Lerneca inalata beripocone subsp. nov. (Orthoptera: Phalangopsidae; Luzarinae): a new taxon for the northern Pantanal of Brazil Lima, Raysa Martins Martins, Luciano De Pinho Pereira, Marcelo Ribeiro Ganchev, Todor D. Jahn, Olaf Lhano, Marcos Gonçalves Marques, Marinêz Isaac Schuchmann, Karl-L Zootaxa 2016 4175 4 366 376 [151,483,580,606] Insecta Haglotettigoniidae Lerneca Animalia Orthoptera 3 369 Arthropoda subSpecies inalata beripocone  http://lsid.speciesfile.org/urn:lsid: Orthoptera.speciesfile.org:TaxonName:492155     Typelocality. Brazil, Mato GrossoState, Poconémunicipality, Fazenda Pouso Alegre: -16.50824°, -56.75050°.  Type material.HOLOTYPE (male): CEMT 52041;  BRAZIL, MT, Poconé, Fazenda Pouso Alegre, Pantanal( FPAP), -16,50824°, -56,75050°,  29.x.2013, active collection, Lima, R.M.coll.,  Lerneca inalata beripocone Lima, Martins& Lhanodet.. ALLOTYPE(female):  CEMT52042;  BRAZIL, MT, Poconé, Fazenda Pouso Alegre, Pantanal ( FPAP), -16,50893°, -56,75367°,  25.ix.2013, active collection, Lima, R.M.coll.,  Lerneca inalata beripocone Lima, Martins & Lhano det..    Othermaterial examined. PARATYPES: 6 females  CEMT52043, 52044, 52045, 52046, 52047, 52048;  BRAZIL, MT, Poconé, Fazenda Pouso Alegre, Pantanal, -16,50303°, -56,74533°,  19–29.x.2013, active collection, R.M. Limacoll.; 7 males: 1 male, CEMT 52049;  BRAZIL, MT, Poconé, Fazenda Pouso Alegre, Pantanal, - 16.50384º, -56.73959º,  19.x.2013, active collection, R.M. Limacoll.,  Lerneca inalata beripocone Lima, R.M., Pereira, M.R., Martins, L.P., Lhano, M.G.det.; 1 male,  CEMT52050;  BRAZIL, MT, Poconé, Fazenda Pouso Alegre, Pantanal, -16.50303°, -56.74533°,  01.xi.2013, active collection, Lima, R.M.coll.,  Lerneca inalata beripocone Lima, R.M. Pereira, M.R., Martins, L.P., Lhano, M.G.det.; 1 male,  CEMT52051;  BRAZIL, MT, Poconé, Fazenda Pouso Alegre, Pantanal, -16.50252°, -56.74226°,  07.x.2013, active collection, R.M. Limacoll.,  Lerneca inalata beripocone Lima, R.M., Pereira, M.R., Martins, L.P., Lhano, M.G.det.; 1 male,  CEMT52052;  BRAZIL, MT, Poconé, Fazenda Pouso Alegre, Pantanal, -16.50824°, -56.75050°,  15.x.2013, active collection, R.M. Limacoll.,  Lerneca inalata beripocone Lima, R.M., Pereira, M.R., Martins, L.P., Lhano, M.G.det.; 1 male,  CEMT52053;  BRAZIL, MT, Poconé, Fazenda Pouso Alegre, Pantanal,  23.x.2013, active collection, Lima, R.M.coll.,  Lerneca inalata beripocone Lima, R.M., Pereira, M.R., Martins, L.P., Lhano, M.G.det.; 1 male,  CEMT52054;  BRAZIL, MT, Poconé, Fazenda Pouso Alegre, Pantanal, -16.50824°, -56.75050°,  15.x.2013, active collection, Lima, R.M.coll.,  Lerneca inalata beripocone Lima, R.M., Pereira, M.R., Martins, L.P., Lhano, M.G.det.; 1 male,  CEMT52055;  BRAZIL, MT, Poconé, Fazenda Pouso Alegre, Pantanal, -16.50893°, -56.75367°,  25.ix.2013, active collection, Lima, R.M.coll.,  Lerneca inalata beripocone Lima, R.M., Pereira, M.R., Martins, L.P., Lhano, M.G.det.; 1 male,  CEMT52056;  BRAZIL, MT, Advanced Pantanal Research Base( BAPP) of the Federal Universityof Mato Grosso, -16.500947°, -56.413267°,  28.vii.2013, active collection, R.M. Lima& L.P. Martinscoll.,  Lerneca inalata beripocone Lima, R.M., Pereira, M.R., Martins, L.P., Lhano, M.G.det..   Etymology.In honor of the indigenous tribe ‘Beri-Poconé’ (Bororo ethnic group) which inhabited the region where the subspecies is present and from which originated the name of the municipality of Poconé, Mato Grossostate.   Diagnosis.This subspecies is distinguished by the combination of the following characters: (i) male genitalia with six spines on the ventral region of sclerite B, with the first spine on the anterior portion having a subtle bifurcation ( Fig. 3A); (ii) sclerite C with slightly concave curvature in the direction of the dorsal region of the genitalia ( Fig. 3C); (iii) tegmina: ratio between speculum length and apical area length c.2.9, and radio between width and apical area length c. 3.16 ( Fig. 4A, B).   