Andiorrhinus (Turedrilus) miricuri Hernández-García & James, 2018

Hernández-García, Luis Manuel, Bartz, Marie Luise, Guerrero, Jesús Enrique Burgos-, Sousa, Sandriel Costa, Rousseau, Guillaume Xavier & James, Samuel Wooster, 2018, Additions to Andiorrhinus (Turedrilus) (Rhinodrilidae, Clitellata) from Eastern Amazonia, Zootaxa 4496 (1), pp. 481-491 : 483-486

Andiorrhinus (Turedrilus) miricuri Hernández-García & James , sp. n.

( Figure 2 A–F )

Holotype. MPEG.ANL 0 0 0 316 adult, incomplete (posterior end missing). Logged primary forest, Centro novo do Maranhão, Maranhão, Brazil, 3°41'10.76''S 46°45'6.91''W, 158 masl. 28 March 2015, Hernández-García, L.M. & Rousseau G.X. colls.

Paratypes. MPEG.ANL 0 0 0 317, one adult, MPEG.ANL 0 0 0 318, one adult, MPEG.ANL 0 0 0 319, one adult. Same locality as holotype. MPEG.ANL 0 0 0 320 one adult, amputee. Secondary forest , Gurupi settlement, Canãa Village, Itinga do Maranhão, Maranhão, Brazil, 4°2'S 46°54'W, 228 masl. 27 June 2014, Muñoz J.A. & Rousseau G.X. colls.

Other material. MPEG.ANL 0 0 0 367, one adult, complete. Secondary forest 15 years old, Centro novo do Maranhão, PPBio Gurupi N, Maranhão, Brazil, 3°41'57.3''S 46°46'04.8''W, 131 masl. 22 April 2016, Hernández- García, L.M. & Rousseau G.X. colls . MPEG.ANL 0 0 0 368, one adult, complete. Primary forest , Centro novo do Maranhão, PPBio Gurupi N, Maranhão, Brazil, 3°41'27.7''S 46°46'02.7''W, 146 masl. 23 April 2016, Hernández- García, L.M. & Rousseau G.X. colls . MPEG.ANL 0 0 0 369, one adult, complete. Primary forest , Centro novo do Maranhão, Maranhão, Brazil, 3°41'27.7''S 46°46'02.7''W, 146 masl. 23 April 2016, Hernández-García, L.M. & Rousseau G.X. colls . MPEG.ANL 0 0 0 370, one adult, complete. Pasture, Gurupi settlement, Canãa Village , Itinga do Maranhão, Maranhão, Brazil, 4°2'20.6''S 46°55'58''W, 196 masl. 18 April 2017, Hernández-García, L.M. & Rousseau G.X. colls . MPEG.ANL 0 0 0 371, one adult, complete. Pasture, Gurupi settlement, Canãa Village , Itinga do Maranhão, Maranhão, Brazil, 4°2'18''S 46°54'45.5''W, 158 masl. 20 April 2017, Hernández-García, L.M. & Rousseau G.X. colls . MPEG.ANL 0 0 0 372, three adults, complete. Pasture, Gurupi settlement, Canãa Village , Itinga do Maranhão, Maranhão, Brazil, 4°2'18''S 46°54'45.5''W, 158 masl. 20 April 2017, Hernández-García, L.M. & Rousseau G.X. colls . MPEG.ANL 0 0 0 373, one adult, complete. Degraded forest fragment, Gurupi settlement, Canãa Village, Itinga do Maranhão, Maranhão, Brazil, 4°2'34.5''S 46°54'36.1''W, 164 masl. 20 April 2017, Hernández-García, L.M. & Rousseau G.X. colls . MPEG.ANL 0 0 0 374, one adult, complete. Degraded forest fragment, Gurupi settlement, Canãa Village, Itinga do Maranhão, Maranhão, Brazil, 4°2'34.5''S 46°54'36.1''W, 164 masl. 20 April 2017, Hernández-García, L.M. & Rousseau G.X. colls . MPEG.ANL 0 0 0 375, two adult, complete. Forest fragment, Gurupi settlement, Canãa Village, Itinga do Maranhão, Maranhão, Brazil, 4°5'57.6''S 46°54'48.8''W, 229 masl. 22 April 2017, Hernández-García, L.M. & Rousseau G.X. colls . MPEG.ANL 0 0 0 376, one adult, complete. Forest fragment, Gurupi settlement, Canãa Village, Itinga do Maranhão, Maranhão, Brazil, 4°5'57.6''S 46°54'48.8''W, 229 masl. 22 April 2017, Hernández-García, L.M. & Rousseau G.X. colls . MPEG.ANL 0 0 0 377, one adult, complete. Forest fragment, Gurupi settlement, Canãa Village, Itinga do Maranhão, Maranhão, Brazil, 4°5'57.6''S 46°54'48.8''W, 229 masl. 22 April 2017, Hernández-García, L.M. & Rousseau G.X. colls . MPEG.ANL 0 0 0 378, one adult, complete. Forest fragment, Gurupi settlement, Canãa Village, Itinga do Maranhão, Maranhão, Brazil, 4°6'4''S 46°54'59''W, 185 masl. 22 April 2017, Hernández-García, L.M. & Rousseau G.X. colls . MPEG.ANL 0 0 0 379, one adult, complete. Secondary forest 15 years old, Galetti Farm, Itinga do Maranhão, Maranhão, Brazil, 4°2'9.9''S 46°52'50.2''W, 294 masl. 19 April 2017, Hernández-García, L.M. & Rousseau G.X. colls . MPEG.ANL 0 0 0 380, three juveniles, complete. Pasture, Gurupi settlement, Canãa Village ,, Itinga do Maranhão, Maranhão, Brazil, 4°2'18''S 46°54'45.5''W, 158 masl. 20 April 2017, Hernández-García, L.M. & Rousseau G.X. colls . MPEG.ANL 0 0 0 381, three juveniles, complete. Forest fragment, Gurupi settlement, Canãa Village, Itinga do Maranhão, Maranhão, Brazil, 4°2'34.5''S 46°54'36.1''W, 164 masl. 20 April 2017, Hernández- García, L.M. & Rousseau G.X. colls.

