foreli SP, GB Rica Canton Sarapiqui cvb266hist003 KX420352 private CvB E. burchellii CvB, DK, 12-Mar-2013 Costa Heredia: LSBS 10.423 -84.022 collection foreli SP, GB Rica Canton Sarapiqui cvb266hist004 KX420351 private CvB E. burchellii CvB, DK, 12-Mar-2013 Costa Heredia: LSBS 10.423 -84.022 collection foreli SP, GB Rica Canton Sarapiqui cvb285hist001 KX420350 private CvB E. burchellii CvB, DK, 13-Mar-2013 Costa Heredia: LSBS 10.423 -84.022 collection foreli SP, GB Rica Canton Sarapiqui cvb571hist005 KX420348* museum LSAM E. burchellii CvB, DK, 08-Apr-2013 Costa Heredia: LSBS 10.423 -84.022 specimen foreli SP, GB Rica Canton Sarapiqui cvb572hist001 KX420349 museum LSAM E. burchellii CvB, DK, 08-Apr-2013 Costa Heredia: LSBS 10.423 -84.022 specimen foreli SP, GB Rica Canton Sarapiqui cvb572hist003 KX420354 museum LSAM E. burchellii CvB, DK, 08-Apr-2013 Costa Heredia: LSBS 10.423 -84.022 specimen foreli SP, GB Rica Canton Sarapiqui Ecc002 KX420355 museum LSAM E. burchellii AKT 01-Jul-2008 Ecuador Orellana Tiputini -0.638 -76.150 specimen Biodiversity Station Ecc005 KX420347 museum LSAM E. burchellii AKT 28-Mar-2009 Peru Jun´ın Los Olivos -11.050 -74.259 specimen Ecc007 KX420357 museum LSAM E. burchellii AKT 28-Mar-2009 Peru Jun´ın Los Olivos -11.050 -74.259 specimen Ecc008 KX420356 museum LSAM E. burchellii AKT 28-Mar-2009 Peru Jun´ın Los Olivos -11.050 -74.259 paratype * Record taken from von Beeren and Tishechkin (2017). Fig. 1. Ecclisisterspecies. E . bickhardtilectotype: a) dorsal view, b) lateral view, c) ventral view, d) frontal head, e) aedeagus, dorsal view, f) aedeagus, lateral view; E. costaericaelectotype: g) dorsal view, h) lateral view, i) ventral view, j) frontal head, k) aedeagus, dorsal view, l) aedeagus, lateral view. Scale bars: a–c, g–i = 1 mm; d and j = 0.2 mm. Dorsal and lateral views of aedeagi are at the same scale. Ecclisister bickhardti costaericaeReichensperger: Mazur 1984: 304, 1997: 159, 2011: 116; Helava et al.1985: 324 Zoobank Identifier. urn:lsid:zoobank.org:act: 920C67FF-E5B2-47A9-B2C2-31EBCCCDDEA8
Type Material Examined. Lectotype(present designation; Fig. 1g–l). A male mounted on a point and labeled “ Costa Rica F. Nevermann 25.11.1934/ Eciton burchelli/ Type! Reichensperger / Ecclisister bickhardti costaericae/ LECTOTYPE Ecclisister bickhardti costaericaeReich. A. K. Tishechkin and C. von Beerendes. 2015” ( FIMAK). Paralectotype.A specimen of undetermined sex with the same label data as lectotype, but “ Paratype! Reichensperger” instead of “ Type! Reichensperger” and our “ Paralectotype” instead of “ Lectotype” labels and an extra label “ Hamburg Farm Reventazon Ebene Limon” ( FIMAK). Additional Material Examined. BRAZIL(new country record): Pará: 4 specimens: Altamira – Maraba, km 18, 3°09ʹS 52°03ʹW, 14.iv.1986, in an E. burchelliibivouac, N. Dégallier(ND); 1 specimen: Belém, Utinga( IPEAN), 1°27ʹS 48°26ʹW, 18.viii.1984, in a nest of E. burchelliiinside a hollow tree stump, N. Dégallier (ND). COSTA RICA: Heredia: 2 specimens: La Selva Biological Station, 10.4305°N 84.0064°W, 8.iv.2013, with E. burchellii, C. von Beeren, G. Burke, D. Kronauer, S. Pohl (LSAM); 1 specimen: Puerto Viejo, 10°26ʹN 83°59ʹW, 2.viii.1965, with E. burchellii, C. W. Rettenmeyer (UCS). Limon: 29 specimens: Hamburg Farm, 10°13ʹN 83°29ʹW, multiple dates between 10.xi.1927and 1.i.1938and undated, all with E. burchellii, F. Nevermann (FIMAK, FMNH, USNM, ZMHUB). ECUADOR(new country record): Orellana: 1 specimen: Tiputini Biodiversity Station, 0.6376°S 76.1499°W, 31.vii.2008, with E. burchellii, A. K. Tishechkin (LSAM). GUYANA(new country record): Cuyuni-Mazaruni: 1 specimen: Kartabo, 6°23ʹN 58°42ʹW, 15.iv.1919(ND). PANAMA: Colón: 3 specimens: San Lorenzo Forest, Smithsonian Research Institutioncanopy crane site, 9°17ʹN 79°58ʹW, 3.x. 2003and 21.x. 2003, with E. burchellii, A. K. Tishechkin (LSAM). Panamá: 1 specimen: Barro Colorado Island, 9°11ʹN 79°51ʹW, with E. burchellii, O. Park (FMNH); 6 specimens: same locality and host data, multiple dates between 4.iii.1955and 23.iii.1966, C. W. Rettenmeyer( FMNH, UCS); 4 specimens: same locality and host data, multiple dates between 26.iii.1967and 1.v.1967, R. Akre, R. Torgerson( WSU). PERU(new country record): Jun´ın: 5 specimens: 11 kmNW Puerto Ocopa, Los Olivos, 11°3.00ʹS 74°15.52ʹW, 1,220 m, 28. iii.2009, with E. burchellii, riding beneath heads of major workers in emigration column, A. K. Tishechkin( LSAM). Loreto: 1 specimen: R´ıo Sucusari, 2 kmupstream from R´ıo Napo, 3°15ʹS 73°14ʹW, 27.viii.1992, with E. burchellii, P. E. Skelley (PK).