Description. Head:Dark brown with 4 light brown longitudinal stripes, short bristles over full extent and long black bristles on the frons ( Fig. 2D). Antenna dark brown with scape, pedicel and antennomere whitened in the proximal region. Frons, gena, clypeus, labrum, and mandibles dark brown. White maxillary palpus, with 3 rounded segments and bristles over its entire length ( Fig. 2F). First segment darker on base, and last segment wider and flatter than the others. Labial palpus smaller and darker than maxillary palpus. Thorax and abdomen:Dorsal disc of pronotum with small dark spots, pubescent, with larger and darker bristles on the anterior and posterior margins. Lateral lobe of pronotum dark brown. Metanotum with two drop-shaped stains. The ventral region of the thorax and abdomen light brown, with longitudinal rounded spots that extend all over the abdomen. Prothorax tergites and abdomen dark brown. Metanotal glands like a pair of small dilatations. Tibia and tarsus I, II, and III and femur I and II dark brown, marked with light spots. Tibia I tympanum present on both sides, oval and subequal in size. Femur III darker in the distal half, with dark brown spots, with the inner surface brighter than the outer ( Fig. 2C). Trapezoidal supranal plate light brown, with apex rounded and narrower than the base ( Fig. 2G). Subgenital plate light brown with dark dots, apex with invagination in the middle portion with sclerite B spines ( Fig. 2H). Cerci light brown. Observations of Paratypes.  Genitalia.Sclerite B with rounded apex and 6 ventral spines, the anterior 4 being more developed than the posterior 2 ( Fig. 3A, C). The first spine in the basal portion of sclerite B with slight bifurcation ( Fig. 3A). Sclerite C dorsally projected, concave, with denticles all over the dorsal surface ( Fig. 3A). Mid-region of pseudepiphallus strongly sclerotized, with basal area larger than the apex ( Figs. 3A, B). Sclerotized proximal pseudepiphallus invagination bent and projected towards the ectophallic apodeme ( Fig. 3C). Pseudepiphallic paramere membranous in more than 50% of its area slightly longer and curved to the medio-anterior portion of the dorsal face ( Fig. 3C).   FIGURE 4.  Lerneca inalata beripocone  subsp. nov.(Female paratype). Tegmina, upper left, dorsal view: A. Left side; B. Right side. Subgenital plate and ovipositor, upper right: C. Ventral view; D. Ovipositor apex, dorsal view; E. Ovipositor apex, side view. Lower row, copulatory papilla: F. Ventral view; G. Side view; H. Dorsal view.  Left tegmen.Brown with light yellow stains, lighter colored veins, speculum length 2.92 ± 0.13 mm(n=16), speculum width 3.19 ± 0.14 mm(n=16); length of apical area 1.01 ± 0.1 mm(n=16). Speculum length-apical area length ratio is 2.95 (2.92 x 1.01 = 2.95) and speculum width-apical area length ratio is 3.22 (3.19 x 1.01 = 3.22). Stridulatory row with 145 ± 11.54 teeth (129–168, n=15). Right tegmen smaller and lighter than left ( Fig. 2).  Calling songs.( Fig. 5, n=3, BIINORPHLEINBE-45IPBC, 28ºC, 7:24 pm, 18.09.2013; BIINORPHLEINBE- 22IPBC, 23ºC, 06:00 pm, 28.07.2013; BIINORPHLEINBE-90IPBC, 28ºC, 06:30 pm, 15.10.2013). Chirps with 29 to 40 pulses, frequency band between 4.0 and 5.7 kHz; dominant frequency 4.5 kHz, each chirp preceded by a single pulse with lower frequency and duration. Pulse frequencies and durations increase gradually over the whole chirp, chirp duration of 253 ± 29.38 ms (n=15), interval between chirps of 707 ± 216.62 ms (n=15), pulse duration of 4.35 ± 0.63 ms, inter-pulse interval of 3.91 ± 0.41 ms (n=157), pulse period of 8.35 ± 0.78 ms (n=157), pulses with 17.77 ± 5.28 sound cycles (n=9), calling song activities during twilight and nocturnal periods, and during the day (one observation at 09:00 am, 27ºC, BIINORPHLEINBE-89IPBC).  