Etymology. The species name is the Guajajara indigenous language word for this earthworm species.

Description. Dimensions: holotype 216 mm (amputee) by 8.3 mm at X, 9.0 mm at clitellum, 6.5 mm at XXX, 203 segments; complete adult paratypes 210–350 mm by 9.0 mm at X, 10.0 mm at clitellum and 7.0 mm at XXX, 221–344 segments. In general the total segments and length are variable because the species presents a biological strategy of self-amputation to survive predator attack. Body cylindrical. Setae ab and cd commence on V and VII, respectively. Setae closely paired throughout; setal formula aa:ab:bc:cd:dd = 6.5:1.0:8.0:0.5:20.0 at XXX, dd> 1/2 circumference throughout. Prostomium tanylobous-tentacular, post-setal secondary annulations present in XXVIII–XXXV and CV–CLXXII behind clitellum. Body pigmented, brownish dorsally and greyish ventrally. Clitellum annular in XV–(1/2) XXVI ( Fig. 2A ) and beige pigmented. Protruding genital markings in XVII, XIX– XXIV with genital setae associated. Genital setae of XXII 3mm in length, ornamented with nine alternate semilunar excavations at the sub-apical concave part ( Fig. 2B ). Two pairs of bands forming the tubercula pubertatis present just near b line between segments XX–XXV. Nephropores intersegmental in cd line. Male and female pores not seen externally, but male sperm ducts enter body wall at 18/19.

Septa equally thick, conical and muscular in 6/7– 8/9 and 11/12. Septa 9/10 and 10/11 absent, while septa 13/ 14–17/18 are thin. Alimentary canal with large cylindrical gizzard in VI; esophagus valvular in XVII, intestinal origin XIII; typhlosole origin XXV and sigmoid form in cross section. Three pairs of calciferous glands with ental appendices in VII–IX, dorsal esophageal connection, garlic clove shaped and lamellar structure ( Fig. 2C,D ). The external part of calciferous gland exposes to coelom is crusty and darker. Holonephric, vesiculate; ducts to body wall near level of cd. Post-clitellar nephridium with one loop connected directly with the bladder compartment ( Fig. 2E ). Vascular system with ventral trunk, single dorsal trunk, lateral vessels in VII–IX. Extra-esophageal vessel from pharyngeal glands, along ventral-lateral face of gizzard, to segment 10. Paired hearts in X–XI, kidneyshaped, surrounded by seminal vesicles and testicular sacs. Supra-esophageal vessels in X–XIII. Ovaries in XIII. Three pairs of spermathecae in segments VII–IX opening in cd line ( Fig. 2F ). Ampulla bean shaped 3.4 x 9 mm in size and connected to the spermathecal pore by a thin tube. Spermathecal ducts as long as the maximum diameter of the ampulla. Male sexual system holandric, testes and funnels in single mid-ventral sub-esophageal sac in each of X and XI; lobulated seminal vesicles occupying segments XI–XIV, XVII. Deferent ducts opening in 18/19.

Remarks. Andiorrhinus (Turedrilus) miricuri n. sp. is similar to Andiorrhinus (Turedrilus) baniwa Righi & Nemeth, 1983 , by the presence of three pairs of spermathecae in segments VII–IX and because both have an annular clitellum. The differences between A. (T.) miricuri n. sp. and A. (Turedrilus) baniwa are, basically, the length of the clitellum; 12 segments (15–1/2 26) in A. (T.) miricuri n. sp. and 11 segments (15–1/2 25) in A. (T.) baniwa (Table 1), the extension of the tubercula pubertatis, which is XX–XXV for A. (T.) miricuri n. sp. and XXI– XXIV for A. (T.) baniwa . On the other hand, the spermathecal duct-ampulla relation is shorter (1:1) in A. (T.) miricuri n. sp. than for A. (T.) baniwa (2:1); the calciferous gland in A. (T.) baniwa is pedunculated while for A. (T.) miricuri ducts are absent; intraclitellar genital papillae are absent or less protruding in A. (T.) baniwa than those of A. (T.) miricuri .

TABLE]. Andiorrhinus (Turedrilus) species comparison.

*Amputee

TABLE] (continued). Andiorrhinus (Turedrilus) species comparison.?: no information available.

Figures

FIGURE 2. Andiorrhinus (Turedrilus) miricuri n. sp. (A–F) and Andiorrhinus (Turedrilus) barrosoi n. sp. (G–K). A. External ventral view of anterior body region, with clitellum, tubercula pubertatis and genital papilla. B. Genital setae of 22. C. View in cross section of right calciferous gland of 9. D. Dorsal view of transversal cut of right calciferous gland of 9, anterior body end to the top. E. View in cross section of left postclitellar nephridium. F. Dorsal view of spermathecae of 8 and 9. G. External ventral view of anterior body region, with clitellum, tubercula pubertatis and genital papilla. H. Genital setae of 20. I. View in cross section of left calciferous gland of 9. J. Dorsal view of transversal cut of right calciferous gland of 9, anterior body end to the top. K. View in cross section of right postclitellar nephridium.