Host Specificity and Phoretic Transport in Ecclisister.We found nine E. costaericaespecimens during Ecitoncolony emigrations at LSBS. They were collected from two of 12 E. burchellii forelicolonies, while we never found any E. costaericaespecimens associated with other host species. However, actual prevalence of E. costaericaein E. burchellii forelicolonies might be higher, because the discovery rate during army ant colony emigrations is expected to be low. Ecclisister costaericaehas been observed to be phoretic on the underside of the heads of major worker ants (AKT, personal observation), which makes it difficult for the collector to spot them. In an emigration of E. burchellii cupiensSantschimonitored in March 2009in the Jun´ın Region of Peru, AKT noticed a lump beneath the head of a passing major worker ant, which turned out to be an attached haeteriine beetle upon inspection. Special attention to the passing major worker ants allowed the collection of multiple specimens of the genera ClientisterReichensperger, Ecclisister, and SternocoelopsisReichenspergeron approximately every 40 thmajor worker ant (see also von Beeren and Tishechkin 2017). Further evidence for phoretic transport of Ecclisisterbeetles on major army ant comes from the collection of specific army ant castes. Five of the nine specimenscollected at LSBS were found in collection vials into which only major workers had been collected, while no Ecclisisterspecimens were found in collection vials of minor and intermediate workers. The remaining four specimenswere found in collection vials with representatives of all worker castes. No data are available about host-following mechanisms in E. bickhardti. Molecular Analysis. COIbarcodes were successfully obtained for four South American E. costaericaespecimens and seven Central American E. costaericaespecimens ( Figure 2A; for detailed collection records as well as GenBank accession numbers, see Table 1). They comprised two and three different COIhaplotypes, respectively. A RAxML tree of COIsequences revealed two distinct genetic clades reflecting the origin of specimens ( Fig. 2B). P-distances within the geographic areas were lower than 1% (range 0.0–0.5%), while they exceeded 2% (range 2.3–2.4%) in pairwise comparisons between specimens from the two geographic areas. P-distances describe the percentage of base-pair differences in two homologous DNA sequences.
1934-11-25
FIMAK
F. Nevermann
Costa Rica
Costa Rica
5
283
2
lectotype
1934-11-25
FIMAK
F. Nevermann
Costa Rica
Reichensperger
Reichensperger
Type
5
283
4
Limon
paralectotype
1986-04-14
IPEAN
N. Degallier & Belem & Utinga
Brazil
-3.15
Altamira - Maraba
1307
-52.05
5
283
5
Para
1965-08-02
2013-04-08
1965-08-02
Costa Rica
10.433333
Puerto Viejo
1298
-83.98333
La Selva Biological Station
5
283
3
Heredia
10. xi. 1927 and 1. i. 1938
Costa Rica
6
284
10.216666
Hamburg Farm
1298
-83.48333
5
283
29
Limon
2008-07-31
Ecuador
-0.6376
Tiputini Biodiversity Station
7
-76.1499
6
284
1
Orellana
1919-04-15
Guyana
6.383333
Kartabo
1304
-58.7
6
284
1
Cuyuni-Mazaruni
3. x. 2003 and 21. x. 2003
Panama
9.283334
Smithsonian Research Institution
1300
-79.96667
San Lorenzo Forest
6
284
3
Colon
4. iii. 1955 and 23. iii. 1966
FMNH, UCS
C. W. Rettenmeyer
Panama
9.183333
Barro Colorado Island
1300
-79.85
San Lorenzo Forest
6
284
7
Panama
26. iii. 1967 and 1. v. 1967
R, WSU
Akre & Torgerson
Panama
9.183333
Barro Colorado Island
1300
-79.85
San Lorenzo Forest
6
284
4
Panama
2009-03-28
LSAM
A. K. Tishechkin
Peru
1220
-11.05
Los Olivos
13
-74.25867
Jun'in
6
284
5
1992-08-27
Peru
-3.25
Napo
1307
-73.23333
Sucusari
6
284
1
Loreto