Female.Similar to male. Larger in size ( Table 1). Differs from male by tegmina with parallel longitudinal veins. Very small subgenital plate, truncated apex, concave margin, 2 drop-shaped dark brown spots ( Fig. 4A). Ovipositor dorso-ventrally flattened, valve apex slightly acuminated, smooth edge ( Fig. 4C–E).   TABLE 1.Body measurements of  L. inalatamales and females, measurements (mm), with max., min., mean, and standard deviation values. Abbreviations: BL = body length; PL = pronotum length; PW = pronotum width; IOD = interocular distance; RTL = right tegmen length; RTW = right tegmen width; PFL = posterior femur length; PTL = posterior tibia length; OL = ovipositor length.    BL PL PW IOD RTL RTW PLF PTL OL    L. i. beripocone  subsp. nov.Max. Males n=16 Min. 11 9.7 2.2 1.6 2.9 2.1 1.3 1 9 7.4 5.7 4.1 8.3 7.7 7.7 7.4 - -  Mean 10 1.8 2.5 1.2 8.5 5.3 7.9 7.45 -  SD 0.54 0.19 0.23 0.15 0.5 0.44 0.2 0.2 -  Females Max. n=4 Min. 12 11 2.2 1.9 3 2.6 1.6 1.3 7.6 6.7 4.3 3.8 9 8.5 8.4 7.8 8.5 7.8  Mean 11.9 2 2.8 1.5 7 4.1 7.65 8.15 8.3  SD 0.47 0.12 0.18 0.12 0.45 0.25 0.21 0.27 0.29   L. i. mexicanaGorochov, Max. 2007 Min. *Male 1 0 9.5 1.9 1.7 - - - - 8.5 8 - - 8.6 8.4 - - - -  *Female Max. 11 2.3 - - 7.5 - 9.5 - 7.3  Min. 1 0 2.1 - - 7.2 - 9 - 7   L. i. amboroGorochov, Max. 2014 Min. *Male 9.5 8 1.7 1.5 - - - - 8 7 - - 8 7 - - - -  *Female Mean 10 1.9 - - 7 - 8 - 6.8   L. i. pantanalGorochov, Max. 2014 Min. *Male 1 0 8 1.8 1.6 - - - - 8.5 7 - - 8.5 7.5 - - - -  *Female Max. 11 2.2 - - 8 - 8 - 7  Min. 9 1.8 - - 7 - 7.5 - - *Numbers of studied individuals not provided.  Female genitalia.Copulatory papilla elongated, tapered apex with transverse folds ( Figs. 4F–H). Basal portion covered by membranes that separate the papilla from the elongated channel that extends to the small rounded spermatheca.  Occurrence.Known only from the municipality of Poconé, Mato Grosso, Brazil ( Fig. 1). Individuals of this subspecies were frequently observed on leaf litter inside the deciduous and semi-deciduous forests, or near the border areas between forest and pasture. 1323865326 [151,1403,754,779] Brazil Fazenda Pouso Alegre Pocone 3 369 1 Mato Grosso holotype 1323865320 2013-10-29 FPAP, CEMT Lima, R. M. Brazil Pocone Pantanal Fazenda Pouso Alegre 3 369 CEMT 52042 1 Lima allotype 1323865321 2013-09-25 FPAP Lima, R. M. Brazil Lima 3 369 1 Lima allotype 1323865336 [199,1432,970,995] CEMT Other 3 369 CEMT 52043, 52044 6 6 paratype 1323865337 2013-10-19 2013-10-29 2013-10-19 R. M. Lima Brazil Pocone Pantanal Fazenda Pouso Alegre 3 369 7 7 paratype 1323865335 2013-10-19 CEMT R. M. Lima Brazil Pocone Lima, R. M. & Pereira, M. R. & Martins, L. P. & Lhano, M. G. Pantanal Fazenda Pouso Alegre 3 369 CEMT 52050 1 1 paratype 1323865323 2013-11-01 CEMT Lima, R. M. Brazil Pocone Pereira, M. R. & Martins, L. P. & Lhano, M. G. Pantanal Fazenda Pouso Alegre 3 369 CEMT 52051 1 1 Lima paratype 1323865334 2013-10-07 CEMT R. M. Lima Brazil Pocone Lima, R. M. & Pereira, M. R. & Martins, L. P. & Lhano, M. G. Pantanal Fazenda Pouso Alegre 3 369 CEMT 52052 1 1 paratype 1323865322 2013-10-15 CEMT R. M. Lima Brazil Pocone Lima, R. M. & Pereira, M. R. & Martins, L. P. & Lhano, M. G. Pantanal Fazenda Pouso Alegre 3 369 CEMT 52053 1 1 paratype 1323865333 2013-10-23 CEMT Lima, R. M. Brazil Pocone Lima, R. M. & Pereira, M. R. & Martins, L. P. & Lhano, M. G. Pantanal Fazenda Pouso Alegre 3 369 CEMT 52054 1 1 paratype 1323865332 2013-10-15 CEMT Lima, R. M. Brazil Pocone Lima, R. M. & Pereira, M. R. & Martins, L. P. & Lhano, M. G. Pantanal Fazenda Pouso Alegre 3 369 CEMT 52055 1 1 paratype 1323865330 2013-09-25 CEMT Lima, R. M. Brazil Pocone Lima, R. M. & Pereira, M. R. & Martins, L. P. & Lhano, M. G. Pantanal Fazenda Pouso Alegre 3 369 CEMT 52056 1 1 paratype 1323865331 2013-07-28 BAPP R. M. Lima & L. P. Martins & Lima, R. M. & Pereira, M. R. & Martins, L. P. & Lhano, M. G. Brazil Federal University Advanced Pantanal Research Base 3 369 1 Mato Grosso paratype